Results 91 to 100 of about 1,475 (148)

Directed evolution of aminoacyl-tRNA synthetases through in vivo hypermutation. [PDF]

Genetic code expansion (GCE) is a critical approach to the site-specific incorporation of non-canonical amino acids (ncAAs) into proteins. Central to GCE is the development of orthogonal aminoacyl-tRNA synthetase (aaRS)/tRNA pairs wherein engineered ...
Furuhata, Yuichi, Liu, Chang, Rix, Gordon, Van Deventer, James   +3 more
core   +1 more source

Design einer polyspezifischen Pyrrolysyl-tRNA Synthetase für den Einbau biotinylierter unnatürlicher Aminosäuren [PDF]

, 2018
Die Pyrrolysyl-tRNA-Synthetase wird verwendet um das Amber-Codon für den Einbau von unnatürliche Aminosäuren neu zu definieren. Der Einbau neuer unnatürliche Aminosäuren erfordert oft einen umständlichen Engineering-Prozess der Pyrrolysyl-tRNA-Synthetase.
openaire  

Linker and N-Terminal Domain Engineering of Pyrrolysyl-tRNA Synthetase for Substrate Range Shifting and Activity Enhancement. [PDF]

Front Bioeng Biotechnol, 2020
Jiang HK, Lee MN, Tsou JC, Chang KW, Tseng HW, Chen KP, Li YK, Wang YS.   +7 more
europepmc   +1 more source

Engineered triply orthogonal pyrrolysyl-tRNA synthetase/tRNA pairs enable the genetic encoding of three distinct non-canonical amino acids. [PDF]

Nat Chem, 2020
Dunkelmann DL, Willis JCW, Beattie AT, Chin JW.   +3 more
europepmc   +1 more source

Machine learning-guided evolution of pyrrolysyl-tRNA synthetase for improved incorporation efficiency of diverse noncanonical amino acids. [PDF]

Nat Commun
Zhang Q, Jiang L, Niu Y, Li Y, Chen W, Cheng J, Ding H, Chen B, Liu K, Cao J, Wang J, Ye S, Yang L, Wu J, Xu G, Lin J, Yu H.   +16 more
europepmc   +1 more source

Directed Evolution of Candidatus Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase for the Genetic Incorporation of Two Different Noncanonical Amino Acids in One Protein. [PDF]

ACS Bio Med Chem Au
Cho CD, Leeuwon WM, Liu WR.
europepmc   +1 more source

Strategies for synthetic activation of translation elongation factor P [PDF]

, 2020
Volkwein, Wolfram
core   +2 more sources

Pyrrolysyl-tRNA Synthetase with a Unique Architecture Enhances the Availability of Lysine Derivatives in Synthetic Genetic Codes. [PDF]

Molecules, 2018
Yamaguchi A, Iraha F, Ohtake K, Sakamoto K.   +3 more
europepmc   +1 more source

An Evolved Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase/tRNA Pair Is Highly Active and Orthogonal in Mammalian Cells. [PDF]

Biochemistry, 2019
Beránek V, Willis JCW, Chin JW.
europepmc   +1 more source

An aminoacyl-tRNA synthetase that specifically activates pyrrolysine [PDF]

Burke, Paul, Hao, Srinivasan, Polycarpo, Ibba, Ambrogelly, Varshney, Pomerantz, Ibba, Brevet, Watanabe, Agris, Kowalak, Kast   +14 more
core   +1 more source