Results 71 to 80 of about 126,114 (304)

Real-Time Monitoring of NIH/3T3 Cell Growth and Drug Reaction Using Impedance Biosensors and Comparison with Biological Assays

Biosensors
Impedance biosensors are manufactured on glass slides using a semiconductor process to monitor cell growth and cell–drug reactions in real time, and the results are compared with biological assay results to confirm the validity of impedance measurement ...
Seok-kyu Kim, Gayoung Lee, Yeeun Kim, Dahyun Kang, Moongyu Jang   +4 more
doaj   +1 more source

ZW4864‐mediated inhibition of the β‐catenin/BCL9/BCL9L complex reveals therapeutic potential in bladder cancer

Molecular Oncology, EarlyView.
BCL9 and BCL9L drive bladder cancer progression by enhancing β‐catenin signaling, promoting proliferation, migration, invasion, and organoid growth. Genetic depletion of BCL9(L) suppresses malignant phenotypes, while pharmacological disruption of the β‐catenin/BCL9(L) complex with ZW4864 inhibits canonical Wnt signaling and tumor‐associated cellular ...
Roland Kotolloshi, Mandy Berndt‐Paetz, Eileen Lerner, Gregoire Najjar, Anca Azoitei, Krishna Pal Singh, Shailendra Kumar Gupta, Olaf Wolkenhauer, Cagatay Günes, Otmar Huber, Marc‐Oliver Grimm, Daniel Steinbach   +11 more
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Puromycin oligonucleotides reveal steric restrictions for ribosome entry and multiple modes of translation inhibition

, 2002
Peptidyl transferase inhibitors have generally been studied using simple systems and remain largely unexamined in in vitro translation extracts. Here, we investigate the potency, product distribution, and mechanism of various puromycin–oligonucleotide ...
Roberts, Richard W., RICHARD W. ROBERTS, SHELLEY R. STARCK, Starck, Shelley R.   +3 more
core   +1 more source

Differential transport of cholesterol and oleic acid in lymph lipoproteins: sex differences in puromycin sensitivity

Journal of Lipid Research, 1980
Adult rats of both sexes were prepared with indwelling drainage catheters in the left thoracic lymphatic duct, and with duodenal infusion catheters. Control and puromycin-treated animals were administered an aqueous test emulsion containing [7alpha-(3)H ...
G V Vahouny, E M Blendermann, L L Gallo, C R Treadwell   +3 more
doaj   +1 more source

Primary transgenic bovine cells and their rejuvenated cloned equivalents show transgene-specific epigenetic differences. [PDF]

PLoS ONE, 2012
Cell-mediated transgenesis, based on somatic cell nuclear transfer (SCNT), provides the opportunity to shape the genetic make-up of cattle. Bovine primary fetal fibroblasts, commonly used cells for SCNT, have a limited lifespan, and complex genetic ...
Lucia Alonso-González, Christine Couldrey, Marcus W Meinhardt, Sally A Cole, David N Wells, Götz Laible   +5 more
doaj   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source

Loss of REDD1 prevents chemotherapy‐induced muscle atrophy and weakness in mice

Journal of Cachexia, Sarcopenia and Muscle, 2021
Background Chemotherapy is an essential treatment to combat solid tumours and mitigate metastasis. Chemotherapy causes side effects including muscle wasting and weakness.
Brian A. Hain, Haifang Xu, David L. Waning   +2 more
doaj   +1 more source

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Species-specific differences in the toxicity of puromycin towards cultured human and Chinese hamster cells

, 1988
The toxicity of the protein synthesis inhibitor puromycin towards a number of human and Chinese hamster cell lines has been examined. In comparison to cells of human origin, Chinese hamster cells exhibited about 25-fold higher resistance towards ...
Anil K. Dudani, Rajni Gupta, Dudani, Anil K., Radhey S. Gupta, Gupta, Radhey S., Gupta, Rajni   +5 more
core   +1 more source