Results 151 to 160 of about 1,617,447 (322)

qRT-PCR primers used in this study

, 2019
qRT-PCR primers used in transcriptomic profiling for stress of low-temperature gradients in cotton (Gossypium hirsutum)
openaire   +2 more sources

Dengue Virus NS1 Protein as a Diagnostic Marker: Commercially Available ELISA and Comparison to qRT-PCR and Serological Diagnostic Assays Currently Used by the State of Florida

Journal of Tropical Medicine, 2017
Background. The proper management of patients infected with dengue virus requires early detection. Here, real-time molecular assays have proven useful but have limitations, whereas ELISAs that detect antibodies are still favored but results are obtained ...
Jason H. Ambrose, Shamala Devi Sekaran, Azliyati Azizan   +2 more
doaj   +1 more source

Assessment of suitable reference genes for qRT-PCR analysis in Adelphocoris suturalis

Journal of Integrative Agriculture, 2018
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is the most commonly-used tool for measurement of gene expression, but its accuracy and reliability depend on appropriate data normalization with the use of one or more stable ...
Jing LUO, Chao MA, Zhe LI, Bang-qin ZHU, Jiang ZHANG, Chao-liang LEI, Shuang-xia JIN, J. Joe Hull, Li-zhen CHEN   +8 more
doaj   +1 more source

Bone growth following demineralized bone matrix implantation requires angiogenesis [PDF]

, 2016
Angiogenesis is required for endochondral ossification during development and fracture healing; however the exact mechanisms and temporal relationship between the two processes remains unclear.
Lam, Stephanie
core  

Evaluation of suitable reference genes for qRT-PCR normalization in strawberry (Fragaria × ananassa) under different experimental conditions

BMC Molecular Biology, 2018
BackgroundStrawberry has received much attention due to its nutritional value, unique flavor, and attractive appearance. The availability of the whole genome sequence and multiple transcriptome databases allows the great possibility to explore gene ...
Yunting Zhang, Xiao-Xi Peng, Yi Liu, Yali Li, Ya Luo, Xiaorong Wang, Haoru Tang   +6 more
semanticscholar   +1 more source

RNA isolation and qRT-PCR v1

This protocol describes the procedure for RNA isolation, cDNA synthesis and qRT-PCR. Protocol overview A. RNA isolation and cDNA synthesis B. qRT-PCR qRT-PCR primer sequence table
Khaja Mohieddin Syed, Dirk Hockemeyer
openaire   +1 more source

Nano‐ and Micro‐Sized Solid Materials Used as Antiviral Agents

Advanced Functional Materials, EarlyView.
Due to the rise of viral infections in humans and possible viral outbreaks, the use of nano‐ or micro‐sized materials as antiviral agents is rapidly increasing. This review explores their antiviral properties against RNA and DNA viruses, either as a prevention or a treatment tool, by delving into their mechanisms of action and how to properly assess ...
Orfeas‐Evangelos Plastiras, Imelda Raczkiewicz, Peggy Bouquet, Jean‐Philippe Dacquin, Jeremy Dhainaut, Anne Goffard, Christophe Volkringer   +6 more
wiley   +1 more source

Reference gene selection for qRT-PCR analysis of flower development in Lagerstroemia indica and L. speciosa

PLoS ONE, 2018
Quantitative real-time polymerase chain reaction (qRT-PCR) is a prevalent method for gene expression analysis, depending on the stability of the reference genes for data normalization. Lagerstroemia indica and L.
Tangchun Zheng, Zhilin Chen, Yiqian Ju, Han Zhang, Ming Cai, H. Pan, Qixiang Zhang   +6 more
semanticscholar   +1 more source

Reverse transcription and qRT-PCR v1

, 2018

openaire   +1 more source

A Human Kidney Tubuloid Model of Repeated Cisplatin‐Induced Cellular Senescence and Fibrosis for Drug Screening

Advanced Healthcare Materials, EarlyView.
Replicating aging and senescence‐related pathophysiological responses in kidney organoids remains a significant challenge. Human adult renal tubular organoid, tubuloids, are successfully developed recapitulating cellular senescence that is the central pathophysiological mechanism of chronic kidney disease (CKD).
Yuki Nakao, Makiko Mori, Yuta Sekiguchi, Iori Morita, Ryota Shindoh, Shintaro Mandai, Tamami Fujiki, Hiroaki Kikuchi, Fumiaki Ando, Koichiro Susa, Takayasu Mori, Ayumi Suzuki, Yuji Nashimoto, Hirokazu Kaji, Yuma Waseda, Soichiro Yoshida, Yasuhisa Fujii, Eisei Sohara, Shinichi Uchida, Kensuke Miyake, Yutaro Mori   +20 more
wiley   +1 more source