Results 131 to 140 of about 4,162 (161)
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Gene, 1996
A cDNA encoding delta 1-pyrroline-5-carboxylate reductase (P5CR) was isolated from the pneumocandin (Pmo)-producing fungus, Zalerion arboricola (Za), by complementation of a P5CR-deficient mutant (pro3) of Saccharomyces cerevisiae (Sc). The cloned cDNA was placed under control of the Sc galactokinase (GAL1) promoter and restored P5CR activity to the ...
R, Kelly, E, Register
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A cDNA encoding delta 1-pyrroline-5-carboxylate reductase (P5CR) was isolated from the pneumocandin (Pmo)-producing fungus, Zalerion arboricola (Za), by complementation of a P5CR-deficient mutant (pro3) of Saccharomyces cerevisiae (Sc). The cloned cDNA was placed under control of the Sc galactokinase (GAL1) promoter and restored P5CR activity to the ...
R, Kelly, E, Register
openaire +2 more sources
Biochimica Et Biophysica Acta - Biomembranes, 1980
A homogeneous preparation of delta 1-pyrroline-5-carboxylate reductase (L-proline: NAD (P)+ 5-oxidoreductase, EC 1.5.1.2) was obtained from baker's yeast by an affinity chromatography, using 5' AMP-Sepharose 4B. After the 1st DEAE-Sephadex column chromatography, the enzyme absorbed on 5' AMP-Sepharose column was eluted with 1 mM ATP.
Takeo Matsuzawa
exaly +3 more sources
A homogeneous preparation of delta 1-pyrroline-5-carboxylate reductase (L-proline: NAD (P)+ 5-oxidoreductase, EC 1.5.1.2) was obtained from baker's yeast by an affinity chromatography, using 5' AMP-Sepharose 4B. After the 1st DEAE-Sephadex column chromatography, the enzyme absorbed on 5' AMP-Sepharose column was eluted with 1 mM ATP.
Takeo Matsuzawa
exaly +3 more sources
1982
The discovery of a genetic defect in ornithine oxoacid aminotransferase (L-ornithine: 2-oxoacid aminotransferase, EC 2.6.1.13) in gyrate atrophy of the choroid and retina1,2,3, prompted us to study the ornithine metabolism of the eye4. We soon found high 361 activities of ornithine oxoacid aminotransferase and △1-pyrroline-5-carboxylate reductase (L ...
T, Matsuzawa +4 more
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The discovery of a genetic defect in ornithine oxoacid aminotransferase (L-ornithine: 2-oxoacid aminotransferase, EC 2.6.1.13) in gyrate atrophy of the choroid and retina1,2,3, prompted us to study the ornithine metabolism of the eye4. We soon found high 361 activities of ornithine oxoacid aminotransferase and △1-pyrroline-5-carboxylate reductase (L ...
T, Matsuzawa +4 more
openaire +2 more sources
Journal of Inherited Metabolic Disease, 2014
AbstractWe have conducted biochemical studies with commercial available pyrroline‐5‐carboxylate (P5C) reductase (PYCR1) to investigate whether this enzyme plays a role in L‐lysine degradation. Our recent studies with antiquitin/ALDH7A1 deficient fibroblasts revealed an alternative genesis of L‐pipecolic acid, and we then hypothesized that PYCR1 was ...
Struys, E.A. +2 more
openaire +4 more sources
AbstractWe have conducted biochemical studies with commercial available pyrroline‐5‐carboxylate (P5C) reductase (PYCR1) to investigate whether this enzyme plays a role in L‐lysine degradation. Our recent studies with antiquitin/ALDH7A1 deficient fibroblasts revealed an alternative genesis of L‐pipecolic acid, and we then hypothesized that PYCR1 was ...
Struys, E.A. +2 more
openaire +4 more sources
Biochimica et biophysica acta, 1981
delta 1-Pyrroline-5-carboxylate reductase (L-proline:NAD(P)+ 5-oxidoreductase, EC 1.5.1.2) from Baker's yeast has been purified and characterized. Purification to an apparently homogenous protein was effected by using 'reagent-grade' water containing dithiothreitol and by maintaining a constant pH 7.5, because of the instability of the enzyme protein ...
T, Matsuzawa, I, Ishiguro
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delta 1-Pyrroline-5-carboxylate reductase (L-proline:NAD(P)+ 5-oxidoreductase, EC 1.5.1.2) from Baker's yeast has been purified and characterized. Purification to an apparently homogenous protein was effected by using 'reagent-grade' water containing dithiothreitol and by maintaining a constant pH 7.5, because of the instability of the enzyme protein ...
T, Matsuzawa, I, Ishiguro
openaire +1 more source
Molecular and General Genetics MGG, 1990
We have isolated several cDNA clones encoding delta 1-pyrroline-5-carboxylate reductase (P5CR, L-proline: NAD(P)+ 5-oxidoreductase, EC 1.5.1.2) which catalyzes the terminal step in proline biosynthesis, by direct complementation of a proC mutation in Escherichia coli with an expression library of soybean root nodule cDNA. The library was constructed in
A J, Delauney, D P, Verma
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We have isolated several cDNA clones encoding delta 1-pyrroline-5-carboxylate reductase (P5CR, L-proline: NAD(P)+ 5-oxidoreductase, EC 1.5.1.2) which catalyzes the terminal step in proline biosynthesis, by direct complementation of a proC mutation in Escherichia coli with an expression library of soybean root nodule cDNA. The library was constructed in
A J, Delauney, D P, Verma
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International Journal of Biological Macromolecules
Osteosarcoma (OS) is a highly aggressive bone malignancy with limited effective biomarkers and therapeutic strategies. An integrative analysis was conducted combining transcriptomic profiling, mitochondrial-related gene (MRG) screening, and machine learning approaches to uncover novel diagnostic and prognostic markers.
Pan, Jang +8 more
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Osteosarcoma (OS) is a highly aggressive bone malignancy with limited effective biomarkers and therapeutic strategies. An integrative analysis was conducted combining transcriptomic profiling, mitochondrial-related gene (MRG) screening, and machine learning approaches to uncover novel diagnostic and prognostic markers.
Pan, Jang +8 more
openaire +2 more sources
Disease variants of human Δ1-pyrroline-5-carboxylate reductase 2 (PYCR2)
Archives of Biochemistry and Biophysics, 2021Javier Seravalli +2 more
exaly