Results 31 to 40 of about 80,914 (291)

A simple and reliable method for determination of optimum pH in coupled enzyme assays

BioTechniques, 2020
Determination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes.
Lee Bowman, Rachael Motamed, Paul Lee, Kadijah Aleem, Astha S Berawala, Katherine L Hayden, David J Bzik, Debasish Chattopadhyay   +7 more
doaj   +1 more source

Detecting de novo Hepatic Ketogenesis Using Hyperpolarized [2-13C] Pyruvate

Frontiers in Physiology, 2022
The role of ketones in metabolic health has progressed over the past two decades, moving from what was perceived as a simple byproduct of fatty acid oxidation to a central player in a multiplicity of disease states. Previous work with hyperpolarized (HP)
Mukundan Ragavan, Marc A. McLeod, Anna Rushin, Matthew E. Merritt   +3 more
doaj   +1 more source

Warburg effect hypothesis in autism Spectrum disorders

Molecular Brain, 2018
Autism spectrum disorder (ASD) is a neurodevelopmental disease which is characterized by a deficit in social interactions and communication with repetitive and restrictive behavior. In altered cells, metabolic enzymes are modified by the dysregulation of
Alexandre Vallée, Jean-Noël Vallée
doaj   +1 more source

Melatonin Therapy Modulates Cerebral Metabolism and Enhances Remyelination by Increasing PDK4 in a Mouse Model of Multiple Sclerosis

Frontiers in Pharmacology, 2019
Metabolic disturbances have been implicated in demyelinating diseases including multiple sclerosis (MS). Melatonin, a naturally occurring hormone, has emerged as a potent neuroprotective candidate to reduce myelin loss and improve MS outcomes.
Majid Ghareghani, Majid Ghareghani, Linda Scavo, Linda Scavo, Yahya Jand, Naser Farhadi, Hossein Sadeghi, Amir Ghanbari, Stefania Mondello, Stefania Mondello, Damien Arnoult, Sajjad Gharaghani, Kazem Zibara, Kazem Zibara   +13 more
doaj   +1 more source

Evidence that pyruvate dehydrogenase kinase belongs to the ATPase/kinase superfamily [PDF]

Biochemical Journal, 1999
In this study the roles of invariant Asn-247, Asp-282, Gly-284, Gly-286 and Gly-319 of pyruvate dehydrogenase kinase were investigated by site-directed mutagenesis. Recombinant kinases, wild-type, Asn-247Ala, Asp-282Ala, Gly-284Ala, Gly-286Ala and Gly-319Ala, were expressed in bacteria, purified, and characterized. Three mutant kinases, Asn-247Ala, Asp-
M, Bowker-Kinley, K M, Popov
openaire   +2 more sources

Modification of pyruvate kinase and lactate dehydrogenase in foot muscle of the sea mussel Mytilus galloprovincialis under anaerobiosis and recovery

Brazilian Journal of Medical and Biological Research, 1997
The modification of pyruvate kinase (PK) and lactate dehydrogenase (LDH) activity in foot muscle of the mussel Mytilus galloprovincialis during exposure to air and recovery in water was investigated.
V.I. Lushchak, T.V. Bahnjukova, A.V. Spichenkov   +2 more
doaj   +1 more source

PYRUVATE DEHYDROGENASE KINASE (PDK) [PDF]

, 2020
: Several oximes of triterpenes with a 17-~ hydroxyl and abietane derivatives are inlfibitors of pyruvate dehydrogenase kinase (PDK) activity. The oxime 12 and dehydroabietyl amine 2 exhibit a blood glucose lowering effect in the diabetic ob/ob mouse ...
Judit Koletar, Robert E Damon, Jiaping Gao, Christine C Vinluan, Thomas D Aicher, Suraj S Sherry, Leonard J Brand, Emma L Kaplarh, William R Mann   +8 more
core  

Malic Acid Production by Saccharomyces cerevisiae: Engineering of Pyruvate Carboxylation, Oxaloacetate Reduction, and Malate Export

, 2008
Malic acid is a potential biomass-derivable "building block" for chemical synthesis. Since wild-type Saccharomyces cerevisiae strains produce only low levels of malate, metabolic engineering is required to achieve efficient malate production with this ...
Van Maris, A.J.A. (author), Dijkema, C., Van Winden, W.A. (author), Winden, W.A., van, Dijkema, C. (author), Zelle, R.M. (author), Van Maris, A.J.A., Van Winden, W.A., De Hulster, E., Geertman, J.M., Pronk, J.T. (author), Hulster, E., de, Geertman, J.M.A., Winkler, A.A. (author), Van Dijken, J.P., Pronk, J.T., Winkler, A.A., De Hulster, E. (author), De Waard, P. (author), Geertman, J.M. (author), Waard, P., de, Zelle, R.M., De Waard, P., Van Dijken, J.P. (author)   +23 more
core   +1 more source

Fluorometric procedures for measuring triglyceride concentrations in small amounts of tissue and plasma.

Journal of Lipid Research, 1988
It has been previously shown that triglycerides can be specifically hydrolyzed by lipase from Rhizopus arrhizus in the presence of hog liver esterase and sodium dodecyl sulfate.
P M Nemeth, O E Hitchins, L Solanki, T G Cole   +3 more
doaj   +1 more source

Pyruvate dehydrogenase kinase inhibition: Reversing the Warburg effect in cancer therapy

, 2016
The poor efficacy of many cancer chemotherapeutics, which are often non-selective and highly toxic, is attributable to the remarkable heterogeneity and adaptability of cancer cells. The Warburg effect describes the up regulation of glycolysis as the main
Parkin, Edward Thomas; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster, Bell, Hayden; Division of Biomedical and Life Sciences, Faculty of Health and Medicine, Lancaster University, Lancaster   +1 more
core   +2 more sources