Results 101 to 110 of about 1,617,447 (322)

Padlock probe-mediated qRT-PCR for DNA computing answer determination [PDF]

Natural Computing, 2010
Padlock probe-mediated quantitative real time PCR (PLP-qRT-PCR) was adapted to quantify the abundance of sequential 10mer DNA sequences for use in DNA computing to identify optimal answers of traveling salesman problems. The protocol involves: (i) hybridization of a linear PLP with a target DNA sequence; (ii) PLP circularization through enzymatic ...
Fusheng, Xiong, Wayne D, Frasch
openaire   +2 more sources

Improving PARP inhibitor efficacy in bladder cancer without genetic BRCAness by combination with PLX51107

Molecular Oncology, EarlyView.
Clinical trials on PARP inhibitors in urothelial carcinoma (UC) showed limited efficacy and a lack of predictive biomarkers. We propose SLFN5, SLFN11, and OAS1 as UC‐specific response predictors. We suggest Talazoparib as the better PARP inhibitor for UC than Olaparib.
Jutta Schmitz, Anna L. Bartkowiak, Michael Rose, Nora Kolks, Patrick Petzsch, Vandana Solanki, Anne Stoffel, Bianca Faßbender, Leandra Lepping, Julka Volkamer, Karl Köhrer, Marc Seifert, Tokameh Mahmoudi, Tahlita C. M. Zuiverloon, Günter Niegisch, Michèle J. Hoffmann   +15 more
wiley   +1 more source

Screening for qRT-PCR Internal Reference Genes in Clarias fuscus

Progress in Fishery Sciences
Quantitative real-time polymerase chain reaction (qRT-PCR) is one of the most widely used molecular techniques, which allows the detection and quantification of gene expression due to its high sensitivity, specificity, and reproducibility.
Yijun SHEN, Yian ZHU, Zhihua YANG, Minghui YE, Dayan ZHOU, Guangli LI, Changxu TIAN   +6 more
doaj   +1 more source

Phytochrome activates the plastid-encoded RNA polymerase for chloroplast biogenesis via nucleus-to-plastid signaling. [PDF]

, 2019
Light initiates chloroplast biogenesis by activating photosynthesis-associated genes encoded by not only the nuclear but also the plastidial genome, but how photoreceptors control plastidial gene expression remains enigmatic.
Blaha, Gregor M, Cao, Jun, Chen, Meng, Pasoreck, Elise K, Wang, He, Weigel, Detlef, Yoo, Chan Yul   +6 more
core   +2 more sources

COVID‐19 diagnostics for resource‐limited settings: Evaluation of “unextracted” qRT‐PCR

Journal of Medical Virology, 2020
The coronavirus disease 2019 (COVID‐19) pandemic has created a precipitous increase in the need for molecular diagnostics. Unfortunately, access to RNA extraction reagents can represent a bottleneck for quantitative real‐time reverse transcriptase ...
N. M. Adams, M. Leelawong, Alison Benton, Criziel D. Quinn, F. Haselton, Jonathan E. Schmitz   +5 more
semanticscholar   +1 more source

Modeling qRT-PCR dynamics with application to cancer biomarker quantification [PDF]

Statistical Methods in Medical Research, 2017
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is widely used for molecular diagnostics and evaluating prognosis in cancer. The utility of mRNA expression biomarkers relies heavily on the accuracy and precision of quantification, which is still challenging for low abundance transcripts.
Inna, Chervoneva, Boris, Freydin, Terry, Hyslop, Scott A, Waldman   +3 more
openaire   +2 more sources

Class IIa HDACs forced degradation allows resensitization of oxaliplatin‐resistant FBXW7‐mutated colorectal cancer

Molecular Oncology, EarlyView.
HDAC4 is degraded by the E3 ligase FBXW7. In colorectal cancer, FBXW7 mutations prevent HDAC4 degradation, leading to oxaliplatin resistance. Forced degradation of HDAC4 using a PROTAC compound restores drug sensitivity by resetting the super‐enhancer landscape, reprogramming the epigenetic state of FBXW7‐mutated cells to resemble oxaliplatin ...
Vanessa Tolotto, Nicolò Gualandi, Ylenia Cortolezzis, Raffaella Picco, Monica Colitti, Francesca D'Este, Mariachiara Gani, Wayne W. Hancock, Giovanni Terrosu, Cristina Degrassi, Francesca Agostini, Claudio Brancolini, Luigi E. Xodo, Eros Di Giorgio   +13 more
wiley   +1 more source

Identification of early gene expression changes in primary cultured neurons treated with topoisomerase I poisons. [PDF]

, 2016
Topoisomerase 1 (TOP1) poisons like camptothecin (CPT) are currently used in cancer chemotherapy but these compounds can have damaging, off-target effects on neurons leading to cognitive, sensory and motor deficits.
Butchbach, Matthew E.R., Gentillon, Cinsley, Harris, Ashlee W., Holbrook, Jennifer, Lumpkin, Casey J., McCahan, Suzanne M., Rossi, Sharyn L., Wang, Wenlan   +7 more
core   +2 more sources

Therapeutic strategies for MMAE‐resistant bladder cancer through DPP4 inhibition

Molecular Oncology, EarlyView.
We established monomethyl auristatin E (MMAE)‐resistant bladder cancer (BC) cell lines by exposure to progressively increasing concentrations of MMAE in vitro. RNA sequencing showed DPP4 expression was increased in MMAE‐resistant BC cells. Both si‐DPP4 and the DPP4 inhibitor sitagliptin suppressed the viability of MMAE‐resistant BC cells.
Gang Li, Shuichi Tatarano, Hirofumi Yoshino, Saeki Saito, Mitsuhiko Tominaga, Junya Arima, Ikumi Fukuda, Takashi Sakaguchi, Ryosuke Matsushita, Yasutoshi Yamada, Hideki Enokida   +10 more
wiley   +1 more source

A conserved phosphorylation site regulates the transcriptional function of ETHYLENE-INSENSITIVE3-like1 in tomato [PDF]

, 2011
ETHYLENE-INSENSITIVE3/ETHYLENE-INSENSITIVE3-like (EIN3/EIL) transcription factors are important downstream components of the ethylene transduction pathway known to regulate the transcription of early ethylene-responsive genes in plants.
Abel, Alonso, Chang, Chao, Fan, Fryer, Gerber, Girod, Guo, Hellens, J. Li, Kammann, Kieber, Kim, Klimczak, Kosugi, L. Tang, Lehman, M. Bouzayen, M. Zouine, Mishra, Montgomery, Raz, Riechmann, Riechmann, Shaner, Sharma, Solano, Stepanova, Tieman, Y. Yang, Yamamoto, Yamasaki, Yokotani, Yoo, Z. Li, Zhou, Zhuang   +37 more
core   +2 more sources