Results 51 to 60 of about 945,082 (262)

Application of the Protoplast Fusion Technique to Engineer a Recombinant Microorganism to More Efficiently Degrade Chlorophenols

open access: yesBioResources, 2015
Recombinant strain Xz6-1 was constructed by the protoplast fusion technique with the goal of endowing it with the ability to efficiently degrade pentachlorophenol (PCP).
Honglei Chen   +4 more
doaj   +1 more source

Protoplast fusion in banana (Musa spp.) : Comparison of chemical (PEG: Polyethylene glycol) and electrical procedure

open access: yes, 2005
Optimization of protoplast fusion parameters is a prerequisite for the establishment of somatic fusion technology for banana breeding. In the present investigations, we compared the most frequently used fusion methods: the electrofusion technique and ...
Foroughi-Wehr, Barbel   +5 more
core   +1 more source

A rapid, highly efficient and economical method of Agrobacterium-mediated in planta transient transformation in living onion epidermis. [PDF]

open access: yesPLoS ONE, 2014
Transient transformation is simpler, more efficient and economical in analyzing protein subcellular localization than stable transformation. Fluorescent fusion proteins were often used in transient transformation to follow the in vivo behavior of ...
Kedong Xu   +13 more
doaj   +1 more source

Optimal Parameters for the Isolation of Carrot Mesophyll Protoplasts In Vitro

open access: yesОвощи России
The isolation of protoplasts from diverse plant species is a widely employed technique. The purpose of this work is to develop an efficient system for isolating and purifying mesophyll protoplasts from Daucus carota.
N. Aljaramany, S. G. Monakhos
doaj   +1 more source

Development and Characterization of Somatic Hybrids of Ulva reticulata Forsskål (×) Monostroma oxyspermum (Kutz.)Doty

open access: yesFrontiers in Plant Science, 2015
Ulvophycean species with diverse trait characteristics provide an opportunity to create novel allelic recombinant variants. The present study reports the development of seaweed variants with improved agronomic traits through protoplast fusion between ...
Vishal eGupta   +2 more
doaj   +1 more source

Induction and Enhancement of Bacteriocin Production (Review)

open access: yesBiotechnology and Bioengineering, EarlyView.
ABSTRACT Conventionally, bacteriocins are produced by microorganisms on complex substrates, released as semi‐purified preparations or crude fermentates. These bacteriocin‐containing products often exhibit unsatisfactory purity, low active‐substance content, and high production costs, limiting their commercialization and use. This review aims to analyze
Oksana Knysh, Artur Martynov
wiley   +1 more source

Construction of acetoin high-producing Bacillus subtilis strain

open access: yesBiotechnology & Biotechnological Equipment, 2016
This paper describes the construction and selection of a high-producing mutant, Bacillus subtilis HB-32, with enhanced acetoin yield and productivity. The mutant was obtained by the protoplast fusion of a Bacillus subtilis mutant TH-49 (Val−) producing ...
Yanjun Tian   +5 more
doaj   +1 more source

Aplikasi Pakan Alami Kaya Karotenoid untuk Post Larvae Penaeus monodon Fab. (Applications Rich Natural Carotenoids Feed for Post Larvae of Penaeus monodon Fab.)

open access: yesIlmu Kelautan, 2013
Udang windu (Penaeus monodon Fab.) merupakan komoditas unggulan Indonesia sedangkan ketersediaan benih udang windu mengalami penurunan mencapai 50% disebabkan serangan berbagai penyakit dan keterbatasan pakan.
Hermin Pancasakti Kusumaningrum   +1 more
doaj   +1 more source

Benchmarking of Fluorescent Proteins in Trichoderma reesei Using Flow Cytometry

open access: yesCytometry Part A, EarlyView.
ABSTRACT Fungi are the subject of extensive research due to their significant impact on human life. However, the development of tools based on fluorescent proteins has been slow in these species. Trichoderma reesei, a filamentous fungus known for its high secretion capability, shows great potential for producing heterologous proteins.
Métivier Killian   +3 more
wiley   +1 more source

Hybridization of Palm Wine Yeasts ( <i>Saccharomyces Cerevisiae</i>) with Brewers Yeast by Protoplast Fusion

open access: yes, 2004
Haploid auxotrophic strains of Saccharomyces cerevisiae were selected from palm wine and propagated by protoplast fusion with Brewers yeast. Fusion resulted in an increase in both ethanol production and tolerance against exogenous ethanol.
OU Ezeronye, Ezeronye, OU
core   +1 more source

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