ChemistryOpen, 2019 Guanidinocalix[5]arene and fluorescein reporter pair has been chosen to set up a supramolecular tandem assay principle based on the differential recognition of pyridoxal‐5′‐phosphate (the substrate of alkaline phosphatase, ALP), pyridoxal (the product of
Yu‐Xin Yue +5 more
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Pyridoxal 5'-phosphate is a slow tight binding inhibitor of E. coli pyridoxal kinase. [PDF]
PLoS ONE, 2012 Pyridoxal 5'-phosphate (PLP) is a cofactor for dozens of B(6) requiring enzymes. PLP reacts with apo-B(6) enzymes by forming an aldimine linkage with the ε-amino group of an active site lysine residue, thus yielding the catalytically active holo-B(6 ...
Mohini S Ghatge +10 more
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Direct visualization of critical hydrogen atoms in a pyridoxal 5′-phosphate enzyme
Nature Communications, 2017 Pyridoxal 5’-phosphate (PLP) is a ubiquitous co factor for diverse enzymes, among them aspartate aminotransferase. Here the authors use neutron crystallography, which allows the visualization of the positions of hydrogen atoms, and computation to ...
Steven Dajnowicz +8 more
doaj +2 more sources
Mining the cellular inventory of pyridoxal phosphate-dependent enzymes with functionalized cofactor mimics. [PDF]
Nat Chem, 2018 Pyridoxal phosphate (PLP) is an enzyme cofactor required for the chemical transformation of biological amines in many central cellular processes. PLP-dependent enzymes (PLP-DEs) are ubiquitous and evolutionarily diverse, making their classification based
Hoegl A +7 more
europepmc +2 more sources
Arginase 2 Suppresses Renal Carcinoma Progression via Biosynthetic Cofactor Pyridoxal Phosphate Depletion and Increased Polyamine Toxicity. [PDF]
Cell Metab, 2018 Ochocki JD +18 more
europepmc +2 more sources
Mutations in PROSC Disrupt Cellular Pyridoxal Phosphate Homeostasis and Cause Vitamin-B6-Dependent Epilepsy. [PDF]
Am J Hum Genet, 2016 Darin N +19 more
europepmc +2 more sources
Biosynthesis of Ephedrine Initiated by Pyridoxal Phosphate-Dependent Formation of Cathinone. [PDF]
ChembiochemAn alternative pathway to ephedra alkaloids via (S)–cathinone is investigated in plants. This pathway circumvents the formation of 1‐phenylpropane‐1,2‐dione as an intermediate, which has been part of the predominantly postulated biosynthetic pathway for the last decades. This alternative pathway involves an enzymatic pyridoxal phosphate (PLP)‐dependent
Witte K +4 more
europepmc +2 more sources
Simultaneous Determination of Underivatized Vitamin B1 and B6 in Whole Blood by Reversed Phase Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry. [PDF]
PLoS ONE, 2015 Vitamin B1 (thiamine-diphosphate) and B6 (pyridoxal-5'phosphate) are micronutrients. Analysis of these micronutrients is important to diagnose potential deficiency which often occurs in elderly people due to malnutrition, in severe alcoholism and in ...
Johan Puts +3 more
doaj +1 more source
Emergence of oxygen‐ and pyridoxal phosphate‐dependent reactions
The FEBS Journal, 2020 Pyridoxal 5′‐phosphate (PLP) is an organic cofactor employed by ~ 4% of enzymes. The structure of the PLP cofactor allows for the stabilization of carbanions through resonance.
E. Hoffarth +2 more
semanticscholar +1 more source
NRF2 signalling in cytoprotection and metabolism
British Journal of Pharmacology, EarlyView., 2023 The KEAP1‐NRF2 system plays a central role in cytoprotection in defence mechanisms against oxidative stress. The KEAP1‐NRF2 system has been regarded as a sulfur‐utilizing cytoprotective mechanism, because KEAP1 serves as a biosensor for electrophiles by using its reactive thiols and NRF2 is a transcriptional factor regulating genes involved in sulfur ...
Shohei Murakami +4 more
wiley +1 more source