Results 41 to 50 of about 71,291 (119)

Exploration of Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase Activity in Yeast

, 2022
Archaeal pyrrolysyl-tRNA synthetases (PylRSs) have been used to genetically encode over 200 distinct noncanonical amino acids (ncAAs) in proteins in Escherichia coli and mammalian cells.
Jessica T. Stieglitz (5711864), Priyanka Lahiri (1666618), James A. Van Deventer, Priyanka Lahiri, Jessica T. Stieglitz, Matthew I. Stout, Matthew I. Stout (12413584), James A. Van Deventer (2653711)   +7 more
core   +1 more source

Engineering and analysis of pyrrolysyl-tRNA synthetase from Methanomethylophilus alvus for genetic code expansion beyond L-α-amino acids

, 2023
The cellular translation apparatus effectively synthesizes sequence-defined polymers composedof amino acids: proteins. For the most part, ribosomal protein synthesis only accesses the chemical diversity provided by the 20 canonical amino acids, but there
Fricke, Riley Carson Bryden
core   +1 more source

A Rationally Designed Pyrrolysyl-tRNA Synthetase Mutant with a Broad Substrate Spectrum

, 2016
Together with tRNACUAPyl, a rationally designed pyrrolysyl-tRNA synthetase mutant N346A/C348A has been successfully used for the genetic incorporation of a variety of phenylalanine derivatives with large para substituents into superfolder green ...
Bo Wu (16141), Xinqiang Fang (1904908), Wenshe R. Liu (387065), Ashley L. Wallace (2113606), Yane-Shih Wang (1316121)   +4 more
core   +1 more source

Expanding Genetic Code to Generate Human Brain Organoids with Both Vasculature and Microglia‐Like Cells

Advanced Science, EarlyView.
Using genetic code expansion, we engineered vascularized human cerebral organoids (vhCOs) with microglia‐like cells and blood‐brain barrier features. vhCOs recapitulate neurovascular interactions, regional identities, and neuronal subtypes resembling the fetal brain.
Haishuang Lin, Yaqing Wang, Hu Du, Yuxin Qin, Haoyu Zhang, Peng Wang, Lin Wei, Jianhua Qin   +7 more
wiley   +1 more source

Biochemical and structural characterization of mycobacterial aspartyl-tRNA synthetase AspS, a promising TB drug target [PDF]

, 2014
The human pathogen Mycobacterium tuberculosis is the causative agent of pulmonary tuberculosis (TB), a disease with high worldwide mortality rates. Current treatment programs are under significant threat from multi-drug and extensively-drug resistant ...
VijayaShankar Nataraj, Reynolds Robert C., Nicholas J Loman, Lloyd, AJ, Besra, Gurdyal S., Klaus Fütterer, Ballell, L, Reynolds, Robert C., Abrahams, Katherine A, Loman, Nicholas J., Hobrath Judith V., Gurcha Sudagar S., Alderwick, LJ, Abrahams, Katherine A., Gurcha, SS, Cox, Jonathan A G, Abrahams Katherine A., Hobrath, JV, Loman, Nicholas J, Carlos Alemparte, Lluis Ballell, Bhatt, Apoorva; id_orcid, Cox Jonathan A. G., Loman, NJ, Besra, GS, Reynolds, Robert C, Alemparte Carlos, Alderwick, Luke J, Gurcha, Sudagar S, Besra, Gurdyal S; id_orcid, Abrahams, KA, Besra Gurdyal S., Hobrath, Judith V, Barros, D, Robert C Reynolds, Loman Nicholas J., Bhatt, A, Ballell, Lluis, Nataraj, VijayaShankar, Lloyd, Adrian J., Apoorva Bhatt, Bhatt Apoorva, Nataraj, V, Alemparte, Carlos, Lloyd, Adrian J, Fütterer, K, Hobrath, Judith V., Veeraraghavan Usha, Cox, Jonathan A. G., Cox, JA, Barros David, Veeraraghavan, Usha, Bhatt, Apoorva, Adrian J Lloyd, Jonathan A G Cox, Usha, V, Gurdyal S Besra, David Barros, Lloyd Adrian J., Reynolds, RC, Alderwick, Luke J., Ballell Lluis, Usha Veeraraghavan, Fütterer Klaus, Barros, David, Nataraj, Vijaya-Shankar, Luke J Alderwick, Gurcha, Sudagar S., Alderwick Luke J., Judith V Hobrath, Sudagar S Gurcha, Usha, Veeraraghavan, Nataraj VijayaShankar, Alemparte, C, Fütterer, Klaus, Katherine A Abrahams   +75 more
core   +1 more source

Efficient unnatural protein production by pyrrolysyl-tRNA synthetase with genetically fused solubility tags [PDF]

, 2022
Introducing non-canonical amino acids (ncAAs) by engineered orthogonal pairs of aminoacyl-tRNA synthetases and tRNAs has proven to be a highly useful tool for the expansion of the genetic code.
Baumann, Tobias, Koch, Nikolaj G., Budisa, Nediljko   +2 more
core   +1 more source

Simplified methodology for a modular and genetically expanded protein synthesis in cell-free systems

Synthetic and Systems Biotechnology, 2019
Genetic code expansion, which enables the site-specific incorporation of unnatural amino acids into proteins, has emerged as a new and powerful tool for protein engineering.
Yonatan Chemla, Eden Ozer, Michael Shaferman, Ben Zaad, Rambabu Dandela, Lital Alfonta   +5 more
doaj   +1 more source

Methanomethylophilus alvus Mx1201 Provides Basis for Mutual Orthogonal Pyrrolysyl tRNA/Aminoacyl-tRNA Synthetase Pairs in Mammalian Cells

, 2018
Genetic code expansion via stop codon suppression is a powerful technique for engineering proteins in mammalian cells with site-specifically encoded noncanonical amino acids (ncAAs).
Johannes Heimgärtner (5849411), Birthe Meineke (4432381), Simon J. Elsässer (1307598), Lorenzo Lafranchi (5849414)   +3 more
core   +1 more source

Methanomethylophilus alvus Mx1201 provides basis for mutual orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in mammalian cells.

, 2018
Genetic code expansion via stop codon suppression is a versatile tool for engineering proteins in mammalian cells with site-specifically encoded non-canonical amino acids (ncAAs).
Elsässer, S (via Mendeley Data)
core   +1 more source

Expanding the library and substrate diversity of the pyrrolysyl-tRNA synthetase to incorporate unnatural amino acids containing conjugated rings. [PDF]

Chembiochem, 2013
Unnatural amino acids (UAAs) containing conjugated ring systems are of interest for their optical properties. Until now, such bulky and planar UAAs could not be incorporated into proteins using the pyrrolysyl tRNA/synthetase shuttling system.
Lacey VK, Louie GV, Noel JP, Wang L.
europepmc   +2 more sources