Label-free shotgun proteomics and metabolite analysis reveal a significant metabolic shift during citrus fruit development. [PDF]
, 2011 Label-free LC-MS/MS-based shot-gun proteomics was used to quantify the differential protein synthesis and metabolite profiling in order to assess metabolic changes during the development of citrus fruits.
Blumwald, Eduardo +8 more
core +3 more sources
Folding of the Protein Domain hbSBD [PDF]
Biophysical J. 89, 3353 (2005), 2006 The folding of the alpha-helice domain hbSBD of the mammalian mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex is studied by the circular dichroism technique in absence of urea. Thermal denaturation is used to evaluate various thermodynamic parameters defining the equilibrium unfolding, which is well described by the two-state ...
arxiv +1 more source
LC-MS proteomics analysis of the iInsulin/IGF-1-deficient Caenorhabditis elegans daf-2(e1370) mutant reveals extensive restructuring of intermediary metabolism [PDF]
, 2014 The insulin/IGF-1 receptor is a major known determinant of dauer formation, stress resistance, longevity, and metabolism in Caenorhabditis elegans. In the past, whole-genome transcript profiling was used extensively to study differential gene expression ...
Braeckman, Bart +7 more
core +2 more sources
Lin28A induces energetic switching to glycolytic metabolism in human embryonic kidney cells [PDF]
, 2016 Background: Loss of a cell’s capacity to generate sufficient energy for cellular functions is a key hallmark of the ageing process and ultimately leads to a variety of important age-related pathologies such as cancer, Parkinson’s disease and ...
Docherty, Craig K. +2 more
core +3 more sources
MicrobiologyOpen, 2020 Pyruvate dehydrogenase (PDH) and 2‐oxoglutarate dehydrogenase (ODH) are critical enzymes in central carbon metabolism. In Corynebacterium glutamicum, an unusual hybrid complex consisting of CgE1p (thiamine diphosphate‐dependent pyruvate dehydrogenase ...
Hirokazu Kinugawa +5 more
doaj +1 more source
A Metabolite Specific 3D Stack-of-Spiral bSSFP Sequence for Improved Lactate Imaging in Hyperpolarized [1-$^{13}$C]Pyruvate Studies on a 3T Clinical Scanner [PDF]
Magn Reson Med. 2020; 84: 1113-1125, 2020 Purpose: The balanced steady-state free precession sequence has been previously explored to improve the efficient use of non-recoverable hyperpolarized $^{13}$C magnetization, but suffers from poor spectral selectivity and long acquisition time. The purpose of this study was to develop a novel metabolite-specific 3D bSSFP ("MS-3DSSFP") sequence with ...
arxiv +1 more source
Molecular Architecture of Pyruvate Dehydrogenase Complexes [PDF]
Biophysical Journal, 2009 Pyruvate dehydrogenase multienzyme complexes are among the largest multifunctional protein assemblies within cells, catalyzing the four-step conversion of pyruvate to acetyl CoA using an assembly comprised of multiple copies of E1, E2 and E3 enzymes, Using cryo-electron microscopy, we have shown that the outer shell enzymes (E1 decarboxylase and E3 ...
openaire +2 more sources
Electron transport phosphorylation in rumen butyrivibrios: unprecedented ATP yield for glucose fermentation to butyrate. [PDF]
, 2015 From a genomic analysis of rumen butyrivibrios (Butyrivibrio and Pseudobutyrivibrio sp.), we have re-evaluated the contribution of electron transport phosphorylation (ETP) to ATP formation in this group.
Firkins, Jeffrey L, Hackmann, Timothy J
core +2 more sources
Relevance of electron spin dissipative processes to dynamic nuclear polarization via thermal mixing [PDF]
Phys. Chem. Chem. Phys. 16,753-764 (2014), 2013 The available theoretical approaches aiming at describing Dynamic Nuclear spin Polarization (DNP) in solutions containing molecules of biomedical interest and paramagnetic centers are not able to model the behaviour observed upon varying the concentration of trityl radicals or the polarization enhancement caused by moderate addition of gadolinium ...
arxiv +1 more source
Energy metabolism in human pluripotent stem cells and their differentiated counterparts [PDF]
, 2011 Background: Human pluripotent stem cells have the ability to generate all cell types present in the adult organism, therefore harboring great potential for the in vitro study of differentiation and for the development of cell-based therapies. Nonetheless
Easley IV, CA +7 more
core +10 more sources