Results 171 to 180 of about 14,046 (214)

Crystallization of exotoxin A from Pseudomonas aeruginosa

Journal of Molecular Biology, 1982
Abstract Exotoxin A from Pseudomonas aeruginosa has been crystallized in a form suitable for high resolution diffraction analysis. The crystals, grown in the presence of high concentrations of polyethylene glycol (20%, w/v) and of NaCl (1.5 m ), are monoclinic and contain one monomeric toxin molecule per asymmetric unit. The space group is P 2 1 ,
R J, Collier, D B, McKay
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Pseudomonas Aeruginosa Exotoxin a Induces Apoptosis in Galleria Mellonella Hemocytes

SSRN Electronic Journal, 2022
The cellular immune response of the greater wax moth Galleria mellonella to Pseudomonas aeruginosa exotoxin A was investigated for the first time. The insects were challenged with a sublethal dose of exoA, and then hemocyte parameters were assessed. The analysis showed a statistically significant decrease in the total hemocyte count (THC), which was ...
Bartłomiej, Iwański, Magdalena, Mizerska-Kowalska, Mariola, Andrejko   +2 more
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Exotoxins of Pseudomonas aeruginosa. II. Concentration, Purification, and Characterization of Exotoxin A

Journal of Infectious Diseases, 1973
Exotoxin A of Pseudomonas aeruginosa (PA-103) could be concentrated readily by precipitation with zinc acetate and ammonium sulfate. The toxin was purified by column chromatography with use of DEAE-cellulose and Sephadex G-200. The final product contained about 8,000 mouse LD,,0/mg of protein.
P V, Liu, S, Yoshii, H, Hsieh
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Pseudomonas aeruginosaExotoxin A

New England Journal of Medicine, 1980
Pseudomonas aeruginosa is an opportunistic pathogen that causes more than 100,000 infections in the United States each year. Pseudomonas infections are associated with considerable morbidity and mortality despite the use of modern antibiotics.1 Recognition of the limitations of existing therapy for pseudomonas disease has stimulated renewed interest in
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The acid-triggered entry pathway of Pseudomonas exotoxin A

Biochemistry, 1989
In this study we examined the pH requirements and reversibility of early events in the Pseudomonas toxin entry pathway, namely, membrane binding, insertion, and translocation. At pH 7.4, toxin binding to vesicles and insertion into the bilayer are very inefficient. Decreasing the pH greatly increases the efficiencies of these processes.
Z T, Farahbakhsh, B J, Wisnieski
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Resistance of Exotoxin A to Purified Pseudomonas Proteolytic Enzymes

Infection and Immunity, 1980
Pseudomonas toxin is produced as a proenzyme which is cytotoxic for cells in culture but must be activated to express full enzymatic activity. The ability of purified pseudomonas alkaline protease and elastase or of culture filtrates of two strains of Pseudomonas aeruginosa to modify the activity of pseudomonas ...
K, Jagger, M M, Nickol, C B, Saelinger
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Immunotoxins containing Pseudomonas exotoxin A: a short history

Cancer Immunology, Immunotherapy, 2003
A large variety of monoclonal antibodies (MAb) have been identified that bind to antigens on cancer cells and there are many mechanisms by which antibodies can kill cancer cells. However, very few antibodies are able to kill sufficient numbers of cells to cause tumor regression in experimental animals and even fewer are useful in the treatment of ...
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[21] Preparation of Pseudomonas exotoxin A

1988
Publisher Summary This chapter describes the preparation of Pseudornonas exotoxin A (PE). PE is one of the major virulence factors of Pseudomonas aeruginosa . A low protease-producing strain PAl03 is usually used for exotoxin production. The undefined growth medium for PE production is the dialysate of trypticase soy broth (TSB).
Kenneth J Kozak, Catharine B Saelinger
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Regulation of Pseudomonas Aeruginosa Exotoxin a Synthesis

2004
Exotoxin A (ETA) is one of the most toxic virulence factors produced by Pseudomonas aeruginosa 72: Both clinical and experimental animal studies indicate clearly the importance of ETA in the pathogenesis of different P. aeruginosa infections. Most P. aeruginosa clinical isolates produce ETA9, 23, 47.
Abdul N. Hamood, Jane A. Colmer-Hamood, Nancy L. Carty   +2 more
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Pseudomonas aeruginosa exotoxin A

1997
Abstract Pseudomonas aeruginosa exotoxin A (PEA, MW 66 kDa, 613 amino acids, sequence accession number: PSEETA K01397) consists of three major domains (Allured et al. 1986), as indicated in Fig. 1. The N-terminal domain la (amino acids 1-252) binds to the ai-macroglobulin receptor at the cell surface (Kounnas et al.
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