Pyrimidine biosynthesis is not an essential function for trypanosoma brucei bloodstream forms [PDF]
, 2013 <p>Background: African trypanosomes are capable of both pyrimidine biosynthesis and salvage of preformed pyrimidines from the host, but it is unknown whether either process is essential to the parasite.</p> <p>Methodology/Principal ...
A Hofer +51 more
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Crystallographic snapshots of ligand binding to hexameric purine nucleoside phosphorylase and kinetic studies give insight into the mechanism of catalysis. [PDF]
Sci Rep, 2018 Purine nucleoside phosphorylase (PNP) catalyses the cleavage of the glycosidic bond of purine nucleosides using phosphate instead of water as a second substrate.
Štefanić Z +5 more
europepmc +2 more sources
Use of E. coli Purine Nucleoside Phosphorylase in the Treatment of Solid Tumors. [PDF]
Curr Pharm Des, 2017 BACKGROUND The selective expression of non-human genes in tumor tissue to activate non-toxic compounds (Gene Directed Prodrug Enzyme Therapy, GDEPT) is a novel strategy designed for killing tumor cells in patients with little or no systemic toxicity ...
Parker WB, Sorscher EJ.
europepmc +2 more sources
Propolis Exerts an Anti-Inflammatory Effect on PMA-Differentiated THP-1 Cells via Inhibition of Purine Nucleoside Phosphorylase. [PDF]
Metabolites, 2019 Previous research has shown that propolis has immunomodulatory activity. Propolis extracts from different geographic origins were assessed for their anti-inflammatory activities by investigating their ability to alter the production of tumour necrosis ...
Alqarni AM +6 more
europepmc +2 more sources
Structure of purine nucleoside phosphorylase (DeoD) from Bacillus anthracis [PDF]
, 2005 Protein structures from the causative agent of anthrax (Bacillus anthracis) are being determined as part of a structural genomics programme. Amongst initial candidates for crystallographic analysis are enzymes involved in nucleotide biosynthesis, since ...
Blagova, E V +6 more
core +3 more sources
Purine nucleoside phosphorylase: A new marker for free oxygen radical injury to the endothelial cell [PDF]
, 1990 The effect of ischemia and reperfusion on purine nucleoside phosphorylase was studied in an isolated perfused rat liver model. This enzyme is localized primarily in the cytoplasm of the endothelial and Kupffer cells; some activity is associated with the ...
Abouna +32 more
core +1 more source
Targeting purine metabolism in ovarian cancer
Journal of Ovarian Research, 2022 Purine, an abundant substrate in organisms, is a critical raw material for cell proliferation and an important factor for immune regulation. The purine de novo pathway and salvage pathway are tightly regulated by multiple enzymes, and dysfunction in ...
Jingchun Liu +7 more
doaj +1 more source
An unusual presentation of purine nucleoside phosphorylase deficiency mimicking systemic juvenile idiopathic arthritis complicated by macrophage activation syndrome. [PDF]
Pediatr Rheumatol Online J, 2019 Arduini A +9 more
europepmc +3 more sources
Functional and genetic evidence that nucleoside transport is highly conserved in Leishmania species: Implications for pyrimidine-based chemotherapy [PDF]
, 2017 Leishmania pyrimidine salvage is replete with opportunities for therapeutic intervention with enzyme inhibitors or antimetabolites. Their uptake into cells depends upon specific transporters; therefore it is essential to establish whether various ...
Ali, Juma A.M. +7 more
core +2 more sources
Science Translational Medicine, 2019 A cellular thermal shift assay (CETSA) protocol identifies and resolves antimalarial drug targets in P. falciparum. Defining drug targets in malaria Different classes of malaria drugs have been used for decades, even though the mechanisms of action have ...
J. Dziekan +9 more
semanticscholar +1 more source