Orthogonal pair-directed codon reassignment as a tool for evaluating the factors affecting translation in E. coli [PDF]
, 2018 2018 Spring.Includes bibliographical references.Proteins are polymers of amino acids that are essential for life, central to cellular function, and have applications in fields ranging from materials science to biomedicine. Proteins in nature are composed
Schwark, David
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An evolved pyrrolysyl-tRNA synthetase with polysubstrate specificity expands the toolbox for engineering enzymes with incorporation of noncanonical amino acids. [PDF]
Bioresour Bioprocess, 2023 Liu K +9 more
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Beyond In Vivo, Pharmaceutical Molecule Production in Cell-Free Systems and the Use of Noncanonical Amino Acids Therein [PDF]
Throughout history, we have looked to nature to discover and copy pharmaceutical solutions to prevent and heal diseases. Due to the advances in metabolic engineering and the production of pharmaceutical proteins in different host cells, we have moved ...
Abendroth, Ulrike +6 more
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Structural Basis for the Site-Specific Incorporation of Lysine Derivatives into Proteins [PDF]
, 2014 Posttranslational modifications (PTMs) of proteins determine their structure-function relationships, interaction partners, as well as their fate in the cell and are crucial for many cellular key processes.
Flügel, Veronika +2 more
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Implications of Lysine Acetylation on the Enzymes of the TCA Cycle [PDF]
, 2020 The tricarboxylic acid (TCA) cycle is a central route for the energy production via oxidative phosphorylation and is ubiquitous throughout nature. Apart from playing a major role in energy provision, it is also essential for a wide range of physiological
Venkat, Sumana
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The Pyrrolysyl-tRNA Synthetase Activity can be Improved by a P188 Mutation that Stabilizes the Full-Length Enzyme. [PDF]
J Mol Biol, 2022 Cho CC +4 more
europepmc +1 more source
Site directed mutagenesis of L-pyrrolysine in dimethylamine methyltransferase [PDF]
, 2009 Methanosarcina barkeri is an archaeon and is able to use methylamines as growth substrates for methanogenesis. The methylamine methyltransferases that are involved in initiating methanogenesis from the methylamines have an in-frame amber codon (UAG) that
Patel, Jigar
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The tRNA discriminator base defines the mutual orthogonality of two distinct pyrrolysyl-tRNA synthetase/tRNAPyl pairs in the same organism. [PDF]
Nucleic Acids Res, 2022 Zhang H +17 more
europepmc +1 more source
Crystal Structure of Pyrrolysyl-tRNA Synthetase from a Methanogenic Archaeon ISO4-G1 and Its Structure-Based Engineering for Highly-Productive Cell-Free Genetic Code Expansion with Non-Canonical Amino Acids. [PDF]
Int J Mol Sci, 2023 Yanagisawa T +5 more
europepmc +1 more source
Conformational transition of FGFR kinase activation revealed by site-specific unnatural amino acid reporter and single molecule FRET [PDF]
, 2017 Protein kinases share significant structural similarity; however, structural features alone are insufficient to explain their diverse functions. Thus, bridging the gap between static structure and function requires a more detailed understanding of ...
Alexandrov, Y +10 more
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