Results 141 to 150 of about 38,339 (281)

Protoplast [PDF]

, 2020
openaire   +1 more source

Coupling of both a transactivation module and a double‐stranded DNA‐binding domain boosts Cas12i3 variant‐based cytosine and adenine editing in plants

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3196-3207, September 2026.
Fusions of a transactivation module and a double‐stranded DNA‐binding domain produced effective Cas12i3 variant‐based cytosine and adenine base editors, with improved C‐to‐T and A‐to‐G base editing efficiency and expanded editing window. Generated herbicide‐resistant rice germplasm by using these base editors demonstrates their potential for precise ...
Chen Zhang, Jingying Li, Yucai Li, Lei Yan, Christina Seok Yien Yong, Shaoya Li, Yubing He, Lanqin Xia   +7 more
wiley   +1 more source

Fusion of yeast protoplasts. [PDF]

Agricultural and Biological Chemistry, 1977
YAMAMOTO, Masahiro, FUKUI, Sakuzo
openaire   +2 more sources

Transient expression of foreign genes introduced into barley endosperm protoplasts by PEG-mediated transfer or into intact endosperm tissue by microprojectile bombardment

, 1991
Starchy endosperm protoplasts from developing barley (Hordeum vulgare) grains were isolated 8–15 days post-anthesis. These endosperm protoplasts were incubated in a hormone free medium containing sucrose as a carbon source and glutamine as a nitrogen ...
Kreis, M., Topping, J., Lee, B.T., Lee, B. T., Jones, M.G.K., Murdoch, K., Jones, M. G. K.   +6 more
core  

Enhanced exonuclease–Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3316-3328, September 2026.
Cas9 preferentially generates small indels. By tethering an exonuclease and a DNA‐binding domain (DBD) to Cas9, the Exo‐DBD‐Cas9 system efficiently promotes multiple nucleotide deletions. These expanded deletions enable effective disruption of miRNA‐mediated regulation and relieve translational repression through targeted editing of 3′ untranslated ...
Rui Zhang, Xu Tang, Yao He, Wei Wang, Qiurong Ren, Yiping Qi, Yong Zhang   +6 more
wiley   +1 more source

Synergistic engineering of Casδ nuclease for robust genome editing

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3231-3242, September 2026.
A hierarchical engineering strategy was used to enhance the editing activity of Casδ, yielding an optimized variant termed enCasδ. This variant enables robust genome editing in animal cells and plants, with overall editing performance comparable to that of SpCas9 and other Cas12 nucleases.
Fanghui Ge, Chenchen Peng, Yang Du, Ying Chen, Zilong Zhao, Meixia Yu, Huairu Feng, Yuyang Xie, Siwei Sun, Shengnan Liu, Beibei Xin, Haiming Zhao, Sen Wu, Chao Bian, Zhijia Yang, Jinsheng Lai, Jian Chen   +16 more
wiley   +1 more source

Genetic transformation and CRISPR/Cas12a-mediated gene editing of European beech (Fagus sylvatica L.) employing a transient protoplast system. [PDF]

Commun Biol
Zahn V, Sievers AJ, Kersten B, Fladung M, Bruegmann T.   +4 more
europepmc   +1 more source

SIB1‐SEC23A undergo ER to chloroplast relocalization to mediate immunity in Arabidopsis thaliana

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3458-3472, September 2026.
SEC23A mediates membrane trafficking between the endoplasmic reticulum (ER) and Golgi apparatus and interacts with the immunity protein SIGMA FACTOR‐BINDING PROTEIN1 in Arabidopsis. Under stress, both proteins relocate from the ER to chloroplasts, suppressing photosynthesis genes and enhancing defense genes.
Jialin Peng, Huan Zhong, Jianan Zhang, Hailei Zhang, Wuzhen Liu, Yonglun Zeng, Liwen Jiang, Yiji Xia   +7 more
wiley   +1 more source

Optimized Cas‐SF01 gene‐editing toolbox shortens flowering timing in commercial maize inbred JING724

Journal of Integrative Plant Biology, Volume 68, Issue 9, Page 3178-3180, September 2026.
The gene‐editing tool Cas‐SF01 was optimized to maximize its efficiency in maize. The Cas‐SF01‐TREX2 configuration was superior in enabling high‐purity gene mutations. This toolkit enabled commercial maize to flower seven days earlier without yield loss, thereby securing harvests and accelerating crop breeding.
Mengyuan Liu, Yao Wang, Lu Zhang, Xiang Zhang, Xiaqing Wang, Xinxiang Liu, Yue Fu, Xiaoxu Li, Ziheng Song, Ya Liu, Ronghuan Wang, Jiuran Zhao   +11 more
wiley   +1 more source

Research Progress on Preparation, Transformation and Application of Protoplasts Derived from Medicinal Plants. [PDF]

Plants (Basel)
Fang Z, Hu Y, Zhou Z, Ma H, Zhang L, Peng Y, Zhan X, Sun Y, Shen C.   +8 more
europepmc   +1 more source