Results 21 to 30 of about 126,114 (304)

Puromycin reactivity does not accurately localize translation at the subcellular level

eLife, 2020
Puromycin is a tyrosyl-tRNA mimic that blocks translation by labeling and releasing elongating polypeptide chains from translating ribosomes. Puromycin has been used in molecular biology research for decades as a translation inhibitor. The development of
Syed Usman Enam, Boris Zinshteyn, Daniel H Goldman, Madeline Cassani, Nathan M Livingston, Geraldine Seydoux, Rachel Green   +6 more
doaj   +1 more source

Efficient isolation of mouse deletion mutant embryonic stem cells by CRISPR

STAR Protocols, 2022
Summary: Gene functions can be assessed in mouse embryonic stem (ES) cells and in mutant mice derived from mutant ES cells. Here, we describe an approach for efficient isolation of the ES clones carrying deletion mutations at the target genes by CRISPR ...
Yuhan Liu, Qian Chen, Chenglin Song, Zhen Xu, Shuting Yang, Xiajun Li   +5 more
doaj   +1 more source

Complete puromycin incorporation western blot data.

, 2023
(a) Comparison of puromycin incorporation in mock-inoculated plants in 2 experimental repeats (b). Quantification of puromycin signal intensity from images shown in (a).
Jean-Luc Gallois (3142977), Christophe Robaglia (75937), Delyan Zafirov (17420106), Julia Novion Ducassou (10919549), Karen S. Browning (1874980), Nathalie Giovinazzo (17420109), Cécile Lecampion (75936), Ben Field (5132546), Yohann Couté (505302)   +8 more
core   +1 more source

Premature translational termination products are rapidly degraded substrates for MHC class I presentation. [PDF]

PLoS ONE, 2012
Nearly thirty percent of all newly synthesized polypeptides are targeted for rapid proteasome-mediated degradation. These rapidly degraded polypeptides (RDPs) are a source of antigenic substrates for the MHC class I presentation pathway, allowing for ...
Joshua R Lacsina, Odessa A Marks, Xiongfei Liu, David W Reid, Sujatha Jagannathan, Christopher V Nicchitta   +5 more
doaj   +1 more source

Puromycin-based purification of cells with high expression of the cytochrome P450 CYP3A4 gene from a patient with drug-induced liver injury (DILI)

Stem Cell Research & Therapy, 2022
Background Many drugs have the potential to induce the expression of drug-metabolizing enzymes, particularly cytochrome P450 3A4 (CYP3A4), in hepatocytes.
Shoko Miyata, Noriaki Saku, Saeko Akiyama, Palaksha Kanive Javaregowda, Kenta Ite, Nagisa Takashima, Masashi Toyoda, Kei Yura, Tohru Kimura, Hiroshi Nishina, Atsuko Nakazawa, Mureo Kasahara, Hidenori Nonaka, Tohru Kiyono, Akihiro Umezawa   +14 more
doaj   +1 more source

Modeling puromycin interaction with a closed form of PSA.

, 2023
The closed form model for PSA was constructed by superimposing the open PSA crystal structure reported here on the closed form AlphaFold model domain by domain.
K. Martin Chow (772610), Louis B. Hersh (15852915), Eun Suk Song (16541911), David W. Rodgers (7406882), Sowmya Madabushi (16541908), Anwesha Goswami (2023816)   +5 more
core   +1 more source

Nourseothricin N-acetyl transferase: a positive selection marker for mammalian cells. [PDF]

PLoS ONE, 2013
Development of Nourseothricin N-acetyl transferase (NAT) as a selection marker for mammalian cells is described. Mammalian cells are acutely susceptible to Nourseothricin, similar to the widely used drug Puromycin, and NAT allows for quick and robust ...
Bose S Kochupurakkal, J Dirk Iglehart
doaj   +1 more source

Cell growth of puromycin control HCT116^ATRX-/- clones.

, 2022
Growth curve displaying PDs against days in culture for the puromycin control clones (n = 12) that were transfected with a puromycin selection gene to query the effects of a viral integration on HCT116ATRX-/- cells survival. (DOCX)
Duncan M. Baird (14094318), Eric A. Hendrickson (14094315), Julia W. Grimstead (3897586), Rhiannon E. Jones (14094309), Adam Harvey (485074), Helene E. B. Geiller (14094306), Kez Cleal (14094312)   +6 more
core   +1 more source

Easy and Rapid Binding Assay for Functional Analysis of Disulfide-Containing Peptides by a Pull-Down Method Using a Puromycin-Linker and a Cell-Free Translation System

Biology, 2015
Constrained peptides are an attractive class as affinity reagents or drug leads owing to their excellent binding properties. Many kinds of these peptides, such as cyclic peptides containing disulfide bridges, are found in nature or designed artificially ...
Yutaro Tanemura, Yuki Mochizuki, Shigefumi Kumachi, Naoto Nemoto   +3 more
doaj   +1 more source

Effect of Puromycin Analogues and Other Agents on Peptidyl-Puromycin Synthesis on Polyribosomes [PDF]

Antimicrobial Agents and Chemotherapy, 1973
The incorporation of [ 3 H]puromycin into nascent polypeptide chains of polyribosomes has proved to be a sensitive method of evaluating effects of inhibitors on peptide bond synthesis. Several analogues of puromycin were found to react with polyribosomes from both bacteria and rat liver. The
S, Pestka, R, Vince, S, Daluge, R, Harris   +3 more
openaire   +2 more sources