Cell Reports: Methods, 2023 Summary: Stop codon suppression using dedicated tRNA/aminoacyl-tRNA synthetase (aaRS) pairs allows for genetically encoded, site-specific incorporation of non-canonical amino acids (ncAAs) as chemical handles for protein labeling and modification.
Birthe Meineke +7 more
doaj +1 more source
Pyrrolysyl-tRNA-Synthetase: Methanogenese und Gencode-Erweiterung [PDF]
, 2021 Pyrrolysyl-tRNA synthetase (PylRS) is an enzyme of some methanogenic Archaea for the natural incorporation of pyrrolysine into proteins. The discovery of PylRS as a natural tool for genetic code expansion paved the way for site-specific incorporation of ...
Koch, Nikolaj Georg, Budisa, Nediljko
core +1 more source
Nonsense and sense suppression abilities of original and derivative Methanosarcina mazei pyrrolysyl-tRNA synthetase-tRNA(Pyl) pairs in the Escherichia coli BL21(DE3) cell strain. [PDF]
PLoS ONE, 2013 Systematic studies of nonsense and sense suppression of the original and three derivative Methanosarcina mazei PylRS-tRNA(Pyl) pairs and cross recognition between nonsense codons and various tRNA(Pyl) anticodons in the Escherichia coli BL21(DE3) cell ...
Keturah A Odoi +3 more
doaj +1 more source
Evolution of the gene translation machinery and its applications to drug discovery [PDF]
, 2012 [eng] Gene translation is a central process in all cells, in which messenger RNA (mRNA) is decoded by the ribosome to produce a specific amino acid chain, that will later fold into an active protein.
Novoa Pardo, Eva Maria
core +6 more sources
Pyrrolysyl-tRNA synthetase: An ordinary enzyme but an outstanding genetic code expansion tool [PDF]
Biochimica Et Biophysica Acta - Proteins and Proteomics, 2014 Jeffery M Tharp, Wenshe R Liu
exaly +2 more sources
Two novel anti-aminoacyl tRNA synthetase antibodies: Autoantibodies against cysteinyl-tRNA synthetase and valyl-tRNA synthetase [PDF]
, 2022 Anti-aminoacyl-tRNA synthetase (anti-ARS) antibodies are useful for identifying a clinical subset of patients with inflammatory myopathies. Since the myositis of anti-ARS-positive patients is characterized by a unique set of non-myopathic manifestations,
Takeichi, Takuya +6 more
core +1 more source
PLoS ONE, 2020 Although whole-genome sequencing has provided novel insights into Neisseria meningitidis, many open reading frames have only been annotated as hypothetical proteins with unknown biological functions.
Hideyuki Takahashi +6 more
doaj +1 more source
Structural basis for the site-specific incorporation of lysine derivatives into proteins. [PDF]
PLoS ONE, 2014 Posttranslational modifications (PTMs) of proteins determine their structure-function relationships, interaction partners, as well as their fate in the cell and are crucial for many cellular key processes.
Veronika Flügel +2 more
doaj +1 more source
Frontiers in Molecular Biosciences, 2022 The investigation of protein structures, functions and interactions often requires modifications to adapt protein properties to the specific application.
Jeffrey L. Schloßhauer +7 more
doaj +1 more source
Mutant glycyl-tRNA synthetase (Gars) ameliorates SOD1G93A motor neuron degeneration phenotype but has little affect on Loa dynein heavy chain mutant mice [PDF]
, 2009 Background: In humans, mutations in the enzyme glycyl-tRNA synthetase (GARS) cause motor and sensory axon loss in the peripheral nervous system, and clinical phenotypes ranging from Charcot-Marie-Tooth neuropathy to a severe infantile form of spinal ...
Hazel P Williams +35 more
core +1 more source