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MNAzyme qPCR: A Superior Tool for Multiplex qPCR
2013Multicomponent nucleic acid enzymes (MNAzymes) are nucleic acid enzymes composed of multiple oligonucleotide partzymes that only associate to form catalytic complexes in the presence of a target nucleic acid. Once assembled, MNAzymes cleave a separate substrate (probe) between fluorophore and quencher labels to produce a fluorescent signal indicative ...
Elisa, Mokany, Alison V, Todd
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qPCR Genotyping of Polyploid Species
2023A simple and cost-effective method for genotyping polyploid plants using quantitative PCR (qPCR) is described in this chapter. There is no additional operation, only simultaneous amplification of alleles and reference sequences with constant copy number in the genome.
Haiyan, Wang +3 more
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qPCR Detection of Candida auris Using the GPS™ CanAur MONODOSE dtec–qPCR Test
2022Candida auris is a multidrug-resistant pathogenic ascomycete yeast of increasing health concern. C. auris colonizes patient's skin and can persist for weeks on surfaces, so it can be transmitted within and between hospitals. The most common diagnostic platforms in microbiology use reference databases that have not yet incorporated C.
Antonio, Martínez-Murcia +2 more
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Measuring RNAi Knockdown using qPCR
2013We describe a scalable method for measuring changes in gene expression following RNAi in mammalian cells. This protocol outlines methods to transfect cells, isolate total RNA, perform cDNA synthesis, run qPCR reactions with multiplexed TaqMan dual hydrolysis probes, and analyze the results from qPCR using relative quantification (Fleige et al., 2006 ...
Stuart, Milstein +3 more
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