Results 61 to 70 of about 159,015 (286)

Puromycin inhibition of cholesterol absorption in the rat

Journal of Lipid Research, 1977
The effect of puromycin on the intestinal absorption of cholesterol has been studied in rats with indwelling catheters in the left thoracic lymphatic duct.
G V Vahouny, M Ito, E M Blendermann, L L Gallo, C R Treadwell   +4 more
doaj   +1 more source

RUNX-mediated growth arrest and senescence are attenuated by diverse mechanisms in cells expressing RUNX1 fusion oncoproteins [PDF]

, 2018
RUNX gene over-expression inhibits growth of primary cells but transforms cells with tumor suppressor defects, consistent with reported associations with tumor progression.
Anderson, Gail, Bell, Margaret, Borland, Gillian, Cameron, Ewan, Gilroy, Kathryn, Hassanudin, Siti Ayuni, Hay, Jodie, Kilbey, Anna, Mackay, Nancy, McDonald, Alma, Neil, James C.   +10 more
core   +1 more source

Combining antibody conjugates with cytotoxic and immune‐stimulating payloads maximizes anti‐cancer activity

Molecular Oncology, EarlyView.
Methods to improve antibody–drug conjugate (ADC) treatment durability in cancer therapy are needed. We utilized ADCs and immune‐stimulating antibody conjugates (ISACs), which are made from two non‐competitive antibodies, to enhance the entry of toxic payloads into cancer cells and deliver immunostimulatory agents into immune cells.
Tiexin Wang, Dong Jun Koo, Peter M. Tessier, Greg M. Thurber   +3 more
wiley   +1 more source

Inactivation of an integrated antibiotic resistance gene in mammalian cells to re-enable antibiotic selection

BioTechniques, 2014
Removing an antibiotic resistance gene allows the same antibiotic to be re-used in the next round of genetic manipulation. Here we applied the CRISPR/Cas system to disrupt the puromycin resistance gene in an engineered mouse embryonic stem cell line and ...
Peiling Ni, Qian Zhang, Haixia Chen, Lingyi Chen   +3 more
doaj   +1 more source

Genetic attenuation of ALDH1A1 increases metastatic potential and aggressiveness in colorectal cancer

Molecular Oncology, EarlyView.
Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova, Zuzana Kozovska, Ondrej Pos, Kristina Pavlov, Sachin Gulati, Peter Makovicky, Kristina Jakic, Monika Burikova, Eva Sedlackova, Barbora Svitkova, Silvia Tyciakova, Vojtech Bystry, Nicolas Blavet, Boris Tichy, Matej Hrnciar, Jaroslav Budis, Miroslav Tomas, Peter Dubovan, Georgina Kolnikova, Veronika Repaska, Nikoleta Mojzesova, Eva Zomborska, Daniel Pindak, Michal Mego, Tomas Szemes, Miroslava Matuskova   +25 more
wiley   +1 more source

Visualisation of in vivo protein synthesis during mycobacterial infection through [68Ga]Ga-DOTA-puromycin µPET/MRI

Scientific Reports
Radiolabelled puromycin analogues will allow the quantification of protein synthesis through nuclear medicine-based imaging. A particularly useful application could be the non-invasive longitudinal visualisation of mycobacterial activity through direct ...
Sebastian Eigner, Janke Kleynhans, Dennis R. Beckford Vera, Mike M. Sathekge, Katerina Eigner Henke, Thomas Ebenhan   +5 more
doaj   +1 more source

Attachment of Proteolytic Enzyme Inhibitors to Vascular Prosthesis—An Analysis of Binding and Antimicrobial Properties

Molecules
Prosthetic infections are associated with high morbidity, mortality, and relapse rates, making them still a serious problem for implantology. Staphylococcus aureus is one of the most common bacterial pathogens causing prosthetic infections.
Aleksandra Mordzińska-Rak, Katarzyna Szałapata, Jerzy Wydrych, Mariusz Gagoś, Anna Jarosz-Wilkołazka   +4 more
doaj   +1 more source

Recombinant Cell-Permeable Puromycin <i>N</i>-Acetyltransferase Confers Puromycin Resistance by Direct Protein Transduction. [PDF]

J Microbiol Biotechnol
Choi J, Cho KH, Im J, Seo Y, Sharma A, Kartic, Devi S, Stalin N, Park SJ, Park TS.   +9 more
europepmc   +2 more sources

A novel puromycin decorporation method to quantify skeletal muscle protein breakdown: a proof-of-concept study [PDF]

, 2017
The precise roles that the major proteolytic pathways play in the regulation of skeletal muscle mass remain incompletely understood, in part due to technical limitations associated with current techniques used to quantify muscle protein breakdown (MPB ...
Atherton, Attaix, Bartoli, Bodine, Bonaldo, Costelli, Dai, Daniel J. Wilkinson, Desgeorges, Du, Du, Dunlop, Egan, Elia, Fielding, Fulks, Ghislat, Goodman, Goodman, Greenhaff, Hannah Crossland, Kenneth Smith, Li, Li, Long, Menconi, Millward, Philip J. Atherton, Sandri, Schmidt, Young   +30 more
core   +1 more source

Imaging of protein synthesis with puromycin [PDF]

Proceedings of the National Academy of Sciences, 2012
With great interest, we read the work by Liu et al. (1), which described the development of an alkyne analog of puromycin for labeling and imaging of newly synthesized peptides. Available techniques for this type of measurement are very limited, and the work by Liu et al.
Craig A, Goodman, Philippe, Pierre, Troy A, Hornberger   +2 more
openaire   +2 more sources