Results 61 to 70 of about 68,490 (304)

Pyruvate dehydrogenase kinase regulates hepatitis C virus replication [PDF]

Scientific Reports, 2016
AbstractDuring replication, hepatitis C virus (HCV) utilizes macromolecules produced by its host cell. This process requires host cellular metabolic reprogramming to favor elevated levels of aerobic glycolysis. Therefore, we evaluated whether pyruvate dehydrogenase kinase (PDK), a mitochondrial enzyme that promotes aerobic glycolysis, can regulate HCV ...
Yu Na Kang, Soo-Young Park, Yeon-Kyung Choi, Yeon-Kyung Choi, Keun-Gyu Park, Keun-Gyu Park, Jun-Kyu Byun, Gwon-Soo Jung, Gwon-Soo Jung, Eui-Cheol Shin, Mi-Kyung Kim, Sungwoo Lee, Jae-Han Jeon, Jae-Han Jeon, Sung Woo Kim, Sung Woo Kim, Se Young Jang, Inkyu Lee, Inkyu Lee   +18 more
openaire   +4 more sources

PFKM‐Driven Lactate Overproduction Promotes Atrial Fibrillation via Triggering Cardiac Fibroblasts Histone Lactylation

Advanced Science, EarlyView.
The upregulation of PFKM activates glycolysis, thereby enhancing lactate production in atrial tissue. Atrial lactate accumulation promotes global lactylation and H3K18 lactylation in atrial fibroblasts. P300‐mediated H3K18 lactylation up‐regulates TGF‐β1 transcription, leading to fibroblast activation and thereby contributing to atrial fibrosis.
Ning Fang, Ning Zhang, Xiaohui Jiang, Sen Yan, Zhiqi Wang, Qianhui Gao, Mingcheng Xu, Lin Mu, Xiaoming Li, Jiuling Chen, Song Zhang, Yu Duan, Fengxiang Yun, Luyifei Li, Yun Zhang, Yongtai Gong   +15 more
wiley   +1 more source

Effects of alpha-ketoisovalerate on bovine heart pyruvate dehydrogenase complex and pyruvate dehydrogenase kinase.

Journal of Biological Chemistry, 1986
The regulatory effects of alpha-ketoisovalerate on purified bovine heart pyruvate dehydrogenase complex and endogenous pyruvate dehydrogenase kinase were investigated. Incubation of pyruvate dehydrogenase complex with 0.125 to 10 mM alpha-ketoisovalerate caused an initial lag in enzymatic activity, followed by a more linear but inhibited rate of NADH ...
J G Robertson, Merle S. Olson, L L Barron   +2 more
openaire   +3 more sources

Pyruvate Dehydrogenase Kinase 4 [PDF]

Diabetes, 2008
OBJECTIVE—Pyruvate dehydrogenase complex (PDC) serves as the metabolic switch between glucose and fatty acid utilization. PDC activity is inhibited by PDC kinase (PDK). PDC shares the same substrate, i.e., pyruvate, as glyceroneogenesis, a pathway controlling fatty acid release from white adipose tissue (WAT).
Françoise Fouque, Jean-Marc Blouin, Emilie Distel, Thomas Cadoudal, Sylvie Durant, Claude Forest, Chantal Benelli, Sylvie Bortoli, Martine Collinet   +8 more
openaire   +2 more sources

Nanomedicine‐Driven Modulation of Reductive Stress for Cancer Therapy

Advanced Science, EarlyView.
This article introduces a novel therapeutic paradigm that deliberately induces reductive stress within tumor cells to exploit their unique biochemical vulnerabilities. It systematically reviews the underlying biological mechanisms, current detection and monitoring techniques, and emerging advanced nanotechnology platforms specifically designed to ...
Yumin Mao, Xianping Liu, Xu Chu, Feixiang Chen, Ruicheng Shi, Zonghao Liu, Yelin Wu, Yanyan Liu, Wenbo Bu   +8 more
wiley   +1 more source

Essential role of coenzyme A in pyruvate dehydrogenase kinase activity

FEBS Letters, 1982
The rate of phosphorylation and concomitant inactivation of purified pig heart muscle pyruvate dehydrogenase complex by intrinsic kinase (EC 2.7.1.99) is markedly accelerated by the addition of coenzyme A to the incubation medium, showing a half‐maximum effect at 1.8 μM. The pantetheine moiety is the effective part of the coenzyme A molecule.
O.H. Weiland, E.A. Siess
openaire   +3 more sources

Mitochondrial Regulation of CD8⁺ T Cells: Mechanisms and Therapeutic Modulation

Advanced Science, EarlyView.
This article comprehensively explores the pivotal role of mitochondrial function in CD8⁺ T cell immunotherapy. It highlights how mitochondrial dynamics regulate T cell activation, differentiation, and persistence, and how mitochondrial dysfunction leads to immune exhaustion and impaired antitumor/antiviral responses.
Xu Chen, Pei Lin, Ye Lu, Jiarong Zheng, Yunfan Lin, Zihao Zhou, Li Cui, Xinyuan Zhao   +7 more
wiley   +1 more source

HPD is an RNA‐Binding Protein Sustaining Ovarian Cancer Cell Glycolysis, Tumor Growth, and Drug Resistance

Advanced Science, EarlyView.
HPD is identified as an RNA‐binding protein that promotes mRNA translation by binding to RRACH motifs via its double‐stranded RNA‐binding domains. This RNA‐binding activity critically sustains glycolysis in ovarian cancer cells. Disrupting HPD's RNA‐binding function effectively suppresses tumor growth and enhances therapeutic sensitivity, highlighting ...
Fei Xie, Han Zhang, Xintong Dai, Mengxin Tu, Chenxi Yu, Lumeng Liu, Yajuan Guo, Huanran Sun, Qingle Gao, Jiyan Wang, Mingming Sun, Qijun Zhang, Taoyuan Wang, Tao He, Zhen Li, Yanping Li, Tao Wang, Jianguo Zhao, Zhongjie Chen, Chunze Zhang, Shuai Zhang, Changliang Shan   +21 more
wiley   +1 more source

PDK4 dictates metabolic resistance to ferroptosis by suppressing pyruvate oxidation and fatty acid synthesis

Cell Reports, 2021
Summary: Although induction of ferroptosis, an iron-dependent form of non-apoptotic cell death, has emerged as an anticancer strategy, the metabolic basis of ferroptotic death remains poorly elucidated.
Xinxin Song, Jiao Liu, Feimei Kuang, Xin Chen, Herbert J. Zeh, III, Rui Kang, Guido Kroemer, Yangchun Xie, Daolin Tang   +8 more
doaj  

Effect of dexamethasone on gluconeogenesis, pyruvate kinase, pyruvate carboxylase and pyruvate dehydrogenase flux in isolated hepatocytes [PDF]

Biochemical Journal, 1993
Treatment of 18 h-starved rats with dexamethasone and subsequent isolation and incubation of the hepatocytes in the presence of the steroid increased gluconeogenic flux with both 1.0 mM pyruvate and 1.0 mM lactate plus 0.2 mM pyruvate as the substrate. The magnitude of stimulation was comparable with both substrates.
Claire G. Jones, S K Hothi, Michael A. Titheradge   +2 more
openaire   +3 more sources