Results 11 to 20 of about 1,004,764 (341)

Brief guide to RT-qPCR

Molecules and Cells
RNA quantification is crucial for understanding gene expression and regulation. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a widely used technique for RNA quantification because of its practical and quantitative nature, sensitivity, and specificity.
Bong, Dajeong, Sohn, Jooyeon, Lee, Seung-Jae V.   +2 more
openaire   +3 more sources

A beginner’s guide to RT-PCR, qPCR and RT-qPCR [PDF]

The Biochemist, 2020
The development of the polymerase chain reaction (PCR), for which Kary Mullis received the 1992 Novel Prize in Chemistry, revolutionized molecular biology. At around the time that prize was awarded, research was being carried out by Russel Higuchi which led to the discovery that PCR can be monitored using fluorescent probes, facilitating quantitative ...
Grace E. Adams
openaire   +2 more sources

Fast SARS-CoV-2 detection by RT-qPCR in preheated nasopharyngeal swab samples [PDF]

International Journal of Infectious Diseases, 2020
Objectives The gold-standard COVID-19 diagnosis relies on detecting SARS-CoV-2 using RNA purification and one-step retrotranscription and quantitative PCR (RT-qPCR).
Julia Alcoba-Flórez, Rafaela González‐Montelongo, Antonio Íñigo-Campos, Diego García-Martínez de Artola, Helena Gil-Campesino, Laura Ciuffreda, Agustı́n Valenzuela-Fernández, Carlos Flores, Carlos Flores   +8 more
openalex   +2 more sources

The Environmental Microbiology Minimum Information (EMMI) Guidelines: qPCR and dPCR Quality and Reporting for Environmental Microbiology.

Environmental Science and Technology, 2021
Real-time quantitative polymerase chain reaction (qPCR) and digital PCR (dPCR) methods have revolutionized environmental microbiology, yielding quantitative organism-specific data of nucleic acid targets in the environment.
M. Borchardt, A. Boehm, M. Salit, S. Spencer, K. Wigginton, R. Noble   +5 more
semanticscholar   +1 more source

Comparison of RT-qPCR and RT-dPCR Platforms for the Trace Detection of SARS-CoV-2 RNA in Wastewater

ACS ES&T water, 2022
We compared reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and RT digital PCR (RT-dPCR) platforms for the trace detection of SARS-CoV-2 RNA in low-prevalence COVID-19 locations in Queensland, Australia, using CDC N1 and CDC N2 ...
W. Ahmed, W. Smith, S. Metcalfe, Greg Jackson, Phil M. Choi, Mary Morrison, Daniel Field, P. Gyawali, A. Bivins, K. Bibby, S. Simpson   +10 more
semanticscholar   +1 more source

Correlation between Targeted qPCR Assays and Untargeted DNA Shotgun Metagenomic Sequencing for Assessing the Fecal Microbiota in Dogs

Animals, 2023
Simple Summary Untargeted shotgun DNA sequencing of fecal samples is a relatively novel approach to study the microbiome. This method allows better classification of bacteria on a species level compared to traditional 16S rRNA gene sequencing, and ...
Chi-Hsuan Sung, R. Pilla, Chih-Chun Chen, P. Ishii, L. Toresson, Karin Allenspach-Jorn, A. Jergens, S. Summers, K. Swanson, H. Volk, Teresa Schmidt, Helene Stuebing, J. Rieder, K. Busch, Melanie Werner, Anja Lisjak, F. Gaschen, Sara E Belchik, M. Tolbert, J. Lidbury, J. Steiner, J. Suchodolski   +21 more
semanticscholar   +1 more source

Multiplex qPCR discriminates variants of concern to enhance global surveillance of SARS-CoV-2

PLoS Biology, 2021
With the emergence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) variants that may increase transmissibility and/or cause escape from immune responses, there is an urgent need for the targeted surveillance of circulating lineages.
C. Vogels, M. Breban, I. Ott, T. Alpert, M. Petrone, A. Watkins, C. Kalinich, Rebecca Earnest, Jessica E. Rothman, J. Goes de Jesus, Ingra Morales Claro, Giulia Magalhães Ferreira, M. Crispim, L. Singh, H. Tegally, U. J. Anyaneji, E. Hodcroft, C. Mason, G. Khullar, Jessica Metti, J. Dudley, M. Mackay, Megan Nash, Jianhui Wang, Chen Liu, P. Hui, S. Murphy, C. Neal, E. Laszlo, M. Landry, A. Muyombwe, R. Downing, J. Razeq, Túlio de Oliveira, Nuno R. Faria, E. Sabino, R. Neher, J. Fauver, N. Grubaugh   +38 more
semanticscholar   +1 more source

Evaluation of COVID-19 RT-qPCR test in multi-sample pools

medRxiv, 2020
The recent emergence of SARS-CoV-2 lead to a current pandemic of unprecedented levels. Though diagnostic tests are fundamental to the ability to detect and respond, many health systems are already experiencing shortages of reagents associated with this ...
I. Yelin, N. Aharony, E. Tamar, Amir Argoetti, Esther Messer, Dina Berenbaum, Einat Shafran, Areen Kuzli, Nagham Gandali, Omer Shkedi, T. Hashimshony, Y. Mandel-Gutfreund, M. Halberthal, Y. Geffen, M. Szwarcwort-Cohen, R. Kishony   +15 more
semanticscholar   +1 more source

Web-based LinRegPCR: application for the visualization and analysis of (RT)-qPCR amplification and melting data

BMC Bioinformatics, 2021
The analyses of amplification and melting curves have been shown to provide valuable information on the quality of the individual reactions in quantitative PCR (qPCR) experiments and to result in more reliable and reproducible quantitative results.
A. Untergasser, J. Ruijter, V. Beneš, M. V. D. van den Hoff   +3 more
semanticscholar   +1 more source

Combination of RT‐qPCR testing and clinical features for diagnosis of COVID‐19 facilitates management of SARS‐CoV‐2 outbreak

Journal of Medical Virology, 2020
In December 2019, a cluster of acute respiratory illness occurred in Wuhan, Hubei Province, China. This disease is now officially known as 2019 novel coronavirus disease (COVID-19) from WHO, novel coronavirus pneumonia This article is protected by ...
Yishan Wang, Hanyujie Kang, Xuefeng Liu, Z. Tong   +3 more
semanticscholar   +1 more source