Results 201 to 210 of about 16,097 (238)
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An Enzymatic Assay for Pyrimidine Dimers in DNA
1975An assay for pyrimidine dimers in DNA employing the T4 endonuclease V is described. The assay can detect approximately 50 dimers per Escherichia coli genome, or the effect of 10 ergs/mm2 ultraviolet irradiation.
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Photosensitized splitting of pyrimidine dimers by indole derivatives
Biochemical and Biophysical Research Communications, 1971Abstract Excitation of tryptophan and 5-hydroxytryptophan at wavelengths longer than 290 and 310 nm, respectively, in the presence of pyrimidine dimers leads to a sensitized splitting of these dimers. The reaction is more efficient in frozen than in fluid aqueous solutions. The fluorescence of tryptophan and 5-hydroxytryptophan is quenched by thymine
C, Helene, M, Charlier
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PHOTOREPAIR OF NONADJACENT PYRIMIDINE DIMERS BY DNA PHOTOLYASE
Photochemistry and Photobiology, 1995Abstract— Photolyases reverse the harmful effects of UV light on cells by converting pyrimidine dimers (Pyr[]Pyr) into two pyrimidine monomers by utilizing near‐UV and visible light. Previous work has shown that photolyase repairs T[c,s]T and T[t,s]T in DNA as well as U[]U in RNA, all of which are formed by joining the two adjacent pyrimidines in a ...
S T, Kim, A, Sancar
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A rapid and sensitive assay for pyrimidine dimers in DNA
Analytical Biochemistry, 1973Abstract We have developed a rapid, sensitive assay for pyrimidine dimers. The assay has greatly facilitated the purification and characterization of the photoreactivating enzyme. The procedure depends on (1) the resistance of the nucleotide phosphate bond in dimer-containing regions of DNA to attack by DNase I, venom phosphodiesterase and alkaline ...
B M, Sutherland, M J, Chamberlin
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Mass spectra of some dimeric photoproducts of pyrimidines
Tetrahedron, 1969Abstract The mass spectra of photo-cycloaddition products of thymines and uracils and a photocoupled product of 5-bromouracil have been measured. Characteristic fragmentation has been documented for dimers joined through a cyclobutane ring, adducts derived from oxetane and azetidine linkages and a coupled product linked through a 5,5′-bond.
C, Fenselau, S Y, Wang
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The formation of pyrimidine dimers in the DNA of fungi and bacteria
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973Abstract The dose-related yields of the three kinds of pyrimidine dimer induced in DNA by ultraviolet light of 254 nm have been measured in three species of bacteria (Escherichia coli, Micrococcus luteus and Bacillus subtilis) and two species of fungi (Saccharomyces cerevisiae and Ustilago maydis).
P, Unrau +3 more
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EXCISION REPAIR OF PYRIMIDINE DIMERS IN MARSUPIAL CELLS
Photochemistry and Photobiology, 1987Monodelphis domestica was further characterized as a model for photobiological studies by measuring the excision repair capabilities of this mammal's cells both in vivo and in vitro. Excision repair capability of the established marsupial cell line, Pt K2 (Potorous tridactylus), was also determined.
L A, Applegate, R D, Ley
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Excision of pyrimidine dimers from the DNA of Neurospora
Molecular and General Genetics MGG, 1986Germinated conidia of Neurospora have been monitored for their ability to excise pyrimidine dimers. Dimer concentration was measured in DNA extracted immediately after UV treatment, and it was compared to that of DNA from cells which had a post-UV incubation before extraction. Two methods were used to assay dimer level in DNA: measurement of the number
H, Macleod, D, Stadler
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PHOTOREPAIR OF PYRIMIDINE DIMERS IN HUMAN SKIN IN VIVO
Photochemistry and Photobiology, 1981The exposure of human skin in vivo to UV radiation emitted from a sunlamp induces the formation of pyrimidine dimers. The number of dimers, as detected by UV-endonuclease, decreases following exposure of the UV-irradiated skin to visible wavelengths of light.
S M, D'Ambrosio +3 more
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A SENSITIVE METHOD FOR MEASURING PYRIMIDINE DIMERS IN SITU
Photochemistry and Photobiology, 1977Abstract. –An immunocytological method for detecting pyrimidine dimers in situ has been developed. The technique is based on the fixation in cell nuclei of radiolabelled antibodies directed against ultraviolet irradiated DNA. The relative amount of bound antibodies was estimated by radioautography. Pyrimidine dimers induced by a dose as low as 2 J m‐2
Cornelis, Jan +3 more
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