Pyrrolysyl-tRNA Synthetase, an Aminoacyl-tRNA Synthetase for Genetic Code Expansion [PDF]
Croatica Chemica Acta, 2016 Genetic code expansion (GCE) has become a central topic of synthetic biology. GCE relies on engineered aminoacyl‐tRNA synthetases (aaRSs) and a cognate tRNA species to allow codon reassignment by co-translational insertion of non-canonical amino acids ...
Ana Crnković +3 more
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Pyrrolysyl-tRNA synthetase variants reveal ancestral aminoacylation function [PDF]
FEBS Letters, 2013 Pyrrolysyl-tRNA synthetase (PylRS) is a class IIc aminoacyl-tRNA synthetase that is related to phenylalanyl-tRNA synthetase (PheRS). Genetic selection provided PylRS variants with a broad range of specificity for diverse non-canonical amino acids (ncAAs).
Dieter Söll +2 more
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Linker and N-Terminal Domain Engineering of Pyrrolysyl-tRNA Synthetase for Substrate Range Shifting and Activity Enhancement [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 The Methanosarcina mazei pyrrolysyl-tRNA synthetase (PylRS)⋅tRNAPyl pair can be used to incorporate non-canonical amino acids (ncAAs) into proteins at installed amber stop codons.
Han-Kai Jiang +11 more
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Expanding the genetic code: phage-driven evolution of pyrrolysyl-synthetase for site-specific incorporation of synthetic phenylalanine and tyrosine derivatives [PDF]
Frontiers in Molecular BiosciencesIntroductionPyrrolysyl-tRNA synthetase (PylRS) is a key enzyme for the site-specific incorporation of non-canonical amino acids (ncAAs) into proteins. However, its native form has a limited substrate scope.
Anastasia Dakhnevich +4 more
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An Expanded Toolbox for Versatile Chemical Editing of Adeno-Associated Virus. [PDF]
Angew Chem Int Ed EnglWe describe technology to introduce diverse non‐natural chemical functionalities site‐specifically into the capsid of adeno‐associated virus through genetic code expansion, and using them to engineer this leading vector for human gene therapy for enhanced tissue specificity and reduced immunogenicity Abstract Site‐specific incorporation of noncanonical
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Genomically integrated orthogonal translation system in Escherichia coli enables production of functional modified [NiFe]-hydrogenases [PDF]
Microbial Cell FactoriesThe functional diversification of O2-tolerant [NiFe]-hydrogenases using orthogonal translation systems (OTSs) offers a promising strategy for developing advanced biocatalysts and biohybrid energy platforms.
Qin Fan +5 more
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Genetic Incorporation of ϵ-N-Benzoyllysine by Engineering Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase. [PDF]
Chembiochem, 2021 Protein post-translational modifications regulate protein structure and function. Lysine benzoylation is a new type of histone mark with unique physiological relevance.
Cao L +4 more
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The Pyrrolysyl-tRNA Synthetase Activity can be Improved by a P188 Mutation that Stabilizes the Full-Length Enzyme [PDF]
Journal of Molecular Biology, 2022 Wenshe R Liu, Shiqing Xu, Xinyu Ma
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Directed Evolution of Candidatus Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase for the Genetic Incorporation of Two Different Noncanonical Amino Acids in One Protein [PDF]
ACS Bio & Med Chem AuChia-Chuan D. Cho +2 more
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tRNA shape is an identity element for an archaeal pyrrolysyl-tRNA synthetase from the human gut. [PDF]
Nucleic Acids ResProtein translation is orchestrated through tRNA aminoacylation and ribosomal elongation. Among the highly conserved structure of tRNAs, they have distinguishing features which promote interaction with their cognate aminoacyl tRNA synthetase (aaRS ...
Krahn N +11 more
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