Results 31 to 40 of about 71,291 (119)

Rational design of the genetic code expansion toolkit for in vivo encoding of D-amino acids

Frontiers in Genetics, 2023
Once thought to be non-naturally occurring, D-amino acids (DAAs) have in recent years been revealed to play a wide range of physiological roles across the tree of life, including in human systems.
Han-Kai Jiang, Han-Kai Jiang, Han-Kai Jiang, Jui-Hung Weng, Yi-Hui Wang, Yi-Hui Wang, Jo-Chu Tsou, Pei-Jung Chen, Pei-Jung Chen, An-Li Andrea Ko, Dieter Söll, Dieter Söll, Ming-Daw Tsai, Yane-Shih Wang, Yane-Shih Wang, Yane-Shih Wang   +15 more
doaj   +1 more source

Selenocysteine, pyrrolysine and the unique energy metabolism of methanogenic archaea [PDF]

, 2010
Methanogenic archaea are a group of strictly anaerobic microorganisms characterized by their strict dependence on the process of methanogenesis for energy conservation.
Rother, Michael, Krzycki, Joseph A., Joseph A. Krzycki, Michael Rother   +3 more
core   +1 more source

Site‐Specific Protein Bioconjugation Through Cellular Incorporation of Noncanonical Amino Acids

Angewandte Chemie, EarlyView.
Genetic code expansion (GCE) enables site‐specific installation of noncanonical amino acids containing bioorthogonal conjugation handles, allowing precise, homogeneous protein modification. This review examines the principles of orthogonal translation, surveys the chemistries available for chemoselective labeling, and highlights emerging multi‐site ...
Rahul Sarkar, Manas Joshi, Abhishek Chatterjee   +2 more
wiley   +2 more sources

Incorporation of Amino Acids with Long-Chain Terminal Olefins into Proteins

Molecules, 2016
The increasing need for site-specific protein decorations that mimic natural posttranslational modifications requires access to a variety of noncanonical amino acids with moieties enabling bioorthogonal conjugation chemistry.
Matthias P. Exner, Sebastian Köhling, Julie Rivollier, Sandrine Gosling, Puneet Srivastava, Zheni I. Palyancheva, Piet Herdewijn, Marie-Pierre Heck, Jörg Rademann, Nediljko Budisa   +9 more
doaj   +1 more source

Expanding the Scope of Orthogonal Translation with Pyrrolysyl-tRNA Synthetases Dedicated to Aromatic Amino Acids

Molecules, 2020
In protein engineering and synthetic biology, Methanosarcina mazei pyrrolysyl-tRNA synthetase (MmPylRS), with its cognate tRNAPyl, is one of the most popular tools for site-specific incorporation of non-canonical amino acids (ncAAs).
Hsueh-Wei Tseng, Tobias Baumann, Huan Sun, Yane-Shih Wang, Zoya Ignatova, Nediljko Budisa   +5 more
doaj   +1 more source

Isosteric Engineering of Enzymes: Overcoming Activity–Stability Trade‐Offs by Site‐Selective CH → N Substitutions

Angewandte Chemie, Volume 138, Issue 38, 14 September 2026.
Coupling biosynthetic noncanonical amino acid production with genetic code expansion enables site‐specific incorporation of azatryptophans into PET‐degrading enzymes (PETases). By isosteric single‐atom editing of a conserved tryptophan, AzaPETases break the activity–stability trade‐off, delivering higher catalytic efficiency at elevated temperature and
Elwy H. Abdelkader, Haocheng Qianzhu, Gottfried Otting, Thomas Huber   +3 more
wiley   +2 more sources

Expanding the Genetic Code of Lactococcus lactis and Escherichia coli to Incorporate Non-canonical Amino Acids for Production of Modified Lantibiotics

Frontiers in Microbiology, 2018
The incorporation of non-canonical amino acids (ncAAs) into ribosomally synthesized and post-translationally modified peptides, e.g., nisin from the Gram-positive bacterium Lactococcus lactis, bears great potential to expand the chemical space of various
Maike Bartholomae, Tobias Baumann, Jessica H. Nickling, David Peterhoff, Ralf Wagner, Nediljko Budisa, Oscar P. Kuipers   +6 more
doaj   +1 more source

Development of a high yielding expression platform for the introduction of non-natural amino acids in protein sequences

mAbs, 2020
The ability to genetically encode non-natural amino acids (nnAAs) into proteins offers an expanded tool set for protein engineering. nnAAs containing unique functional moieties have enabled the study of post-translational modifications, protein ...
Gargi Roy, Jason Reier, Andrew Garcia, Tom Martin, Megan Rice, Jihong Wang, Meagan Prophet, Ronald Christie, William Dall’Acqua, Sanjeev Ahuja, Michael A Bowen, Marcello Marelli   +11 more
doaj   +1 more source

Towards Reassignment of the Methionine Codon AUG to Two Different Noncanonical Amino Acids in Bacterial Translation

Croatica Chemica Acta, 2016
Genetic encoding of noncanonical amino acids (ncAAs) through sense codon reassignment is an efficient tool for expanding the chemical functionality of proteins. Incorporation of multiple ncAAs, however, is particularly challenging.
Alessandro De Simone, Carlos G. Acevedo-Rocha, Michael Georg Hoesl, Nediljko Budisa   +3 more
doaj   +1 more source

Expanding the Genetic Code of Yeast for Incorporation of Diverse Unnatural Amino Acids via a Pyrrolysyl-tRNA Synthetase/tRNA Pair

, 2015
We report the discovery of a simple system through which variant pyrrolysyl-tRNA synthetase/tRNACUAPyl pairs created in Escherichia coli can be used to expand the genetic code of Saccharomyces cerevisiae.
Rajendra Uprety (1273764), Jason W. Chin (138646), Susan M. Hancock (1273767), Alexander Deiters (624599)   +3 more
core   +1 more source