Results 51 to 60 of about 431,100 (179)

The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules. [PDF]

J Biol Chem, 2017
Mitochondrial gene expression is a fundamental process that is largely dependent on nuclear-encoded proteins. Several steps of mitochondrial RNA processing and maturation, including RNA post-transcriptional modification, appear to be spatially organized into distinct foci, which we have previously termed mitochondrial RNA granules (MRGs).
Zaganelli S, Rebelo-Guiomar P, Maundrell K, Rozanska A, Pierredon S, Powell CA, Jourdain AA, Jourdain AA, Hulo N, Lightowlers RN, Chrzanowska-Lightowlers ZM, Minczuk M, Martinou JC.   +12 more
europepmc   +8 more sources

Pseudouridine mapping in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (snRNAs) reveals that pseudouridine synthase pus1p exhibits a dual substrate specificity for U2 snRNA and tRNA.

, 1999
Pseudouridine (Psi) residues were localized in the Saccharomyces cerevisiae spliceosomal U small nuclear RNAs (UsnRNAs) by using the chemical mapping method. In contrast to vertebrate UsnRNAs, S.
Massenet, S, Branlant, C, Grosjean, Henri, Hurt, Eduard, Lafontaine, Denis, Massenet, Séverine, Branlant, Christiane, Hurt, Eduard C., Motorin, Y, Motorin, Yuri   +9 more
core   +1 more source

The Handling of the Mechanistic Probe 5-Fluorouridine by the Pseudouridine Synthase TruA and Its Consistency with the Handling of the Same Probe by the Pseudouridine Synthases TruB and RluA [PDF]

Biochemistry, 2010
RNA containing 5-fluorouridine (F(5)U) had previously been used to examine the mechanism of the pseudouridine synthase TruA, formerly known as pseudouridine synthase I [Gu et al. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 14270-14275]. From that work, it was reasonably concluded that the pseudouridine synthases proceed via a mechanism involving a Michael
Marguerite K, McDonald, Edward J, Miracco, Junjun, Chen, Yizhou, Xie, Eugene G, Mueller   +4 more
openaire   +2 more sources

Interrogating the Escherichia coli Epitranscriptome Via CRISPR Interference and Nanopore Native RNA Sequencing

MicrobiologyOpen, Volume 15, Issue 5, October 2026.
Epitranscriptomics is understudied in bacteria but has the ability to modulate antimicrobial resistance, virulence and pathogenicity. Pitt et al. implement two cutting‐edge techniques: CRISPR interference and Nanopore direct RNA sequencing along with functional assays to study RNA modifications in Escherichia coli.
Miranda E. Pitt, Jianshu Zhang, An N. T. Nguyen, Michael B. Hall, Leila Jebeli, Leo A. Featherstone, Garry S. A. Myers, Nichollas E. Scott, Lachlan J. M. Coin   +8 more
wiley   +1 more source

Pseudouridine synthase 1: a site-specific synthase without strict sequence recognition requirements. [PDF]

Nucleic Acids Res, 2012
Pseudouridine synthase 1 (Pus1p) is an unusual site-specific modification enzyme in that it can modify a number of positions in tRNAs and can recognize several other types of RNA. No consensus recognition sequence or structure has been identified for Pus1p.
Sibert BS, Patton JR.
europepmc   +4 more sources

Multiple pseudouridine synthase activities for small nuclear RNAs [PDF]

Biochemical Journal, 1993
The formation of pseudouridine (psi) in human U1, U2 and U5 small nuclear RNAs (snRNAs) was investigated using HeLa cell extracts. Unmodified snRNAs were synthesized in vitro and the extent of psi formation was determined after incubation in cell extracts. The formation of psi on labelled substrates was monitored in the presence of 5-fluorouracil (5-FU)
openaire   +2 more sources

Crystal structure of TruD, a novel pseudouridine synthase with a new protein fold

, 2004
TruD, a recently discovered novel pseudouridine synthase in Escherichia coli, is responsible for modifying uridine13 in tRNA(Glu) to pseudouridine. It has little sequence homology with the other 10 pseudouridine synthases in E. coli which themselves have
Kaya, Yusuf, Malhotra, Arun, Ofengand, James, Del Campo, Mark   +3 more
core   +1 more source

How Epitranscriptomic Machinery Senses Environmental Cues

Advanced Biology, Volume 10, Issue 9, September 2026.
Exogenous exposures, microenvironmental signals, and intracellular metabolic or redox states can alter RNA‐modification machinery through catalytic chemistry, signaling‐dependent PTMs, compartmental redistribution, or changes in abundance and composition.
Shayan Jalali, Ehsan Pashay Ahi
wiley   +1 more source

Cerium Nanoparticle‐Mediated Inhibition of the NSUN2/m5C Axis Suppresses Synovial Aggression in Rheumatoid Arthritis

Advanced Science, Volume 13, Issue 54, 28 September 2026.
A novel epitranscriptomic mechanism in rheumatoid arthritis is uncovered: NSUN2 promotes disease via m5C‐dependent stabilization of ICMT mRNA, fueling the migration and invasion of pathogenic RA FLS. Targeting this axis with engineered nanoparticles (Ce/SAA NPs) effectively inhibits disease progression, presenting a precise therapeutic strategy ...
Ruiru Li, Simin Chen, Huijuan Hu, Shiyao Wu, Jingnan Wang, Kai Sun, Chenxi Peng, Suling Liu, Shuoyang Zhang, Yu Kuang, Wei Chen, Qian Qiu, Liuqin Liang, Yu Wen, Hanshi Xu, Youjun Xiao   +15 more
wiley   +1 more source

Mechanistic insight into the pseudouridylation of RNA

RNA Biology
Pseudouridylation (Ψ) is a highly abundant and conserved RNA modification that is present in all three domains of life. The incorporation of Ψ can affect the stability of RNAs, modulate their interaction patterns, and regulate many other aspects of RNA ...
Ting-Yu Lin, Yasmin Stone, Sebastian Glatt   +2 more
doaj   +1 more source