Results 71 to 80 of about 71,291 (119)

Ribosomal Incorporation of Thioxanthone as a Noncanonical Amino Acid Facilitates the Engineering of Photoenzymes

ChemCatChem, Volume 17, Issue 20, October 20, 2025.
Thioxanthone is an efficient visible‐light photosensitizer and facilitates reactions that are not known from natural enzymes. It is now possible to incorporate thioxanthone as a noncanonical amino acid (thioX) into proteins by an engineered amino acyl tRNA synthetase.
Marco Seifert, Martin Termathe, Luca Nardo, Matthias Höhne   +3 more
wiley   +1 more source

Genetically encoded Nδ-vinyl histidine for the evolution of enzyme catalytic center

Nature Communications
Genetic code expansion has emerged as a powerful tool for precisely introducing unnatural chemical structures into proteins to improve their catalytic functions.
Haoran Huang, Tao Yan, Chang Liu, Yuxiang Lu, Zhigang Wu, Xingchu Wang, Jie Wang   +6 more
doaj   +1 more source

Aligning fermentation conditions with non-canonical amino acid addition strategy is essential for Nε-((2-azidoethoxy)carbonyl)-L-lysine uptake and incorporation into the target protein

Scientific Reports
Protein engineering with non-canonical amino acids (ncAAs) holds great promises for diverse applications, however, there are still limitations in the implementation of this technology at manufacturing scale.
Hana Hanaee-Ahvaz, Marina Alexandra Baumann, Christopher Tauer, Bernd Albrecht, Birgit Wiltschi, Monika Cserjan-Puschmann, Gerald Striedner   +6 more
doaj   +1 more source

Quintuply orthogonal pyrrolysyl-tRNA synthetase/tRNA^Pyl pairs. [PDF]

Nat Chem, 2023
Beattie AT, Dunkelmann DL, Chin JW.
europepmc   +1 more source

Update of the Pyrrolysyl-tRNA Synthetase/tRNA^Pyl Pair and Derivatives for Genetic Code Expansion. [PDF]

J Bacteriol, 2023
Gong X, Zhang H, Shen Y, Fu X.
europepmc   +1 more source

Exploration of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase Activity in Yeast. [PDF]

ACS Synth Biol, 2022
Stieglitz JT, Lahiri P, Stout MI, Van Deventer JA.   +3 more
europepmc   +1 more source

A Designed, Highly Efficient Pyrrolysyl-tRNA Synthetase Mutant Binds o-Chlorophenylalanine Using Two Halogen Bonds. [PDF]

J Mol Biol, 2022
Vatansever EC, Yang KS, Geng ZZ, Qiao Y, Li P, Xu S, Liu WR.   +6 more
europepmc   +1 more source

Engineering Pyrrolysyl-tRNA Synthetase for the Incorporation of Non-Canonical Amino Acids with Smaller Side Chains. [PDF]

Int J Mol Sci, 2021
Koch NG, Goettig P, Rappsilber J, Budisa N.   +3 more
europepmc   +1 more source

The tRNA discriminator base defines the mutual orthogonality of two distinct pyrrolysyl-tRNA synthetase/tRNAPyl pairs in the same organism. [PDF]

Nucleic Acids Res, 2022
Zhang H, Gong X, Zhao Q, Mukai T, Vargas-Rodriguez O, Zhang H, Zhang Y, Wassel P, Amikura K, Maupin-Furlow J, Ren Y, Xu X, Wolf YI, Makarova KS, Koonin EV, Shen Y, Söll D, Fu X.   +17 more
europepmc   +1 more source

Crystal Structure of Pyrrolysyl-tRNA Synthetase from a Methanogenic Archaeon ISO4-G1 and Its Structure-Based Engineering for Highly-Productive Cell-Free Genetic Code Expansion with Non-Canonical Amino Acids. [PDF]

Int J Mol Sci, 2023
Yanagisawa T, Seki E, Tanabe H, Fujii Y, Sakamoto K, Yokoyama S.   +5 more
europepmc   +1 more source