Real-Time Monitoring of NIH/3T3 Cell Growth and Drug Reaction Using Impedance Biosensors and Comparison with Biological Assays [PDF]
BiosensorsImpedance biosensors are manufactured on glass slides using a semiconductor process to monitor cell growth and cell–drug reactions in real time, and the results are compared with biological assay results to confirm the validity of impedance measurement ...
Seok-kyu Kim +4 more
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Quantitative IC50 Analysis of Puromycin-Induced Cytotoxicity in NIH/3T3 Cells Using a Multi-Well Array Impedance Biosensor [PDF]
BiosensorsECIS-based impedance biosensors have been extensively studied in various fields including cancer research, microbiology, and immunology. However, most studies have primarily focused on monitoring cellular behavior through impedance changes, with ...
Seok-kyu Kim, SuGwon Nam, Moongyu Jang
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Puromycin aminonucleoside modulates p130Cas of podocytes [PDF]
Korean Journal of Pediatrics, 2012 PurposePuromycin aminonucleoside (PAN) specifically injures podocytes, leading to foot process effacement, actin cytoskeleton disorganization, and abnormal distribution of slit diaphragm proteins. p130Cas is a docking protein connecting F-actin fibers to
Tae-Sun Ha +2 more
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Sertraline treatment prevents motor dysfunction in a Huntington's disease mouse model and functional decline in patients [PDF]
NeurotherapeuticsMolecular alterations underlying Huntington's disease (HD) are not fully elucidated and no curative therapies are available. We have described that increased translation efficiency participates in the motor symptoms in the R6/1 HD mouse model.
Marta Garcia-Forn +11 more
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Rapid cell type-specific nascent proteome labeling in Drosophila
eLife, 2023 Controlled protein synthesis is required to regulate gene expression and is often carried out in a cell type-specific manner. Protein synthesis is commonly measured by labeling the nascent proteome with amino acid analogs or isotope-containing amino ...
Stefanny Villalobos-Cantor +6 more
doaj +1 more source
Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish
Animal Cells and Systems, 2023 Puromycin treatment can cause glomerular injury to the kidney, leading to proteinuria. However, the pathogenesis of acute kidney injury and subsequent regeneration after puromycin administration in animal models remain unclear.
Soonil Koun +5 more
doaj +1 more source
eLife, 2020 Puromycin is an amino-acyl transfer RNA analog widely employed in studies of protein synthesis. Since puromycin is covalently incorporated into nascent polypeptide chains, anti-puromycin immunofluorescence enables visualization of nascent protein ...
Benjamin D Hobson +4 more
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Puromycin reactivity does not accurately localize translation at the subcellular level
eLife, 2020 Puromycin is a tyrosyl-tRNA mimic that blocks translation by labeling and releasing elongating polypeptide chains from translating ribosomes. Puromycin has been used in molecular biology research for decades as a translation inhibitor. The development of
Syed Usman Enam +6 more
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Efficient isolation of mouse deletion mutant embryonic stem cells by CRISPR
STAR Protocols, 2022 Summary: Gene functions can be assessed in mouse embryonic stem (ES) cells and in mutant mice derived from mutant ES cells. Here, we describe an approach for efficient isolation of the ES clones carrying deletion mutations at the target genes by CRISPR ...
Yuhan Liu +5 more
doaj +1 more source
Premature translational termination products are rapidly degraded substrates for MHC class I presentation. [PDF]
PLoS ONE, 2012 Nearly thirty percent of all newly synthesized polypeptides are targeted for rapid proteasome-mediated degradation. These rapidly degraded polypeptides (RDPs) are a source of antigenic substrates for the MHC class I presentation pathway, allowing for ...
Joshua R Lacsina +5 more
doaj +1 more source