Results 81 to 90 of about 71,291 (119)

Transferability of N-terminal mutations of pyrrolysyl-tRNA synthetase in one species to that in another species on unnatural amino acid incorporation efficiency. [PDF]

Amino Acids, 2021
Williams TL, Iskandar DJ, Nödling AR, Tan Y, Luk LYP, Tsai YH.   +5 more
europepmc   +1 more source

Engineering a promiscuous pyrrolysyl-tRNA synthetase by a high throughput FACS screen

, 2017
The Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNAPyl are used to facilitate the incorporation of non-canonical amino acids (ncAAs) into the genetic code of bacterial and eukaryotic cells by orthogonally reassigning the amber codon.
Michael Groll, Jörg Eppinger, Dominik Renn, Adrian Hohl, Ram Karan, Magnus Rueping, Anstassja Akal, Alaguraj Dharamarajnadar, Seema Ghoprade, Xuechao Liu   +9 more
core   +1 more source

Engineered triply orthogonal pyrrolysyl-tRNA synthetase/tRNA pairs enable the genetic encoding of three distinct non-canonical amino acids. [PDF]

Nat Chem, 2020
Dunkelmann DL, Willis JCW, Beattie AT, Chin JW.   +3 more
europepmc   +1 more source

Evolution of Pyrrolysyl-tRNA Synthetase: From Methanogenesis to Genetic Code Expansion. [PDF]

Chem Rev
Koch NG, Budisa N.
europepmc   +1 more source

Engineering a Polyspecific Pyrrolysyl-tRNA Synthetase by a High Throughput FACS Screen. [PDF]

Sci Rep, 2019
Hohl A, Karan R, Akal A, Renn D, Liu X, Ghorpade S, Groll M, Rueping M, Eppinger J.   +8 more
europepmc   +1 more source

Mutually orthogonal pyrrolysyl-tRNA synthetase/tRNA pairs. [PDF]

Nat Chem, 2018
Willis JCW, Chin JW.
europepmc   +1 more source

Crystal structures reveal an elusive functional domain of pyrrolysyl-tRNA synthetase. [PDF]

Nat Chem Biol, 2017
Suzuki T, Miller C, Guo LT, Ho JML, Bryson DI, Wang YS, Liu DR, Söll D.   +7 more
europepmc   +1 more source

An Evolved Methanomethylophilus alvus Pyrrolysyl-tRNA Synthetase/tRNA Pair Is Highly Active and Orthogonal in Mammalian Cells. [PDF]

Biochemistry, 2019
Beránek V, Willis JCW, Chin JW.
europepmc   +1 more source

Evolving the N-Terminal Domain of Pyrrolysyl-tRNA Synthetase for Improved Incorporation of Noncanonical Amino Acids. [PDF]

Chembiochem, 2018
Sharma V, Zeng Y, Wang WW, Qiao Y, Kurra Y, Liu WR.   +5 more
europepmc   +1 more source

An efficient pyrrolysyl-tRNA synthetase for economical production of MeHis-containing enzymes.

Faraday Discuss
Hutton AE, Foster J, Sanders JEJ, Taylor CJ, Hoffmann SA, Cai Y, Lovelock SL, Green AP.   +7 more
europepmc   +1 more source