Results 61 to 70 of about 71,291 (119)

Genetic Encoding of a Trifunctional Photo‐Cross‐Linker with a Cleavable Alkyl Ester Moiety

ChemBioChem, Volume 27, Issue 3, 12 February 2026.
A novel noncanonical amino acid, DiZAAsu, possessing photo‐cross‐linking, biotinylation, and alkaline‐cleavable functionalities, is genetically encoded by an engineered pyrrolyl‐tRNA synthetase mutant. The alkyl ester moiety expands the design space of cleavable photo‐cross‐linkable amino acids.
Masahiro Takayama, Tomoya Tsubota, Takao Yamaguchi, Kosuke Chiba, Takumi Yoshida, Yoshiyuki Hari, Yu‐Shi Tian, Daisuke Takaya, Asuka Mori, Tomohito Tsukamoto, Kenji Ishimoto, Yukio Ago, Yoshiaki Okada, Kensaku Sakamoto, Takefumi Doi, Kaori Fukuzawa, Satoshi Obika, Shinsaku Nakagawa, Nobumasa Hino   +18 more
wiley   +1 more source

Orthogonality and Codon Preference of the Pyrrolysyl-tRNA Synthetase-tRNAPyl pair in Escherichia coli for the Genetic Code Expansion [PDF]

, 2012
Systematic studies of basal nonsense suppression, orthogonality of tRNAPyl variants, and cross recognition between codons and tRNA anticodons are reported. E.
Odoi, Keturah
core   +3 more sources

Genetische Codierung von Pentafluorphosphato‐Phenylalanin ermöglicht die Herstellung von PF5‐Proteinen als Phosphoprotein‐Mimetika

Angewandte Chemie, Volume 138, Issue 1, 2 January 2026.
Erweiterung des genetischen Codes mit einem einzigartigen hyperfluorierten Phosphotyrosin‐Analogon. In dieser Arbeit haben wir die nicht‐natürliche Aminosäure Pentafluorphosphato‐Difluormethyl‐Phenylalanin, die sieben Fluoratome und eine permanente negative Ladung trägt, mittels mutierter, orthogonaler Aminoacyl‐tRNA‐Synthetasen erfolgreich in drei ...
Anna Magdalena Ambros, Haocheng Qianzhu, Markus Tiemann, Edan Habel, Katrin Denzinger, Hana Zupan, Matteo Accorsi, Bettina G. Keller, Gerhard Wolber, Thomas Huber, Jörg Rademann   +10 more
wiley   +1 more source

Polyspecific pyrrolysyl-tRNA synthetases from directed evolution

, 2014
Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA(Pyl) have emerged as ideal translation components for genetic code innovation. Variants of the enzyme facilitate the incorporation >100 noncanonical amino acids (ncAAs) into proteins.
Eiler, D, Wang, YS, Soll, D, Steitz, TA, Kiessling, LL, Nakamura, A, Kavran, JM, Wong, M, O'Donoghue, P, Guo, LT   +9 more
core   +1 more source

Genetic Encoding of Pentafluorophosphato‐Phenylalanine Provides PF5‐Proteins as Phosphoprotein Mimetics

Angewandte Chemie International Edition, Volume 65, Issue 1, 2 January 2026.
Genetic code expansion with a unique hyper‐fluorinated phosphotyrosine analog. In this work, we successfully incorporated the unnatural amino acid pentafluorophosphato‐difluoromethyl‐phenylalanine, carrying seven fluorine atoms and a permanent negative charge into three different proteins via the use of mutated orthogonal aminoacyl‐tRNA synthetases ...
Anna Magdalena Ambros, Haocheng Qianzhu, Markus Tiemann, Edan Habel, Katrin Denzinger, Hana Zupan, Matteo Accorsi, Bettina G. Keller, Gerhard Wolber, Thomas Huber, Jörg Rademann   +10 more
wiley   +1 more source

Design of a polyspecific pyrrolysyl-tRNA synthetase for the incorporation of biotinylated unnatural amino acids

, 2018
Die Pyrrolysyl-tRNA-Synthetase wird verwendet um das Amber-Codon für den Einbau von unnatürliche Aminosäuren neu zu definieren. Der Einbau neuer unnatürliche Aminosäuren erfordert oft einen umständlichen Engineering-Prozess der Pyrrolysyl-tRNA-Synthetase.
Hohl, Adrian
core  

Protein engineering strategies to optimise recombinant product synthesis and accumulation in Nicotiana benthamiana

Plant Biotechnology Journal, Volume 24, Issue 1, Page 31-43, January 2026.
Summary In plant molecular farming, recombinant products include traditional protein targets, such as antibodies, antigens, and enzymes, but also increasingly refers to high‐value peptides, and small molecules produced by heterologous enzymatic pathways.
Maxim D. Harding, Frank Sainsbury
wiley   +1 more source

Efficient Multisite Unnatural Amino Acid Incorporation in Mammalian Cells via Optimized Pyrrolysyl tRNA Synthetase/tRNA Expression and Engineered eRF1

, 2015
The efficient, site-specific introduction of unnatural amino acids into proteins in mammalian cells is an outstanding challenge in realizing the potential of genetic code expansion approaches.
Wolfgang H. Schmied (1341927), Simon J. Elsässer (1307598), Jason W. Chin (138646), Chayasith Uttamapinant (1307595)   +3 more
core   +1 more source

DataSheet1_Efficient Unnatural Protein Production by Pyrrolysyl-tRNA Synthetase With Genetically Fused Solubility Tags.pdf

, 2022
Introducing non-canonical amino acids (ncAAs) by engineered orthogonal pairs of aminoacyl-tRNA synthetases and tRNAs has proven to be a highly useful tool for the expansion of the genetic code.
Tobias Baumann (1753615), Nikolaj G. Koch (12101261), Nediljko Budisa (129978)   +2 more
core   +1 more source

Internal Ubiquitin Electrophiles for Covalent Trapping and Inhibition of Deubiquitinases

ChemBioChem, Volume 26, Issue 22, November 17, 2025.
GA: Genetically encoded internal electrophiles in ubiquitin (Ub) enable the profiling of multivalent interaction interfaces, addressing a key limitation of current Ub‐based activity‐based probes. In this study, these probes capture a Ub–deubiquitinase interaction with minimal structural perturbation and serve as selective diUb probes that covalently ...
Nipuni M. Pannala, Rishi S. Patel, Abhijith Saseendran Anit, Debapriya Bhattacharya, Kristos Negron Teron, Bryon Drown, Rudi Fasan, Chittaranjan Das   +7 more
wiley   +1 more source