Results 11 to 20 of about 126,114 (304)
Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish
Animal Cells and Systems, 2023 Puromycin treatment can cause glomerular injury to the kidney, leading to proteinuria. However, the pathogenesis of acute kidney injury and subsequent regeneration after puromycin administration in animal models remain unclear.
Soonil Koun +5 more
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Scientific Reports, 2021 Puromycin and the Streptomyces alboniger-derived puromycin N-acetyltransferase (PAC) enzyme form a commonly used system for selecting stably transfected cultured cells.
Alessandro T. Caputo +8 more
doaj +2 more sources
Effects of tacrolimus on autophagy protein LC3 in puromycin-damaged mouse podocytes
Journal of International Medical Research, 2020 Objective To investigate the mechanism through which tacrolimus, often used to treat refractory nephropathy, protects against puromycin-induced podocyte injury.
Li Yu, Guosheng Liu, Li Yu
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Bifunctional Protein Conferring Enhanced Green Fluorescence and Puromycin Resistance
BioTechniques, 2001 A new genetic marker was created in which sequences from enhanced green fluorescent protein were fused to those of puromycin N-acetyl transferase. The resulting fusion protein (EGFP-puro) conferred both green fluorescence and resistance to puromycin when
J. Abbate +4 more
doaj +2 more sources
The science of puromycin: From studies of ribosome function to applications in biotechnology
Computational and Structural Biotechnology Journal, 2020 Puromycin is a naturally occurring aminonucleoside antibiotic that inhibits protein synthesis by ribosome-catalyzed incorporation into the C-terminus of elongating nascent chains, blocking further extension and resulting in premature termination of ...
Ranen Aviner
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Investigation of protein synthesis in Drosophila larvae using puromycin labelling
Biology Open, 2017 Translational control of gene expression is an important regulator of growth, homeostasis and aging in Drosophila. The ability to measure changes in protein synthesis in response to genetic and environmental cues is therefore important in studying these ...
Lisa P. Deliu +2 more
doaj +3 more sources
Scientific ReportsRadiolabelled puromycin analogues will allow the quantification of protein synthesis through nuclear medicine-based imaging. A particularly useful application could be the non-invasive longitudinal visualisation of mycobacterial activity through direct ...
Sebastian Eigner +5 more
doaj +2 more sources
Rapid cell type-specific nascent proteome labeling in Drosophila
eLife, 2023 Controlled protein synthesis is required to regulate gene expression and is often carried out in a cell type-specific manner. Protein synthesis is commonly measured by labeling the nascent proteome with amino acid analogs or isotope-containing amino ...
Stefanny Villalobos-Cantor +6 more
doaj +1 more source
The Mechanism of Resistance to Puromycin and to the Puromycin-precursor O-Demethyl-puromycin in Streptomyces alboniger [PDF]
Microbiology, 1985 Ribosomes from Streptomyces alboniger are sensitive in vitro to puromycin and, to a lesser extent, to the puromycin-precursor O-demethyl-puromycin. The puromycin-inactivating enzyme (puromycin N-acetyltransferase) from S. alboniger also N-acetylates O-demethyl-puromycin.
J A, Pérez-González +2 more
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eLife, 2020 Puromycin is an amino-acyl transfer RNA analog widely employed in studies of protein synthesis. Since puromycin is covalently incorporated into nascent polypeptide chains, anti-puromycin immunofluorescence enables visualization of nascent protein ...
Benjamin D Hobson +4 more
doaj +1 more source