Results 251 to 260 of about 1,617,447 (322)

Identification and Validation of qRT-PCR Reference Genes for Analyzing Arabidopsis Responses to High-Temperature Stress. [PDF]

Curr Issues Mol Biol
Chen S, Cai Q, Liu P, Liu J, Chen G, Yan H, Zheng H.   +6 more
europepmc   +1 more source

Tubule‐Derived IFN‐α Promotes GSDMD‐Mediated Macrophage Pyroptosis to Drive Renal Inflammation and Fibrosis Through JAK2/STAT2 Activation

Advanced Science, EarlyView.
Mao and colleagues uncover a STAT2/IRF9‐dependent signaling axis through which tubular epithelial cell (TEC)‐derived IFN‐α induces gasdermin D (GSDMD)‐mediated pyroptosis in macrophages. This TEC‐macrophage feedback loop amplifies renal inflammation and fibrosis, providing mechanistic insight into the progression of chronic kidney disease and revealing
Yiping Xu, Yating Wang, Siming Jiang, Yi Li, Guanglan Li, Yuchu Liu, Siyuan Li, Yiming Zhou, Qinghua Liu, Yi Zhou, Wei Chen, Hongyu Li, Haiping Mao   +12 more
wiley   +1 more source

Application of novel dark-quencher labeled probes in multiplex qRT-PCR assays for rapid detection of SARS-CoV-2 variants. [PDF]

J Thorac Dis
Zeng Z, Yang J, Dai J, Zou L, Lin Z, Liu Y, Guan W, Li F, Zheng K, Yuan S, Sun F, He F, Hong Y, Li H, Liu W, Men G, Zhang X, Lan Y, Deng X, Li L, Lin Y, Lai H, Qian P, Fan Q, Jiang M, Li J, Tang G, Mo Q, Deng X, Huang J, Deng X, Yang Z.   +31 more
europepmc   +1 more source

实时荧光定量PCR（qRT-PCR）

BIO-PROTOCOL, 2023
openaire   +1 more source

Structure‐Based Development of Ultra‐Broad‐Spectrum 3C‐Like Protease Inhibitors

Advanced Science, EarlyView.
This study provides an in‐depth analysis of the substrate binding pocket of 3CLpros across all coronavirus species using bioinformatics and structural insights, revealing the critical impact of S2/S4 subsite diversity on the broad‐spectrum activity of approved therapeutics.
Haixia Su, Tianqing Nie, Guofeng Chen, Muya Xiong, Yumin Zhang, Guoqing Wu, Mengyuan You, Hang Xie, Jian He, Yanchao Xiong, Hangchen Hu, Wenfeng Zhao, Minjun Li, Gengfu Xiao, Leike Zhang, Yechun Xu   +15 more
wiley   +1 more source

Screening and Validation of Stable Reference Genes for qRT-PCR Analysis in Epicauta gorhami (Coleoptera: Meloidae). [PDF]

Insects
Yang G, Yu X, Zhang Y, Luo J, Li X, Zhu L, Zhang H, Jin L, Wu G, Yan X, Shen C.   +10 more
europepmc   +1 more source

Microglial Fkbp5 Impairs Post‐Stroke Vascular Integrity and Regeneration by Promoting Yap1‐Mediated Glycolysis and Oxidative Phosphorylation

Advanced Science, EarlyView.
A post‐stroke perivascular niche of microglia characterized by low expression of M2 markers and elevated glycolysis, oxidative phosphorylation (OXPHOS), and phagocytic activity is identified, which is termed stroke‐activated vascular‐associated microglia (stroke‐VAM).
Yanan Li, Yanmei Qiu, Yunlei Yang, Yanhao Wei, Haokun Peng, Longhai Zeng, Pengcheng Li, Rentang Bi, Bo Hu   +8 more
wiley   +1 more source

Reference Genes for Expression Analyses by qRT-PCR in Enterobacter cancerogenus. [PDF]

Microorganisms
Pan Y, Zhao Y, Zeng HR, Wu JQ, Song YY, Rao YH, Li GQ, Jin L.   +7 more
europepmc   +1 more source

PTG‐Dependent Glycogen Metabolic Dysfunction Drives Impaired Adipose Browning: A Novel Mechanism Linking PM2.5 to Metabolic Disorders

Advanced Science, EarlyView.
This study provides the first evidence that PM2.5 impairs iWAT browning via PTG‐mediated glycogen metabolism disruption, which is initiated by ADRB3 inhibition and subsequently triggers VEGFB upregulation. It thereby delineates the ADRB3‐PTG‐VEGFB axis as central to PM2.5‐induced metabolic dysfunction and identifies adipose glycogen metabolism as a ...
Limin Wang, Renjie Hu, Yanxi Chai, Ping He, Sanduo Li, Lisha Zhao, Wenbin Zhao, Lu Zhang, Li Qin, Ran Li, Xiaoli Hou, Qinghua Sun, Cuiqing Liu   +12 more
wiley   +1 more source