Results 61 to 70 of about 126,114 (304)

Polymergebundenes Puromycin / Resin Linked Puromycin

Zeitschrift für Naturforschung C, 1975
Abstract A biospecific resin for the retardation of ribosomal pep-tidyltransferase by affinity chromatography was prepared by condensing the α-amino group of puromycin with the N-hydroxysuccinimide ester of CH-Sepharose 4B to yield the resin-linked puromycin derivative 4.
openaire   +1 more source

Proteasome inhibitor, ixazomib prevents topoisomerase‐I degradation and reverses irinotecan resistance in colorectal cancer

Molecular Oncology, EarlyView.
Ixazomib inhibits proteasome‐mediated degradation of topoisomerase I induced by irinotecan, thereby restoring drug sensitivity and promoting tumor cell death in colorectal cancer. Irinotecan, a topoisomerase I (topoI) inhibitor, is widely used for colorectal cancer, but resistance remains a major clinical challenge.
Yuho Ebata, Koji Ando, Hirofumi Hasuda, Koshi Mimori, Elizabeth C. Unan, Siddhartha Pulukuri, Aahana Tiku, Allison Berger, Eiji Oki, Ajit Bharti, Tomoharu Yoshizumi   +10 more
wiley   +1 more source

Puromycin-based vectors promote a ROS-dependent recruitment of PML to nuclear inclusions enriched with HSP70 and Proteasomes

BMC Cell Biology, 2009
Background Promyelocytic Leukemia (PML) protein can interact with a multitude of cellular factors and has been implicated in the regulation of various processes, including protein sequestration, cell cycle regulation and DNA damage responses.
Shen Hong, Moran Diarmuid M, Maki Carl G
doaj   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

Molecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober, Leire Moreno‐Cugnon, Laura Martínez‐Pérez, Amaia Ercilla, Amaia Zabala‐Letona, Adrián Vicente‐Barrueco, Maider Fagoaga‐Eugui, Saioa Garcia‐Longarte, Blanca Calle‐Ciborro, Encarnación Pérez‐Andrés, Onintza Carlevaris, Sara Pozo, Isabel Mendizabal, Ugo Mayor, Arkaitz Carracedo, Violaine Sée, Edurne Berra   +16 more
wiley   +1 more source

Puromycin-sensitive Aminopeptidase [PDF]

Journal of Biological Chemistry, 1995
The present invention relates to puromycin-sensitive aminopeptidases, antibodies generated against said aminopeptidases, and to means and methods for the production thereof. The invention is also directed to nucleic acids coding for said aminopeptidases, and fragments thereof, to methods of obtaining such nucleic acid molecules or fragments of the ...
Daniel B. Constam, Andreas R. Tobler, Anne Rensing-Ehl, Iris Kemler, Louis B. Hersh, Adriano Fontana   +5 more
openaire   +1 more source

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

Attachment of Proteolytic Enzyme Inhibitors to Vascular Prosthesis—An Analysis of Binding and Antimicrobial Properties

Molecules
Prosthetic infections are associated with high morbidity, mortality, and relapse rates, making them still a serious problem for implantology. Staphylococcus aureus is one of the most common bacterial pathogens causing prosthetic infections.
Aleksandra Mordzińska-Rak, Katarzyna Szałapata, Jerzy Wydrych, Mariusz Gagoś, Anna Jarosz-Wilkołazka   +4 more
doaj   +1 more source

Inactivation of an integrated antibiotic resistance gene in mammalian cells to re-enable antibiotic selection

BioTechniques, 2014
Removing an antibiotic resistance gene allows the same antibiotic to be re-used in the next round of genetic manipulation. Here we applied the CRISPR/Cas system to disrupt the puromycin resistance gene in an engineered mouse embryonic stem cell line and ...
Peiling Ni, Qian Zhang, Haixia Chen, Lingyi Chen   +3 more
doaj   +1 more source

Puromycin reaction for the A site-bound peptidyl-tRNA

, 1992
AcPhe2-tRNAPhe synthesized in 70S ribosomes after consecutive binding of AcPhe-tRNAPhe at the P sites and EF-Tu-directed binding of Phe-tRNAPhe at the A sites is able to react quantitatively with puromycin in the absence of EF-G.
Kirillov, S., Shapkina, T., V. Makhno, Semenkov, Yu., T. Shapkina, Makhno, V., Yu. Semenkov, S. Kirillov   +7 more
core   +1 more source