Chemo-Enzymatic Generation of Highly Fluorescent Nucleoside Analogs Using Purine-Nucleoside Phosphorylase [PDF]
BiomoleculesChemo-enzymatic syntheses of strongly fluorescent nucleoside analogs, potentially applicable in analytical biochemistry and cell biology are reviewed. The syntheses and properties of fluorescent ribofuranosides of several purine, 8-azapurine, and etheno ...
Alicja Stachelska-Wierzchowska +1 more
doaj +2 more sources
[Purine nucleoside phosphorylase]. [PDF]
Biomeditsinskaya Khimiya, 2013 Purine nucleoside phosphorylase (PNP) is one of the most important enzymes of the purine metabolism, wich promotes the recycling of purine bases. Nowadays is the actual to search for effective inhibitors of this enzyme which is necessary for creation T ...
L. Pogosian, Zh I Akopian
semanticscholar +3 more sources
Structural determinants of the 5'-methylthioinosine specificity of Plasmodium purine nucleoside phosphorylase. [PDF]
PLoS ONE, 2014 Plasmodium parasites rely upon purine salvage for survival. Plasmodium purine nucleoside phosphorylase is part of the streamlined Plasmodium purine salvage pathway that leads to the phosphorylysis of both purines and 5'-methylthiopurines, byproducts of ...
Teraya M Donaldson +6 more
doaj +2 more sources
Genetic resistance to purine nucleoside phosphorylase inhibition in Plasmodium falciparum. [PDF]
Proc Natl Acad Sci U S A, 2018 Significance Hypoxanthine salvage is essential for nucleic acid synthesis in Plasmodium falciparum but not in humans. Hypoxanthine production in P. falciparum can be blocked by DADMe-Immucillin-G (DADMe-ImmG), a transition-state analog inhibitor of ...
Ducati RG +6 more
europepmc +2 more sources
Plasmodium falciparum parasites are killed by a transition state analogue of purine nucleoside phosphorylase in a primate animal model. [PDF]
PLoS ONE, 2011 Plasmodium falciparum causes most of the one million annual deaths from malaria. Drug resistance is widespread and novel agents against new targets are needed to support combination-therapy approaches promoted by the World Health Organization. Plasmodium
María B Cassera +11 more
doaj +5 more sources
Evaluating antitumor activity of Escherichia coli purine nucleoside phosphorylase against head and neck patient‐derived xenografts [PDF]
Cancer Reports, 2023 Background Purine nucleoside phosphorylase (PNP) gene transfer represents a promising approach to treatment of head and neck malignancies. We tested recombinant adenovirus already in phase I/II clinical testing and leading‐edge patient‐derived xenografts
Regina Rab +10 more
doaj +2 more sources
Purine nucleoside phosphorylase controls nicotinamide riboside metabolism in mammalian cells. [PDF]
J Biol Chem, 2022 Kropotov A +13 more
europepmc +2 more sources
Synthesis of Substituted 1,2,4-Triazole-3-Thione Nucleosides Using E. coli Purine Nucleoside Phosphorylase [PDF]
Biomolecules1,2,4-Triazole derivatives have a wide range of biological activities. The most well-known drug that contains 1,2,4-triazole as part of its structure is the nucleoside analogue ribavirin, an antiviral drug. Finding new nucleosides based on 1,2,4-triazole
Ilya V. Fateev +22 more
doaj +2 more sources
Inverse enzyme isotope effects in human purine nucleoside phosphorylase with heavy asparagine labels. [PDF]
Proc Natl Acad Sci U S A, 2018 Significance Enzymes achieve catalytic efficiency by optimizing contacts between reactants and catalytic site amino acids. The transition state forms rarely, with a lifetime of a few femtoseconds.
Harijan RK +4 more
europepmc +2 more sources
Partial Purine Nucleoside Phosphorylase Deficiency Helps Determine Minimal Activity Required for Immune and Neurological Development [PDF]
Frontiers in Immunology, 2020 Introduction: Complete or near complete absence of the purine nucleoside phosphorylase (PNP) enzyme causes a profound T cell immunodeficiency and neurological abnormalities that are often lethal in infancy and early childhood.
Eyal Grunebaum +5 more
doaj +2 more sources