Results 41 to 50 of about 122,814 (265)

Puromycin inhibition of cholesterol absorption in the rat

Journal of Lipid Research, 1977
The effect of puromycin on the intestinal absorption of cholesterol has been studied in rats with indwelling catheters in the left thoracic lymphatic duct.
G V Vahouny, M Ito, E M Blendermann, L L Gallo, C R Treadwell   +4 more
doaj   +1 more source

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

Puromycin-sensitive Aminopeptidase [PDF]

Journal of Biological Chemistry, 1995
The present invention relates to puromycin-sensitive aminopeptidases, antibodies generated against said aminopeptidases, and to means and methods for the production thereof. The invention is also directed to nucleic acids coding for said aminopeptidases, and fragments thereof, to methods of obtaining such nucleic acid molecules or fragments of the ...
Daniel B. Constam, Andreas R. Tobler, Anne Rensing-Ehl, Iris Kemler, Louis B. Hersh, Adriano Fontana   +5 more
openaire   +1 more source

ZW4864‐mediated inhibition of the β‐catenin/BCL9/BCL9L complex reveals therapeutic potential in bladder cancer

Molecular Oncology, EarlyView.
BCL9 and BCL9L drive bladder cancer progression by enhancing β‐catenin signaling, promoting proliferation, migration, invasion, and organoid growth. Genetic depletion of BCL9(L) suppresses malignant phenotypes, while pharmacological disruption of the β‐catenin/BCL9(L) complex with ZW4864 inhibits canonical Wnt signaling and tumor‐associated cellular ...
Roland Kotolloshi, Mandy Berndt‐Paetz, Eileen Lerner, Gregoire Najjar, Anca Azoitei, Krishna Pal Singh, Shailendra Kumar Gupta, Olaf Wolkenhauer, Cagatay Günes, Otmar Huber, Marc‐Oliver Grimm, Daniel Steinbach   +11 more
wiley   +1 more source

Visualisation of in vivo protein synthesis during mycobacterial infection through [68Ga]Ga-DOTA-puromycin µPET/MRI

Scientific Reports
Radiolabelled puromycin analogues will allow the quantification of protein synthesis through nuclear medicine-based imaging. A particularly useful application could be the non-invasive longitudinal visualisation of mycobacterial activity through direct ...
Sebastian Eigner, Janke Kleynhans, Dennis R. Beckford Vera, Mike M. Sathekge, Katerina Eigner Henke, Thomas Ebenhan   +5 more
doaj   +1 more source

Attachment of Proteolytic Enzyme Inhibitors to Vascular Prosthesis—An Analysis of Binding and Antimicrobial Properties

Molecules
Prosthetic infections are associated with high morbidity, mortality, and relapse rates, making them still a serious problem for implantology. Staphylococcus aureus is one of the most common bacterial pathogens causing prosthetic infections.
Aleksandra Mordzińska-Rak, Katarzyna Szałapata, Jerzy Wydrych, Mariusz Gagoś, Anna Jarosz-Wilkołazka   +4 more
doaj   +1 more source

Inactivation of an integrated antibiotic resistance gene in mammalian cells to re-enable antibiotic selection

BioTechniques, 2014
Removing an antibiotic resistance gene allows the same antibiotic to be re-used in the next round of genetic manipulation. Here we applied the CRISPR/Cas system to disrupt the puromycin resistance gene in an engineered mouse embryonic stem cell line and ...
Peiling Ni, Qian Zhang, Haixia Chen, Lingyi Chen   +3 more
doaj   +1 more source

Somatostatin receptor 4 (SSTR4) is a tumor suppressor in cutaneous and head & neck squamous cell carcinomas

Molecular Oncology, EarlyView.
This study identifies somatostatin receptor 4 (Sstr4) as a critical tumor suppressor against skin and head/neck cancers (HNSCC, cSCC, and BCC). The loss of Sstr4 removes a check on cell growth, causing hyperactivation of the MAPK‐ERK signaling pathway (↑).
Ali Taqvi, Tuan Khang Huynh, Adway Kadam, Zahra Kardan, Yue Yan, Amina Jamal Laham, Sampath Kumar Loganathan   +6 more
wiley   +1 more source

Real-Time Monitoring of NIH/3T3 Cell Growth and Drug Reaction Using Impedance Biosensors and Comparison with Biological Assays

Biosensors
Impedance biosensors are manufactured on glass slides using a semiconductor process to monitor cell growth and cell–drug reactions in real time, and the results are compared with biological assay results to confirm the validity of impedance measurement ...
Seok-kyu Kim, Gayoung Lee, Yeeun Kim, Dahyun Kang, Moongyu Jang   +4 more
doaj   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source