Results 1 to 10 of about 1,270,348 (335)

Research progress on the role of PKM2 in the immune response

Frontiers in Immunology, 2022
Pyruvate kinase (PK) is a key enzyme that catalyzes the dephosphorylation of phosphoenolpyruvate (PEP) into pyruvate, and is responsible for the production of ATP during glycolysis.
Chunyan Liu, Chenchen Liu, Rong Fu
doaj   +1 more source

Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23

Nature Communications, 2017
Tumour cells secrete exosomes that are involved in the remodelling of the tumour–stromal environment and promoting malignancy. The mechanisms governing tumour exosome release, however, remain incompletely understood.
Yao Wei, Dong Wang, Fangfang Jin, Zhen Bian, Limin Li, Hongwei Liang, Mingzhen Li, Lei Shi, Chaoyun Pan, Dihan Zhu, Xi Chen, G. Hu, Yuan Liu, Chen-Yu Zhang, Ke Zen   +14 more
semanticscholar   +1 more source

Novel mutations associated with pyruvate kinase deficiency in Brazil

Hematology, Transfusion and Cell Therapy, 2018
Background: Pyruvate kinase deficiency is a hereditary disease that affects the glycolytic pathway of the red blood cell, causing nonspherocytic hemolytic anemia.
Maria Carolina Costa Melo Svidnicki, Andrey Santos, Jhonathan Angel Araujo Fernandez, Ana Paula Hitomi Yokoyama, Isis Quezado Magalhães, Vitoria Regia Pereira Pinheiro, Silvia Regina Brandalise, Paulo Augusto Achucarro Silveira, Fernando Ferreira Costa, Sara Teresinha Olalla Saad   +9 more
doaj   +1 more source

A simple and reliable method for determination of optimum pH in coupled enzyme assays

BioTechniques, 2020
Determination of the optimum pH in a coupled enzyme assay poses significant challenges because altering the pH of the reaction mixture can affect the performance of both enzymes.
Lee Bowman, Rachael Motamed, Paul Lee, Kadijah Aleem, Astha S Berawala, Katherine L Hayden, David J Bzik, Debasish Chattopadhyay   +7 more
doaj   +1 more source

Natural Product Micheliolide (MCL) Irreversibly Activates Pyruvate Kinase M2 and Suppresses Leukemia

Journal of Medicinal Chemistry, 2018
Metabolic reprogramming of cancer cells is essential for tumorigenesis in which pyruvate kinase M2 (PKM2), the low activity isoform of pyruvate kinase, plays a critical role. Herein, we describe the identification of a nature-product-derived micheliolide
Jing Li, Shanshan Li, Jianshuang Guo, Qiu-ying Li, Jing Long, Cheng Ma, Yahui Ding, Chunli Yan, Liangwei Li, Zhigang Wu, He Zhu, K. Li, Liuqing Wen, Quan Zhang, Qingqing Xue, Caili Zhao, Ning Liu, I. Ivanov, M. Luo, Rimo Xi, H. Long, P. Wang, Yue-hua Chen   +22 more
semanticscholar   +1 more source

Pyruvate kinase M2 expression, but not pyruvate kinase activity, is up-regulated in a grade-specific manner in human glioma. [PDF]

PLoS ONE, 2013
Normal tissues express the M1 isoform of pyruvate kinase (PK) that helps generate and funnel pyruvate into the mitochondria for ATP production. Tumors, in contrast, express the less active PKM2 isoform, which limits pyruvate production and spares ...
Joydeep Mukherjee, Joanna J Phillips, Shichun Zheng, John Wiencke, Sabrina M Ronen, Russell O Pieper   +5 more
doaj   +1 more source

Erythrocytic Pyruvate Kinase and Malondialdehyde Levels in Acute Leukaemia Patients [PDF]

Journal of Clinical and Diagnostic Research, 2012
Introduction: Many acquired enzymopathies have been reported in patients with acute leukaemia. Oxidative stress is also found to be increased in acute leukaemia, which can be indicated by the increased malondialdehyde (MDA) levels.
Veena Singh, Munish Kaushik, Kiran Dahiya, P.S. Ghalaut, Rakesh Dhankhar, Ragini Singh   +5 more
doaj  

Addressing the diagnostic gaps in pyruvate kinase deficiency: Consensus recommendations on the diagnosis of pyruvate kinase deficiency

American journal of hematology/oncology, 2018
Pyruvate kinase deficiency (PKD) is the most common enzyme defect of glycolysis and an important cause of hereditary, nonspherocytic hemolytic anemia.
P. Bianchi, E. Fermo, B. Glader, H. Kanno, A. Agarwal, W. Barcellini, S. Eber, J. Hoyer, D. Kuter, T. Maia, M. Mañú-Pereira, T. Kalfa, S. Pissard, J. Segovia, E. V. van Beers, P. Gallagher, D. Rees, R. van Wijk   +17 more
semanticscholar   +1 more source

Endothelial pyruvate kinase M2 maintains vascular integrity

Journal of Clinical Investigation, 2018
The M2 isoform of pyruvate kinase (PKM2) is highly expressed in most cancer cells, and has been studied extensively as a driver of oncogenic metabolism. In contrast, the role of PKM2 in nontransformed cells is little studied, and nearly nothing is known ...
Boa Kim, Cholsoon Jang, Harita Dharaneeswaran, Jian Li, Mohit Bhide, S.T. Yang, Kristina Li, Z. Arany   +7 more
semanticscholar   +1 more source

Butyrate Suppresses the Proliferation of Colorectal Cancer Cells via Targeting Pyruvate Kinase M2 and Metabolic Reprogramming *

Molecular & Cellular Proteomics, 2018
Butyrate is a short chain fatty acid present in a high concentration in the gut lumen. It has been well documented that butyrate, by serving as an energetic metabolite, promotes the proliferation of normal colonocytes while, by serving as a histone ...
Qing-ran Li, L. Cao, Yang Tian, Pei Zhang, Chujie Ding, Wenjie Lu, Chenxi Jia, Chang Shao, Wen-yue Liu, Dong Wang, Hui Ye, H. Hao   +11 more
semanticscholar   +1 more source