Results 41 to 50 of about 126,114 (304)

Confirmation of expression of SV40 and puromycin resistance gene.

, 2023
(a) Primer sequences used to analyze transgene expression, provided by ALSTEM, Inc. (b) PCR products to confirm transgene expression in AcoSV40 cells. Lane 1.
Erika Moore (16510212), Michele N. Dill (16510206), Mohammad Tabatabaei (16510209), Chelsey S. Simmons (4067950), Manasi Kamat (9561347), Kari B. Basso (9189020)   +5 more
core   +1 more source

Protocol for assessing translation in living Drosophila imaginal discs by O-propargyl-puromycin incorporation

STAR Protocols, 2023
Summary: Translation is a fundamental process of cellular behavior. Here, we present a protocol for measuring translation in Drosophila epithelial tissues using O-propargyl-puromycin (OPP), a puromycin derivative.
Marianthi Kiparaki, Nicholas E. Baker
doaj   +1 more source

Transient puromycin selection increases reporter KI efficiency.

, 2018
(A) Schematic of gene reporter design created by CRISPR editing. (B) Phase and fluorescence microscopy images of TBP-P2A-eGFP KI into H9 hESCs after puromycin selection. Scale bar = 100 μm.
Donald J. Zack (9715), Dennis S. Rice (304216), Valentin M. Sluch (6029387), Claire Wenger (6029390), Xitiz Chamling (390467), Yukan Duan (6029393)   +5 more
core   +1 more source

Protocol for Efficient Protein Synthesis Detection by Click Chemistry in Colorectal Cancer Patient-Derived Organoids Grown In Vitro

STAR Protocols, 2020
Summary: Here, we describe a protocol to detect and visualize protein synthesis by click-chemistry-based immunofluorescence in patient-derived organoids (PDOs) in vitro.
Clara Morral, Jelena Stanisavljevic, Eduard Batlle   +2 more
doaj   +1 more source

Protective effects of rituximab on puromycin-induced apoptosis, loss of adhesion and cytoskeletal alterations in human podocytes

Scientific Reports, 2022
Podocytes are highly specialized cells playing a key role in the filtration function of the kidney. A damaged podocyte ultrastructure is associated with a reorganization of the actin cytoskeleton and accompanied with a loss of adhesion to the glomerular ...
Stefanie Jeruschke, Dana Alex, Peter Friedrich Hoyer, Stefanie Weber   +3 more
doaj   +1 more source

Quantitative IC₅₀ Analysis of Puromycin-Induced Cytotoxicity in NIH/3T3 Cells Using a Multi-Well Array Impedance Biosensor

Biosensors
ECIS-based impedance biosensors have been extensively studied in various fields including cancer research, microbiology, and immunology. However, most studies have primarily focused on monitoring cellular behavior through impedance changes, with ...
Seok-kyu Kim, SuGwon Nam, Moongyu Jang
doaj   +1 more source

Parthenogenetic activation of human oocytes by puromycin

, 1992
Objective: Our objective was to obtain parthenogenetic activation of unfertilized human oocytes by puromycin and to try to use this procedure for cytogenetic purposes. Setting: The setting was our IVF laboratory.
Cieslak, J, Dyban, A, Wolf, G, De Sutter, Petra, Verlinsky, Y, Dozortsev, D   +5 more
core   +1 more source

Screening of AAK1 interaction proteins and its role in regulating global translation level in cells

Shanghai Jiaotong Daxue xuebao. Yixue ban, 2023
Objective·To investigate noval interacting partners for adaptor-associated protein kinase 1 (AAK1) and AAK1-mediated biological functions besides clathrin-mediated endocytosis.Methods·The labeled AAK1 vector and the blank control vector were transfected ...
JIANG Guixian, HU Ronggui, WU Hao
doaj   +1 more source

Imaging of protein synthesis with puromycin [PDF]

Proceedings of the National Academy of Sciences, 2012
With great interest, we read the work by Liu et al. (1), which described the development of an alkyne analog of puromycin for labeling and imaging of newly synthesized peptides. Available techniques for this type of measurement are very limited, and the work by Liu et al.
Craig A, Goodman, Philippe, Pierre, Troy A, Hornberger   +2 more
openaire   +2 more sources

Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling

STAR Protocols, 2022
Summary: Translational regulation is a fundamental step in gene expression with critical roles in biological processes within a cell. Here, we describe a protocol to assess translation activity in mammalian cells by incorporation of O-propargyl-puromycin
Jack Chun-Chieh Hsu, Joanna B. Pawlak, Maudry Laurent-Rolle, Peter Cresswell   +3 more
doaj   +1 more source