Results 81 to 90 of about 945,082 (262)
, 2018 Protoplast fusion is a useful technique for citrus genetic improvement. Establishment of protoplast technology could produce triploid seedles citrus and new citrus genetic combination.
Dyah Retno Wulandari +4 more
core +1 more source
Visualising Physcomitrium patens protoplasts with full‐rotation soft X‐ray tomography
Journal of Microscopy, EarlyView.Abstract Soft X‐ray tomography (SXT) is a label‐free, high‐resolution imaging modality that is optimal for 3D visualisation of whole hydrated cells. Although SXT has been extensively used to study remodelling in more than 130 mammalian cell lines, its application to plants remains largely unexplored. In this study, we present the first approach at full‐
Fariha Mahzabin Annesha +4 more
wiley +1 more source
Plant Production Science, 1998 Ultrastructural changes during the fusion of the protoplasts isolated from wild viola callus (without chloroplasts) and pansy mesophyll cells (with chloroplasts) were examined by scanning electron microscopy (SEM) and transmission electron microscopy ...
Tadahiko Sato +7 more
doaj +1 more source
Fusion of yeast protoplasts. [PDF]
Agricultural and Biological Chemistry, 1977 YAMAMOTO, Masahiro, FUKUI, Sakuzo
openaire +2 more sources
Molecular Microbiology, EarlyView.Abbreviated Summary The role of the major fungal cell wall polymer α‐1,3‐glucan in plant infection is poorly understood. Deletion mutants of the maize anthracnose fungus Colletotrichum graminicola lacking all three α‐1,3‐glucan synthase genes differentiated severely distorted biotrophic hyphae in planta, and virulence was strongly reduced.
Iris Gase +13 more
wiley +1 more source
Engineering root development with high precision
New Phytologist, EarlyView.Conceptual framework for precise engineering of root development. Summary Root development is controlled by dynamic interactions between gene regulation, hormonal signalling, developmental history and environmental inputs, creating multiple layers of control that can be harnessed for engineering.
Roza D. Bilas, Sarah Guiziou
wiley +1 more source
Determination of the frequency of interspecific protoplast fusion by differential staining
, 1973 1. The process of spontaneous intraspecific protoplast fusion occurred at high frequencies (20-80%) both during and after cell wall degradation. The expansion of plasmodesmatal strands was considered to be the mechanism involved in achieving spontaneous ...
Gamborg, O. L. +4 more
core
Plant Biotechnology Journal, EarlyView.ABSTRACT Polyploidisation, creating redundant or diverged copies of the genome, is a major driving force in plant evolution, diversification and environmental adaptation, including for the kiwifruit genus (Actinidia Lindl.). We present a high‐contiguity, haplotype‐resolved genome assembly of the hexaploid Actinidia valvata rootstock cultivar ...
Miaomiao Lin +11 more
wiley +1 more source
Validation of a Modular Base Editing Strategy, Free‐BE, in Physcomitrium patens, Maize, and Wheat [PDF]
Plant DirectABSTRACT Base editing (BE) technologies enable precise genome modification but often generate undesired editing outcomes, including indels, bystander edits, and editing outside the optimal activity windows. Here, we report a modular BE strategy, termed Free‐BE, where the cytidine deaminase and Cas9 nickase are delivered as separate components rather ...
Guyon‐Debast A +5 more
europepmc +2 more sources
Single‐Nucleus Transcriptome Analysis Provides New Insights Into B Chromosome Elimination in Sorghum
Plant Biotechnology Journal, EarlyView.ABSTRACT B chromosomes are supernumerary entities found in many plant species, with some exhibiting tissue‐specific elimination. In Sorghum purpureosericeum, extensive B chromosome elimination occurs during embryogenesis affecting most of the embryonic organs, leaving the B chromosome maintained mainly in limited regions of meristems.
T. Bojdová +4 more
wiley +1 more source