Results 61 to 70 of about 24,446 (248)

A β-Fluoroamine Inhibitor of Purine Nucleoside Phosphorylase [PDF]

Journal of Medicinal Chemistry, 2008
The potent immucillin purine nucleoside phosphorylase (PNP ) inhibitors F-DADMe-ImmH [(3S,4S)-3], and [(3R,4R)-3] are synthesized in seven steps. Cycloaddition to a fluoroalkene and an enzymic resolution are the key features of the construction of the fluoropyrrolidines 11, from which the immucillins are assembled by use of a three-component Mannich ...
Graeme J. Gainsford, Andrew S. Murkin, Brian W. Skelton, Lei Li, Jennifer M. Mason, Vern L. Schramm   +5 more
openaire   +3 more sources

Intracellular Delivery of Human Purine Nucleoside Phosphorylase by Engineered Diphtheria Toxin Rescues Function in Target Cells.

Molecular Pharmaceutics, 2018
Despite a wealth of potential applications inside target cells, protein-based therapeutics are largely limited to extracellular targets due to the inability of proteins to readily cross biological membranes and enter the cytosol.
Minyoung Park, Xiaobai Xu, W. Min, S. Sugiman-Marangos, Greg L. Beilhartz, Jarrett J. Adams, S. Sidhu, E. Grunebaum, R. Melnyk   +8 more
semanticscholar   +1 more source

Investigating the Role of Guanosine on Human Neuroblastoma Cell Differentiation and the Underlying Molecular Mechanisms

Frontiers in Pharmacology, 2021
Neuroblastoma arises from neural crest cell precursors failing to complete the process of differentiation. Thus, agents helping tumor cells to differentiate into normal cells can represent a valid therapeutic strategy.
Natale Belluardo, Giuseppa Mudò, Valentina Di Liberto, Monica Frinchi, Daniele F. Condorelli, Ugo Traversa, Francisco Ciruela, Francisco Ciruela, Renata Ciccarelli, Renata Ciccarelli, Patrizia Di Iorio, Patrizia Di Iorio, Patricia Giuliani, Patricia Giuliani   +13 more
doaj   +1 more source

Enzymatic Synthesis of M1Gâ Deoxyribose [PDF]

, 2000
Adducts formed between electrophiles and nucleic acid bases are believed to play a key role in chemically induced mutations and cancer. M1Gâ dR is an endogenous exocyclic DNA adduct formed by the reaction of the dicarbonyl compound malondialdehyde with ...
Chapeau, Marie‐christine, Marnett, Lawrence J., Schnetz‐boutaud, Nathalie C.   +2 more
core   +1 more source

The Regulation of Brain Nucleoside Utilization [PDF]

, 2014
The homeostatic regulation of intracellular purine and pyrimidine pools has long been studied at the level of de novo nucleotide synthesis. However, brain maintains the proper qualitative and quantitative nucleotide balance by salvaging preformed ...
BALESTRI, FRANCESCO, IPATA, PIERO LUIGI
core   +1 more source

A specific adenosine phosphorylase, distinct from purine nucleoside phosphorylase

FEBS Letters, 1976
Several enzymes, catalyzing the reversible phosphorolysis of purine nucleosides have been described in eucaryotic cells and in micro-organisms [l-6] . The best known is the ‘purine nucleoside phosphorylase’ (EC 2.4.2.1) acting on the nucleosides of hypoxanthine and guanine. Adenosine is not used as substrate by this enzyme [6-81.
SENESI, SONIA, FALCONE, GIUSEPPE, MURA, UMBERTO, SGARRELLA F, IPATA, PIERO LUIGI   +4 more
openaire   +6 more sources

Plasmodium Purine Metabolism and Its Inhibition by Nucleoside and Nucleotide Analogues [PDF]

, 2019
International audienceMalaria still affects around 200 million people and is responsible for more than 400,000 deaths per year, mostly children in subequatorial areas. This disease is caused by parasites of the Plasmodium genus.
Cheviet, Thomas, Lefebvre-Tournier, Isabelle, Peyrottes, Suzanne, Wein, Sharon   +3 more
core   +2 more sources

Sustainable Flow‐Synthesis of (Bulky) Nucleoside Drugs by a Novel and Highly Stable Nucleoside Phosphorylase Immobilized on Reusable Supports

ChemSusChem, 2021
The continuous synthesis of valuable nucleoside drugs was achieved in up to 99 % conversion by using a novel halotolerant purine nucleoside phosphorylase from Halomonas elongata (HePNP).
Ana I. Benítez-Mateos, F. Paradisi
semanticscholar   +1 more source

Engineering a Bifunctional Fusion Purine/Pyrimidine Nucleoside Phosphorylase for the Production of Nucleoside Analogs

Biomolecules
Nucleoside phosphorylases (NPs) are pivotal enzymes in the salvage pathway, catalyzing the reversible phosphorolysis of nucleosides to produce nucleobases and α-D-ribose 1-phosphate.
Daniel Hormigo, Jon Del Arco, Javier Acosta, Maximilian J. L. J. Fürst, Jesús Fernández-Lucas   +4 more
doaj   +1 more source

Insights into phosphate cooperativity and influence of substrate modifications on binding and catalysis of hexameric purine nucleoside phosphorylases. [PDF]

PLoS ONE, 2012
The hexameric purine nucleoside phosphorylase from Bacillus subtilis (BsPNP233) displays great potential to produce nucleoside analogues in industry and can be exploited in the development of new anti-tumor gene therapies.
Priscila O de Giuseppe, Nadia H Martins, Andreia N Meza, Camila R dos Santos, Humberto D'Muniz Pereira, Mario T Murakami   +5 more
doaj   +1 more source