Results 1 to 10 of about 122,814 (265)
The science of puromycin: From studies of ribosome function to applications in biotechnology
Computational and Structural Biotechnology Journal, 2020 Puromycin is a naturally occurring aminonucleoside antibiotic that inhibits protein synthesis by ribosome-catalyzed incorporation into the C-terminus of elongating nascent chains, blocking further extension and resulting in premature termination of ...
Ranen Aviner
exaly +3 more sources
Polymergebundenes Puromycin / Resin Linked Puromycin
Zeitschrift Fur Naturforschung - Section C Journal of Biosciences, 1975 Abstract A biospecific resin for the retardation of ribosomal pep-tidyltransferase by affinity chromatography was prepared by condensing the α-amino group of puromycin with the N-hydroxysuccinimide ester of CH-Sepharose 4B to yield the resin-linked puromycin derivative 4.
Frank Seela
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Rapid cell type-specific nascent proteome labeling in Drosophila
eLife, 2023 Controlled protein synthesis is required to regulate gene expression and is often carried out in a cell type-specific manner. Protein synthesis is commonly measured by labeling the nascent proteome with amino acid analogs or isotope-containing amino ...
Stefanny Villalobos-Cantor +6 more
doaj +1 more source
Puromycin-induced kidney injury and subsequent regeneration in adult zebrafish
Animal Cells and Systems, 2023 Puromycin treatment can cause glomerular injury to the kidney, leading to proteinuria. However, the pathogenesis of acute kidney injury and subsequent regeneration after puromycin administration in animal models remain unclear.
Soonil Koun +5 more
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The Mechanism of Resistance to Puromycin and to the Puromycin-precursor O-Demethyl-puromycin in Streptomyces alboniger [PDF]
Microbiology, 1985 Ribosomes from Streptomyces alboniger are sensitive in vitro to puromycin and, to a lesser extent, to the puromycin-precursor O-demethyl-puromycin. The puromycin-inactivating enzyme (puromycin N-acetyltransferase) from S. alboniger also N-acetylates O-demethyl-puromycin.
J A, Pérez-González +2 more
openaire +2 more sources
eLife, 2020 Puromycin is an amino-acyl transfer RNA analog widely employed in studies of protein synthesis. Since puromycin is covalently incorporated into nascent polypeptide chains, anti-puromycin immunofluorescence enables visualization of nascent protein ...
Benjamin D Hobson +4 more
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Puromycin reactivity does not accurately localize translation at the subcellular level
eLife, 2020 Puromycin is a tyrosyl-tRNA mimic that blocks translation by labeling and releasing elongating polypeptide chains from translating ribosomes. Puromycin has been used in molecular biology research for decades as a translation inhibitor. The development of
Syed Usman Enam +6 more
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Efficient isolation of mouse deletion mutant embryonic stem cells by CRISPR
STAR Protocols, 2022 Summary: Gene functions can be assessed in mouse embryonic stem (ES) cells and in mutant mice derived from mutant ES cells. Here, we describe an approach for efficient isolation of the ES clones carrying deletion mutations at the target genes by CRISPR ...
Yuhan Liu +5 more
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Premature translational termination products are rapidly degraded substrates for MHC class I presentation. [PDF]
PLoS ONE, 2012 Nearly thirty percent of all newly synthesized polypeptides are targeted for rapid proteasome-mediated degradation. These rapidly degraded polypeptides (RDPs) are a source of antigenic substrates for the MHC class I presentation pathway, allowing for ...
Joshua R Lacsina +5 more
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Effects of tacrolimus on autophagy protein LC3 in puromycin-damaged mouse podocytes
Journal of International Medical Research, 2020 Objective To investigate the mechanism through which tacrolimus, often used to treat refractory nephropathy, protects against puromycin-induced podocyte injury.
Xiao-qing Yang +6 more
doaj +1 more source