Results 31 to 40 of about 159,015 (286)

Construction of Fosmid-based SARS-CoV-2 replicons for antiviral drug screening and replication analyses in biosafety level 2 facilities

Virus Research, 2023
The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has necessitated the global development of countermeasures since its outbreak.
Shunta Takazawa, Tomohiro Kotaki, Satsuki Nakamura, Chie Utsubo, Masanori Kameoka   +4 more
doaj   +1 more source

CRISPR/Cas9-based editing of a sensitive transcriptional regulatory element to achieve cell type-specific knockdown of the NEMO scaffold protein [PDF]

, 2019
The use of alternative promoters for the cell type-specific expression of a given mRNA/protein is a common cell strategy. NEMO is a scaffold protein required for canonical NF-κB signaling.
Babaei, Milad, DiRusso, Christopher J., Gilmore, Thomas D., Kagermazova, Larisa, Liu, Yuekun, McCaslin, Ethan Z., Miyamoto, Shigeki, Wuerzberger-Davis, Shelly M., Yeo, Alan T.   +8 more
core   +1 more source

Induction of P-glycoprotein overexpression in brain endothelial cells as a model to study blood-brain barrier efflux transport

Frontiers in Drug Delivery
The blood-brain barrier (BBB) is comprised of specialized brain endothelial cells (BECs) that contribute to maintaining central nervous system (CNS) homeostasis.
Sarah F. Hathcock, Hallie E. Knight, Emma G. Tong, Alexandra E. Meyer, Henry D. Mauser, Nadine Vollmuth, Brandon J. Kim, Brandon J. Kim, Brandon J. Kim, Brandon J. Kim   +9 more
doaj   +1 more source

A Microplate-Based Nonradioactive Protein Synthesis Assay: Application to TRAIL Sensitization by Protein Synthesis Inhibitors.

PLoS ONE, 2016
Non-radioactive assays based on incorporation of puromycin into newly synthesized proteins and subsequent detection using anti-puromycin antibodies have been previously reported and well-validated.
Curtis J Henrich
doaj   +1 more source

HCV IRES manipulates the ribosome to promote the switch from translation initiation to elongation. [PDF]

, 2013
The internal ribosome entry site (IRES) of the hepatitis C virus (HCV) drives noncanonical initiation of protein synthesis necessary for viral replication.
A Ben-Shem, A Devaraj, AA Malygin, AD Petropoulos, AY Keel, Breanna S Vollmar, C Chen, CM Spahn, CS Fraser, CS Fraser, D Boehringer, D Hartz, D Sousa, Dan Shi, DD Ian'shina, DV Sizova, EF Pettersen, F Odreman-Macchioli, F Peske, F Robert, GA Otto, GJ Kleywegt, H Ji, IB Lomakin, IM Terenin, J Bukh, J Rabl, J Wower, JA Mindell, JE Wilson, Jeffrey S Kieft, JH Kim, JM Fringer, JR Lytle, JR Lytle, JS Kieft, JS Kieft, K Tsukiyama-Kohara, KE Berry, KI Kalliampakou, LA Passmore, M Honda, M Ohi, M Selmer, M Stoneley, M van Heel, ME Filbin, Megan E Filbin, MG Acker, MM Yusupov, N Grigorieff, N Locker, O Galkin, P Antúnez de Mayolo, P Simmonds, PJ Lukavsky, PJ Lukavsky, RE Monro, RJ Jackson, S Fukushi, S Uemura, SM Dibrov, T Döring, Tamir Gonen, TV Pestova, TV Pestova, U Geigenmüller, VG Kolupaeva, Y Yu   +68 more
core   +1 more source

Polymergebundenes Puromycin / Resin Linked Puromycin

Zeitschrift für Naturforschung C, 1975
Abstract A biospecific resin for the retardation of ribosomal pep-tidyltransferase by affinity chromatography was prepared by condensing the α-amino group of puromycin with the N-hydroxysuccinimide ester of CH-Sepharose 4B to yield the resin-linked puromycin derivative 4.
openaire   +1 more source

GSK3-mediated raptor phosphorylation supports amino acid-dependent Q2 mTORC1-directed signalling [PDF]

, 2015
The mammalian or mechanistic target of rapamycin (mTOR) complex 1 (mTORC1) is a ubiquitously expressed multimeric protein kinase complex that integrates nutrient and growth factor signals for the co-ordinated regulation of cellular metabolism and cell ...
Alan Prescott, Averous, Avruch, Azoulay-Alfaguter, Bain, Bar-Peled, Bar-Peled, Bradford, Carriere, Carriere, Clare Stretton, Coghlan, Cross, Demetriades, Dibble, Doble, Fonseca, Foster, Frey, Ganley, Garcia-Martinez, Gwinn, Gwinn, Hajduch, Hara, Harinder S. Hundal, Hosokawa, Ian G. Ganley, Inoki, Inoki, Inoki, Jewell, Jung, Kelleher, Kim, Kim, Kwak, Lipina, MacAulay, Marchand, Martina, Meijer, Menon, Michael J. Munson, Oshiro, Oshiro, Parr, Peter M. Taylor, Pfaffl, Ramirez-Valle, Rebsamen, Sancak, Shin, Smith, Stretton, Sutherland, Thorsten M. Hoffmann, Wang, Wang, Yoon, Zoncu   +60 more
core   +3 more sources

Protocol for Efficient Protein Synthesis Detection by Click Chemistry in Colorectal Cancer Patient-Derived Organoids Grown In Vitro

STAR Protocols, 2020
Summary: Here, we describe a protocol to detect and visualize protein synthesis by click-chemistry-based immunofluorescence in patient-derived organoids (PDOs) in vitro.
Clara Morral, Jelena Stanisavljevic, Eduard Batlle   +2 more
doaj   +1 more source

Structure-guided selection of puromycin N-acetyltransferase mutants with enhanced selection stringency for deriving mammalian cell lines expressing recombinant proteins

Scientific Reports, 2021
Puromycin and the Streptomyces alboniger-derived puromycin N-acetyltransferase (PAC) enzyme form a commonly used system for selecting stably transfected cultured cells.
Alessandro T. Caputo, Oliver M. Eder, Hana Bereznakova, Heleen Pothuis, Albert Ardevol, Janet Newman, Stewart Nuttall, Thomas S. Peat, Timothy E. Adams   +8 more
doaj   +1 more source

Retrograde trafficking of Argonaute 2 acts as a rate-limiting step for de novo miRNP formation on endoplasmic reticulum–attached polysomes in mammalian cells [PDF]

, 2020
microRNAs are short regulatory RNAs in metazoan cells. Regulation of miRNA activity and abundance is evident in human cells where availability of target messages can influence miRNA biogenesis by augmenting the Dicer1-dependent processing of precursors ...
Barman, Bahnisikha, Bhattacharyya, Suvendra N., Bose, Mainak, Chakrabarty, Yogaditya, Chatterjee, Susanta   +4 more
core