Results 21 to 30 of about 122,814 (265)

A Microplate-Based Nonradioactive Protein Synthesis Assay: Application to TRAIL Sensitization by Protein Synthesis Inhibitors.

PLoS ONE, 2016
Non-radioactive assays based on incorporation of puromycin into newly synthesized proteins and subsequent detection using anti-puromycin antibodies have been previously reported and well-validated.
Curtis J Henrich
doaj   +1 more source

Structure-guided selection of puromycin N-acetyltransferase mutants with enhanced selection stringency for deriving mammalian cell lines expressing recombinant proteins

Scientific Reports, 2021
Puromycin and the Streptomyces alboniger-derived puromycin N-acetyltransferase (PAC) enzyme form a commonly used system for selecting stably transfected cultured cells.
Alessandro T. Caputo, Oliver M. Eder, Hana Bereznakova, Heleen Pothuis, Albert Ardevol, Janet Newman, Stewart Nuttall, Thomas S. Peat, Timothy E. Adams   +8 more
doaj   +1 more source

Protocol for Efficient Protein Synthesis Detection by Click Chemistry in Colorectal Cancer Patient-Derived Organoids Grown In Vitro

STAR Protocols, 2020
Summary: Here, we describe a protocol to detect and visualize protein synthesis by click-chemistry-based immunofluorescence in patient-derived organoids (PDOs) in vitro.
Clara Morral, Jelena Stanisavljevic, Eduard Batlle   +2 more
doaj   +1 more source

Protective effects of rituximab on puromycin-induced apoptosis, loss of adhesion and cytoskeletal alterations in human podocytes

Scientific Reports, 2022
Podocytes are highly specialized cells playing a key role in the filtration function of the kidney. A damaged podocyte ultrastructure is associated with a reorganization of the actin cytoskeleton and accompanied with a loss of adhesion to the glomerular ...
Stefanie Jeruschke, Dana Alex, Peter Friedrich Hoyer, Stefanie Weber   +3 more
doaj   +1 more source

Protocol for assessing translation in living Drosophila imaginal discs by O-propargyl-puromycin incorporation

STAR Protocols, 2023
Summary: Translation is a fundamental process of cellular behavior. Here, we present a protocol for measuring translation in Drosophila epithelial tissues using O-propargyl-puromycin (OPP), a puromycin derivative.
Marianthi Kiparaki, Nicholas E. Baker
doaj   +1 more source

Quantitative IC₅₀ Analysis of Puromycin-Induced Cytotoxicity in NIH/3T3 Cells Using a Multi-Well Array Impedance Biosensor

Biosensors
ECIS-based impedance biosensors have been extensively studied in various fields including cancer research, microbiology, and immunology. However, most studies have primarily focused on monitoring cellular behavior through impedance changes, with ...
Seok-kyu Kim, SuGwon Nam, Moongyu Jang
doaj   +1 more source

Bifunctional Protein Conferring Enhanced Green Fluorescence and Puromycin Resistance

BioTechniques, 2001
A new genetic marker was created in which sequences from enhanced green fluorescent protein were fused to those of puromycin N-acetyl transferase. The resulting fusion protein (EGFP-puro) conferred both green fluorescence and resistance to puromycin when
J. Abbate, J.C. Lacayo, M. Prichard, G. Pari, M.A. McVoy   +4 more
doaj   +1 more source

Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling

STAR Protocols, 2022
Summary: Translational regulation is a fundamental step in gene expression with critical roles in biological processes within a cell. Here, we describe a protocol to assess translation activity in mammalian cells by incorporation of O-propargyl-puromycin
Jack Chun-Chieh Hsu, Joanna B. Pawlak, Maudry Laurent-Rolle, Peter Cresswell   +3 more
doaj   +1 more source

Imaging of protein synthesis with puromycin [PDF]

Proceedings of the National Academy of Sciences, 2012
With great interest, we read the work by Liu et al. (1), which described the development of an alkyne analog of puromycin for labeling and imaging of newly synthesized peptides. Available techniques for this type of measurement are very limited, and the work by Liu et al.
Craig A, Goodman, Philippe, Pierre, Troy A, Hornberger   +2 more
openaire   +2 more sources

Investigation of protein synthesis in Drosophila larvae using puromycin labelling

Biology Open, 2017
Translational control of gene expression is an important regulator of growth, homeostasis and aging in Drosophila. The ability to measure changes in protein synthesis in response to genetic and environmental cues is therefore important in studying these ...
Lisa P. Deliu, Abhishek Ghosh, Savraj S. Grewal   +2 more
doaj   +1 more source