Results 41 to 50 of about 159,015 (286)
Scientific Reports, 2022 Podocytes are highly specialized cells playing a key role in the filtration function of the kidney. A damaged podocyte ultrastructure is associated with a reorganization of the actin cytoskeleton and accompanied with a loss of adhesion to the glomerular ...
Stefanie Jeruschke +3 more
doaj +1 more source
STAR Protocols, 2023 Summary: Translation is a fundamental process of cellular behavior. Here, we present a protocol for measuring translation in Drosophila epithelial tissues using O-propargyl-puromycin (OPP), a puromycin derivative.
Marianthi Kiparaki, Nicholas E. Baker
doaj +1 more source
Bifunctional Protein Conferring Enhanced Green Fluorescence and Puromycin Resistance
BioTechniques, 2001 A new genetic marker was created in which sequences from enhanced green fluorescent protein were fused to those of puromycin N-acetyl transferase. The resulting fusion protein (EGFP-puro) conferred both green fluorescence and resistance to puromycin when
J. Abbate +4 more
doaj +1 more source
Protocol for assessing translational regulation in mammalian cell lines by OP-Puro labeling
STAR Protocols, 2022 Summary: Translational regulation is a fundamental step in gene expression with critical roles in biological processes within a cell. Here, we describe a protocol to assess translation activity in mammalian cells by incorporation of O-propargyl-puromycin
Jack Chun-Chieh Hsu +3 more
doaj +1 more source
Sensitization of renal carcinoma cells to TRAIL-induced apoptosis by rocaglamide and analogs [PDF]
, 2018 Rocaglamide has been reported to sensitize several cell types to TRAIL-induced apoptosis. In recent years, advances in synthetic techniques have led to generation of novel rocaglamide analogs.
Brown, Lauren E. +5 more
core +2 more sources
Screening of AAK1 interaction proteins and its role in regulating global translation level in cells
Shanghai Jiaotong Daxue xuebao. Yixue ban, 2023 Objective·To investigate noval interacting partners for adaptor-associated protein kinase 1 (AAK1) and AAK1-mediated biological functions besides clathrin-mediated endocytosis.Methods·The labeled AAK1 vector and the blank control vector were transfected ...
JIANG Guixian, HU Ronggui, WU Hao
doaj +1 more source
Function‐driven design of a surrogate interleukin‐2 receptor ligand
FEBS Letters, EarlyView.Interleukin (IL)‐2 signaling can be achieved and precisely fine‐tuned through the affinity, distance, and orientation of the heterodimeric receptors with their ligands. We designed a biased IL‐2 surrogate ligand that selectively promotes effector T and natural killer cell activation and differentiation. Interleukin (IL) receptors play a pivotal role in
Ziwei Tang +9 more
wiley +1 more source
Investigation of protein synthesis in Drosophila larvae using puromycin labelling
Biology Open, 2017 Translational control of gene expression is an important regulator of growth, homeostasis and aging in Drosophila. The ability to measure changes in protein synthesis in response to genetic and environmental cues is therefore important in studying these ...
Lisa P. Deliu +2 more
doaj +1 more source
Lysosomal Delivery of Bioactive Proteins to Living Human Cells via Engineered Exosomes [PDF]
, 2018 Exosomes are naturally secreted nanovesicles derived from mammalian cells that are used for intercellular communication in vivo. As a result, they can potentially be used for intracellular delivery of therapeutics for disease treatment. We have developed
Levy, Daniel
core +1 more source
Analysis of the Role of Aminoacyl tRNA Synthetase Genes in Global Protein Synthesis and mRNA Specific Regulation of Translation in Cancer Cells [PDF]
, 2020 Analysis of the Role of Aminoacyl tRNA Synthetase Genes in Global Protein Synthesis and mRNA Specific Regulation of Translation in Cancer Cells Elyse Nguyen, Depts. of Biology and Chemistry, Dipak Poria, & Esta Sterneck, with Dr. Sarah Williams, Dept. of
Nguyen, Elyse +2 more
core +1 more source