Results 11 to 20 of about 122,814 (265)

Puromycin-based purification of cells with high expression of the cytochrome P450 CYP3A4 gene from a patient with drug-induced liver injury (DILI)

Stem Cell Research & Therapy, 2022
Background Many drugs have the potential to induce the expression of drug-metabolizing enzymes, particularly cytochrome P450 3A4 (CYP3A4), in hepatocytes.
Shoko Miyata, Noriaki Saku, Saeko Akiyama, Palaksha Kanive Javaregowda, Kenta Ite, Nagisa Takashima, Masashi Toyoda, Kei Yura, Tohru Kimura, Hiroshi Nishina, Atsuko Nakazawa, Mureo Kasahara, Hidenori Nonaka, Tohru Kiyono, Akihiro Umezawa   +14 more
doaj   +1 more source

Nourseothricin N-acetyl transferase: a positive selection marker for mammalian cells. [PDF]

PLoS ONE, 2013
Development of Nourseothricin N-acetyl transferase (NAT) as a selection marker for mammalian cells is described. Mammalian cells are acutely susceptible to Nourseothricin, similar to the widely used drug Puromycin, and NAT allows for quick and robust ...
Bose S Kochupurakkal, J Dirk Iglehart
doaj   +1 more source

Easy and Rapid Binding Assay for Functional Analysis of Disulfide-Containing Peptides by a Pull-Down Method Using a Puromycin-Linker and a Cell-Free Translation System

Biology, 2015
Constrained peptides are an attractive class as affinity reagents or drug leads owing to their excellent binding properties. Many kinds of these peptides, such as cyclic peptides containing disulfide bridges, are found in nature or designed artificially ...
Yutaro Tanemura, Yuki Mochizuki, Shigefumi Kumachi, Naoto Nemoto   +3 more
doaj   +1 more source

Effect of Puromycin Analogues and Other Agents on Peptidyl-Puromycin Synthesis on Polyribosomes [PDF]

Antimicrobial Agents and Chemotherapy, 1973
The incorporation of [ 3 H]puromycin into nascent polypeptide chains of polyribosomes has proved to be a sensitive method of evaluating effects of inhibitors on peptide bond synthesis. Several analogues of puromycin were found to react with polyribosomes from both bacteria and rat liver. The
S, Pestka, R, Vince, S, Daluge, R, Harris   +3 more
openaire   +2 more sources

Antibiotics and RNase P

Antibiotics, 2016
RNase P is an essential endonuclease in tRNA biogenesis, which generates the mature 5′-termini of tRNAs. Most forms of RNase P are ribonucleoproteins, i.e., they consist of an essential RNA and protein subunits.
Denis Drainas
doaj   +1 more source

An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols

Molecules, 2000
We describe an improved synthetic strategy for the preparation of nucleic acid encoded peptide and protein libraries. A solid-phase format was used to prepare and purify a novel type of mRNA-template for in vitro mRNA-protein fusion synthesis.
Peter A. Lohse, Guannan Kuang, Markus Kurz   +2 more
doaj   +1 more source

Adrenalectomy and the Suppression of Memory by Puromycin [PDF]

Proceedings of the National Academy of Sciences, 1970
It has previously been shown that expression of memory of maze-learning in mice is blocked by puromycin injected intracerebrally one or more days after the training experience. Bilateral adrenalectomy before training has now been found to protect memory against the effects of puromycin. This protection is absent when adrenalectomy follows training.
J B, Flexner, L B, Flexner
openaire   +2 more sources

Targeting of herpes simplex virus 1 thymidine kinase gene sequences into the OCT4 locus of human induced pluripotent stem cells. [PDF]

PLoS ONE, 2013
The in vitro differentiation of human induced pluripotent stem cells (hiPSC) to generate specific types of cells is inefficient, and the remaining undifferentiated cells may form teratomas.
Wu Ou, Pingjuan Li, Jakob Reiser
doaj   +1 more source

Construction of Fosmid-based SARS-CoV-2 replicons for antiviral drug screening and replication analyses in biosafety level 2 facilities

Virus Research, 2023
The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has necessitated the global development of countermeasures since its outbreak.
Shunta Takazawa, Tomohiro Kotaki, Satsuki Nakamura, Chie Utsubo, Masanori Kameoka   +4 more
doaj   +1 more source

Induction of P-glycoprotein overexpression in brain endothelial cells as a model to study blood-brain barrier efflux transport

Frontiers in Drug Delivery
The blood-brain barrier (BBB) is comprised of specialized brain endothelial cells (BECs) that contribute to maintaining central nervous system (CNS) homeostasis.
Sarah F. Hathcock, Hallie E. Knight, Emma G. Tong, Alexandra E. Meyer, Henry D. Mauser, Nadine Vollmuth, Brandon J. Kim, Brandon J. Kim, Brandon J. Kim, Brandon J. Kim   +9 more
doaj   +1 more source