Results 1 to 10 of about 12,609 (258)

Early Diagnosis and Treatment of Purine Nucleoside Phosphorylase (PNP) Deficiency through TREC-Based Newborn Screening [PDF]

International Journal of Neonatal Screening, 2021
Purine nucleoside phosphorylase (PNP) deficiency is a rare inherited disorder, resulting in severe combined immunodeficiency. To date, PNP deficiency has been detected in newborn screening only through the use of liquid chromatography tandem mass ...
Andrea Martín-Nalda, Jacques G. Rivière, Mireia Català-Besa, Marina García-Prat, Alba Parra-Martínez, Mónica Martínez-Gallo, Roger Colobran, Ana Argudo-Ramírez, Jose Luis Marín-Soria, Judit García-Villoria, Laura Alonso, Jose Antonio Arranz-Amo, Giancarlo la Marca, Pere Soler-Palacín   +13 more
doaj   +8 more sources

The structure of His-tagged Geobacillus stearothermophilus purine nucleoside phosphorylase reveals a `spanner in the works'. [PDF]

Acta Crystallogr F Struct Biol Commun, 2022
The structure of Geobacillus stearothermophilus purine nucleoside phosphorylase, an enzyme of biocatalytic interest, is reported and was found to include the presence of an N‐terminal tag in the active site of each subunit that belongs to the other subunit in each dimer.
Given FM, Moran F, Johns AS, Titterington JA, Allison TM, Crittenden DL, Johnston JM.   +6 more
europepmc   +2 more sources

Synthesis of New 5′-Norcarbocyclic Aza/Deaza Purine Fleximers - Noncompetitive Inhibitors of E.coli Purine Nucleoside Phosphorylase [PDF]

Frontiers in Chemistry, 2022
A new series of flexible 5′-norcarbocyclic aza/deaza-purine nucleoside analogs were synthesized from 6-oxybicyclo[3.1.0.]hex-2-ene and pyrazole-containing fleximer analogs of heterocyclic bases using the Trost procedure.
Anastasia Khandazhinskaya, Ilja Fateev, Irina Konstantinova, Roman Esipov, Konstantin Polyakov, Katherine Seley-Radtke, Sergey Kochetkov, Elena Matyugina   +7 more
doaj   +2 more sources

An Enzymatic Flow-Based Preparative Route to Vidarabine [PDF]

Molecules, 2020
The bi-enzymatic synthesis of the antiviral drug vidarabine (arabinosyladenine, ara-A), catalyzed by uridine phosphorylase from Clostridium perfringens (CpUP) and a purine nucleoside phosphorylase from Aeromonas hydrophila (AhPNP), was re-designed under ...
Lucia Tamborini, Clelia Previtali, Francesca Annunziata, Teodora Bavaro, Marco Terreni, Enrica Calleri, Francesca Rinaldi, Andrea Pinto, Giovanna Speranza, Daniela Ubiali, Paola Conti   +10 more
doaj   +6 more sources

PNP (Purine Nucleoside Phosphorylase) [PDF]

Atlas of Genetics and Cytogenetics in Oncology and Haematology, 2018
The purine nucleoside phosphorylase gene (PNP) encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. PNP is ubiquitously expressed in mammalian cells and tissues.
Gurbanov, Rafig, Tunçer, Sinem
core   +4 more sources

The purine nucleoside phosphorylase pnp-1 regulates epithelial cell resistance to infection in C. elegans. [PDF]

PLoS Pathogens, 2021
Intestinal epithelial cells are subject to attack by a diverse array of microbes, including intracellular as well as extracellular pathogens. While defense in epithelial cells can be triggered by pattern recognition receptor-mediated detection of microbe-
Eillen Tecle, Crystal B Chhan, Latisha Franklin, Ryan S Underwood, Wendy Hanna-Rose, Emily R Troemel   +5 more
doaj   +2 more sources

Chemo-Enzymatic Generation of Highly Fluorescent Nucleoside Analogs Using Purine-Nucleoside Phosphorylase [PDF]

Biomolecules
Chemo-enzymatic syntheses of strongly fluorescent nucleoside analogs, potentially applicable in analytical biochemistry and cell biology are reviewed. The syntheses and properties of fluorescent ribofuranosides of several purine, 8-azapurine, and etheno ...
Alicja Stachelska-Wierzchowska, Jacek Wierzchowski   +1 more
doaj   +2 more sources

Purine nucleoside phosphorylase enables dual metabolic checkpoints that prevent T cell immunodeficiency and TLR7-associated autoimmunity [PDF]

The Journal of Clinical Investigation, 2022
Purine nucleoside phosphorylase (PNP) enables the breakdown and recycling of guanine nucleosides. PNP insufficiency in humans is paradoxically associated with both immunodeficiency and autoimmunity, but the mechanistic basis for these outcomes is ...
Evan R. Abt, Khalid Rashid, Thuc M. Le, Suwen Li, Hailey R. Lee, Vincent Lok, Luyi Li, Amanda L. Creech, Amanda N. Labora, Hanna K. Mandl, Alex K. Lam, Arthur Cho, Valerie Rezek, Nanping Wu, Gabriel Abril-Rodriguez, Ethan W. Rosser, Steven D. Mittelman, Willy Hugo, Thomas Mehrling, Shanta Bantia, Antoni Ribas, Timothy R. Donahue, Gay M. Crooks, Ting-Ting Wu, Caius G. Radu   +24 more
doaj   +2 more sources

Efficient Synthesis of Purine Nucleoside Analogs by a New Trimeric Purine Nucleoside Phosphorylase from Aneurinibacillus migulanus AM007 [PDF]

Molecules, 2019
Purine nucleoside phosphorylases (PNPs) are promising biocatalysts for the synthesis of purine nucleoside analogs. Although a number of PNPs have been reported, the development of highly efficient enzymes for industrial applications is still in high ...
Gaofei Liu, Tiantong Cheng, Jianlin Chu, Sui Li, Bingfang He   +4 more
doaj   +2 more sources

Plasmodium falciparum parasites are killed by a transition state analogue of purine nucleoside phosphorylase in a primate animal model. [PDF]

PLoS ONE, 2011
Plasmodium falciparum causes most of the one million annual deaths from malaria. Drug resistance is widespread and novel agents against new targets are needed to support combination-therapy approaches promoted by the World Health Organization. Plasmodium
María B Cassera, Keith Z Hazleton, Emilio F Merino, Nicanor Obaldia, Meng-Chiao Ho, Andrew S Murkin, Richard DePinto, Jemy A Gutierrez, Steven C Almo, Gary B Evans, Yarlagadda S Babu, Vern L Schramm   +11 more
doaj   +5 more sources