Results 71 to 80 of about 993 (122)

Expanding Genetic Code for Protein Lysine and Phenylalanine Modifications [PDF]

, 2015
The naturally occurring pyrrolysine (Pyl) incorporation machinery was discovered in methanogenic archaea and some bacteria. In these organisms, Pyl is cotranslationally inserted into proteins and coded by an in-frame UAG codon.
Wang, Yane-Shih 1977-
core   +1 more source

Rapid Identification of Functional Pyrrolysyl-tRNA Synthetases via Fluorescence-Activated Cell Sorting

, 2018
The orthogonal pyrrolysyl-tRNA synthetase/tRNACUA pair and their variants have provided powerful tools for expanding the genetic code to allow for engineering of proteins with augmented structure and function not present in Nature.
Andrew E. Lin, Qing Lin
core   +1 more source

Fully Productive Cell-Free Genetic Code Expansion by Structure-Based Engineering of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase

, 2020
Pyrrolysyl-tRNA synthetase (PylRS)/tRNAPyl pairs from Methanosarcina mazei and Methanosarcina barkeri are widely used for site-specific incorporations of non-canonical amino acids into proteins (genetic code expansion).
Tatsuo Yanagisawa (1428739), Kensaku Sakamoto (1428748), Shigeyuki Yokoyama (19189), Eiko Seki (826135), Mitsuo Kuratani (4262695)   +4 more
core   +1 more source

Efficient Site-specific Incorporation of ᴅ-Cysteinyl-Nᵋ-lysine into Recombinant Proteins in Escherichia coli

, 2021
Pyrrolysine is the 22nd genetically encoded amino acid found in methanogenic archaea and bacteria. The aminoacyl-tRNA synthetase(aa-RS)/tRNA pair specific for pyrrolysine i.e.

core  

An Evolved Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase/tRNA Pair Is Highly Active and Orthogonal in Mammalian Cells

, 2018
We recently characterized a new class of pyrrolysyl-tRNA synthetase (PylRS)/PyltRNA pairs from Methanomassiliicocales that are active and orthogonal in Escherichia coli.
Julian C. W. Willis (5796869), Václav Beránek (1356387), Julian C. W. Willis, Jason W. Chin (138646), Václav Beránek, Jason W. Chin   +5 more
core   +1 more source

Cell type-specific proteome labeling by genetic code expansion [PDF]

, 2016
의과대학/석사Analyzing gene expression of specific cells in a living organism is crucial in understanding complex biological systems. It has not been possible, however, to isolate the proteome of specific cells in vertebrates. Recently, advance in genetic code
김은진
core  

Exprimierte Zyklopeptid-Bibliotheken als selektive Inhibitoren für RNA-Protein Interaktionen / Strukturelle Grundlagen für den ortsspezifischen Einbau unnatürlicher Aminosäuren in Proteine und Peptide

, 2014
In the first part of the thesis, an in vivo screening assay was developed, which allows the identification of expressed cyclic peptides that specifically bind to bacterial riboswitches and inhibit downstream gene expression.
Flügel, Veronika M.
core  

Genetic Incorporation of Diverse Noncanonical Amino Acids for Histidine Substitution. [PDF]

J Am Chem Soc
Natter Perdiguero A, Fischer S, Lischke AR, Manser BP, Deliz Liang A.   +4 more
europepmc   +2 more sources

Photocaged FLP recombinase for precise spatio-temporal control of gene expression. [PDF]

Nucleic Acids Res
Rodriguez JJV, Baxter K, Ma Y, Greiss S.
europepmc   +2 more sources

Genomically integrated orthogonal translation system in Escherichia coli enables production of functional modified [NiFe]-hydrogenases. [PDF]

Microb Cell Fact
Fan Q, Stevanie S, Frielingsdorf S, Neubauer P, Lenz O, Gimpel M.   +5 more
europepmc   +1 more source