Results 101 to 110 of about 1,108,829 (262)

Single‐Cell Dissection of Therapy‐Induced Remodeling Uncovers a Fibroblast‐Driven Immunosuppressive Niche and Targetable Vulnerabilities in Lethal Prostate Cancer

Advanced Science, EarlyView.
Single‐cell longitudinal profiling reveals that androgen‐deprivation therapy induces a DPT+ fibroblast‐complement axis that suppresses macrophage inflammation and drives CD8+ T cell exhaustion in prostate cancer. Concurrently, resistant epithelial subpopulations persist and engage TSPAN1‐ and NRXN1‐mediated programs promoting CRPC and neuroendocrine ...
Yang Chen, Dandan Dong, Jinling Liao, Naikai Liao, Mengqi Liu, Qin Zhang, Siting Chen, Qi Liu, Ying Lu, Tianyu Li, Chunlin Zou, Qiong Song, Qiuyan Wang, Jian Liang, Qiji Xie, Chengyang Li, Mengyun Wang, Jiwen Cheng, Zengnan Mo, Gong‐Hong Wei   +19 more
wiley   +1 more source

Evaluation of the effects of tropical tanniferous plants on rumen microbiota using qRT PCR and DGGE analysis

Czech Journal of Animal Science, 2013
Tanniferous forages may have bacteriostatic and/or bactericidal effect on different rumen microbial populations. We investigated the influence of the tropical tanniferous plants Styzolobium aterrimum (STA), Styzolobium deeringianum (STD), Leucaena ...
C. Longo, A.L. Abdalla, J. Liebich, I. Janzik, J. Hummel, P.S. Correa, K.-H. Südekum, P. Burauel   +7 more
doaj   +1 more source

Primer and probe sequences for qRT-PCR

, 2016
Primer and probe sequences for qRT ...
Helena J. Maier (3043422), Mark Fife (3043425), Ambalika Batra (3043431), William Mwangi (3043428), Colin Butter (104550), John R. Young (104544), Karen Staines (104539), Steven Van Borm (313768)   +7 more
core   +1 more source

TRAIP Mediates Alcohol‐Induced Liver Injury through Regulating β‐catenin Ubiquitin Degradation via Direct Interaction

Advanced Science, EarlyView.
This study reveals that NF‐κB1‐driven TRAIP upregulation in ALD correlates with disease severity. Mechanistically, TRAIP directly binds β‐catenin via its CC domain and promotes its K48‐linked ubiquitination and degradation, which is independent of the GSK3β/β‐TrCP pathway.
Zhan Wu, Mingjiang Liu, Lijuan Liao, Zhaohong Mo, Wenli Xu, Linfeng Mao, Yubing Chen, Qiuli Xie, Ming Yao, Shilian Chen, Stephen Tomlinson, Sebastian Mueller, Songqing He, Guandou Yuan   +13 more
wiley   +1 more source

qRT-PCR confirmation of microarray-based transcriptomic results.

, 2016
qRT-PCR confirmation of microarray-based transcriptomic results.
Mark J. Longwell (191416), J. Christopher Post (60144), Fen Z. Hu (60141), Garth D. Ehrlich (60143), Evan Powell (121249), Benjamin A. Janto (640669), N. Luisa Hiller (48998), Joshua C. Mell (350197), Rory A. Eutsey (191404), Barbara Byers (3179388), Todd Hillman (82333), Tenisha Blackwell (3179385), Jennifer M. Kress-Bennett (3179382)   +12 more
core   +1 more source

Co‐Delivery of Sustained Release Chondroitinase ABC‐37 With Human iPSC‐Derived Neural Progenitors Promotes Transplant Survival and Functional Recovery in a Rodent Model of Stroke

Advanced Science, EarlyView.
A regenerative strategy for stroke combines human stem cell–derived neural progenitor cells with sustained release of a matrix‐modifying, thermostable chondroitinase ABC‐37 enzyme. In a rat model of stroke, co‐delivery enhanced transplanted cell survival and neuronal differentiation, degraded inhibitory extracellular matrix components, and improved ...
Nitzan Letko Khait, David Xinzheyang Li, Keyi Guo, Hong Cui, Quinton Sirianni, Dhana Abdo, Cindi M. Morshead, Molly S. Shoichet   +7 more
wiley   +1 more source

Assessment of suitable reference genes for qRT-PCR analysis in Adelphocoris suturalis

Journal of Integrative Agriculture, 2018
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the most commonly-used tool for measurement of gene expression, but its accuracy and reliability depend on appropriate data normalization with the use of one or more stable ...
Jing LUO, Chao MA, Zhe LI, Bang-qin ZHU, Jiang ZHANG, Chao-liang LEI, Shuang-xia JIN, J. Joe Hull, Li-zhen CHEN   +8 more
doaj   +1 more source

GHRHR Deficiency Enhances Retinal Ganglion Cell Survival and Visual Functions in Experimental Glaucoma by Inhibiting Ferroptosis

Advanced Science, EarlyView.
Glaucoma, a major cause of blindness, involves retinal ganglion cell (RGC) degeneration. This study shows growth hormone‐releasing hormone receptor (GHRHR) deficiency preserves RGC survival and restores vision, unlike activation which only aids survival.
Yan Tong, Ming Ho Yam, Jiaxin Zhang, Lin Du, Linbin Zhou, Yolanda Wong Ying Yip, Bo Man Ho, Hemlata Bisnauthsing, Jiahui Li, Ivan Kong, Shushu Xu, Changzhen Fu, Karl K.H. So, Joaquim S.L. Vong, Ling‐Ping Cen, Ming‐Ming Yang, Khazeema Yousaf, Mai Har Sham, Sun On Chan, Poemen P. Chan, Chi Pui Pang, Clement C. Tham, Jing Na He, Jian Li, Wai Kit Chu   +24 more
wiley   +1 more source

Integrative Multi‐Omics Analysis Reveals a Mitochondrial–Immune Axis Associated With Neoadjuvant Chemotherapy Response in High‐Grade Serous Ovarian Cancer

Advanced Science, EarlyView.
Integrative multi‐omics analysis delineates a mitochondrial–immune axis governing neoadjuvant chemotherapy response in high‐grade serous ovarian cancer. Immune‐active tumors exhibit enhanced B‐cell infiltration and favorable sensitivity, whereas metabolically rewired tumors display oxidative phosphorylation dependency and resistance.
Wei Jiang, Yi Wang, Xiaohang Lu, Qianlan Yao, Libing Xiang, Xu Cai, Xiaoyu Tu, Shaoxian Tang, Ying Xu, Yufan Cheng, Liming Shen, Huijuan Yang   +11 more
wiley   +1 more source

qRT-PCR validation of select genes in control and treated plants.

, 2018
The grey-scale bars represent relative gene expression in control (dark grey) and treated plants (light grey) by qRT-PCR analysis (right y-axis). qRT-PCR data show the average relative expression of two biological samples with three technical replicates ...
Sebastian Reyes-Chin-Wo (281014), Amit Dhingra (93307), María José Rubio-Cabetas (5848841), Beatriz Bielsa (5848835), Seanna Hewitt (5848838)   +4 more
core   +1 more source