Effects of tacrolimus on autophagy protein LC3 in puromycin-damaged mouse podocytes
Journal of International Medical Research, 2020 Objective To investigate the mechanism through which tacrolimus, often used to treat refractory nephropathy, protects against puromycin-induced podocyte injury.
Xiao-qing Yang +6 more
doaj +1 more source
Investigating the functionality of an OCT4-short response element in human induced pluripotent stem cells. [PDF]
, 2016 Pluripotent stem cells offer great therapeutic promise for personalized treatment platforms for numerous injuries, disorders, and diseases. Octamer-binding transcription factor 4 (OCT4) is a key regulatory gene maintaining pluripotency and self-renewal ...
Awe, Jason P +9 more
core +3 more sources
Cytometric analysis, genetic manipulation and antibiotic selection of the snail embryonic cell line Bge from Biomphalaria glabrata, the intermediate host of Schistosoma mansoni. [PDF]
, 2015 The invertebrate cell line, Bge, from embryos of the snail Biomphalaria glabrata, remains to date the only established cell line from any species of the Phylum Mollusca.
Bridger, J +8 more
core +1 more source
The Mechanism of Resistance to Puromycin and to the Puromycin-precursor O-Demethyl-puromycin in Streptomyces alboniger [PDF]
Microbiology, 1985 Ribosomes from Streptomyces alboniger are sensitive in vitro to puromycin and, to a lesser extent, to the puromycin-precursor O-demethyl-puromycin. The puromycin-inactivating enzyme (puromycin N-acetyltransferase) from S. alboniger also N-acetylates O-demethyl-puromycin.
J A, Pérez-González +2 more
openaire +2 more sources
Stem Cell Research & Therapy, 2022 Background Many drugs have the potential to induce the expression of drug-metabolizing enzymes, particularly cytochrome P450 3A4 (CYP3A4), in hepatocytes.
Shoko Miyata +14 more
doaj +1 more source
Transport of Cytoplasmically Synthesized Proteins into the Mitochondria in a Cell Free System from Neurospora crassa [PDF]
, 1977 Synthesis and transport of mitochondrial proteins were followed in a cell-free homogenate of Neurospora crassa in which mitochondrial translation was inhibited. Proteins synthesized on cytoplasmic ribosomes are transferred into the mitochondrial fraction.
Andrews T. M. +30 more
core +1 more source
Puromycin Sensitivity of Ribosomal Label after Incorporation of 14C-Labelled Amino Acids into Isolated Mitochondria from Neurospora crassa [PDF]
, 1969 Radioactive amino acids were incorporated into isolated mitochondria from Neurospora crassa. Then the mitochondrial ribosomes were isolated and submitted to density gradient centrifugation. A preferential labelling of polysomes was observed.
Dingman C. W. +8 more
core +2 more sources
Nourseothricin N-acetyl transferase: a positive selection marker for mammalian cells. [PDF]
PLoS ONE, 2013 Development of Nourseothricin N-acetyl transferase (NAT) as a selection marker for mammalian cells is described. Mammalian cells are acutely susceptible to Nourseothricin, similar to the widely used drug Puromycin, and NAT allows for quick and robust ...
Bose S Kochupurakkal, J Dirk Iglehart
doaj +1 more source
Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia [PDF]
, 1971 Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-3H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide ...
Wilson, David L.
core +4 more sources
Puromycin-sensitive Aminopeptidase [PDF]
Journal of Biological Chemistry, 1995 The present invention relates to puromycin-sensitive aminopeptidases, antibodies generated against said aminopeptidases, and to means and methods for the production thereof. The invention is also directed to nucleic acids coding for said aminopeptidases, and fragments thereof, to methods of obtaining such nucleic acid molecules or fragments of the ...
Daniel B. Constam +5 more
openaire +1 more source