Results 31 to 40 of about 1,079,378 (256)

Physical Simulation-Based Calibration for Quantitative Real-Time PCR

Applied Sciences
The fluorescence quantitative polymerase chain reaction (qPCR) instrument has been widely used in molecular biology applications, where the reliability of the qPCR performance directly affects the accuracy of its detection results.
Tianyu Zhu, Xin Liu, Xinqing Xiao
doaj   +1 more source

Quantitative real-time PCR data analysis with R [PDF]

, 2016
This dissertation covers an introduction to the field of qPCR data analysis using the state-of-the-art R programming language. qPCR analyses genomic data based on the DNA replication.
Machado de Souza, Ignacio Nicolás
core   +1 more source

Transferrin receptor 1‐mediated iron uptake supports thermogenic activation in human cervical‐derived adipocytes

FEBS Letters, EarlyView.
In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai, Mizuki Seo, Gyath Karadsheh, Fachrur Rizal Mahendra, Máté Á Demény, Ferenc Győry, János András Mótyán, László Fésüs, Endre Kristóf, Rini Arianti   +9 more
wiley   +1 more source

Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction [PDF]

, 2005
Aim: To examine the sensitivity and accuracy of real-time polymerase chain reaction (PCR) for the quantification of hepatitis B virus (HBV) DNA in semen.
Chen, Y, He, ML, Kung, HF, Li, Z, Lin, MC, Lu, GX, Peng, Y, Qian, WP, Shing, LK, Tan, YQ   +9 more
core   +1 more source

The neural crest‐associated gene ERRFI1 is involved in melanoma progression and resistance toward targeted therapy

Molecular Oncology, EarlyView.
ERRFI1, a neural crest (NC)‐associated gene, was upregulated in melanoma and negatively correlated with the expression of melanocytic differentiation markers and the susceptibility of melanoma cells toward BRAF inhibitors (BRAFi). Knocking down ERRFI1 significantly increased the sensitivity of melanoma cells to BRAFi.
Nina Wang, Qian Sun, Daniel Novak, Lei Zhu, Juliane Poelchen, Tamara Steinfass, Yiman Wang, Viktor Umansky, Jochen Utikal   +8 more
wiley   +1 more source

Investigations into the utility of real-time PCR for the detection, quantitation and characterisation of clinically relevant viruses [PDF]

, 2003
The use of PCR as a tool for the diagnostic virology and viral research laboratories has greatly increased in recent years, however the use of conventional PCR and amplicon detection systems can be a complex and relatively slow process that increases the
Mackay, Ian M.
core   +1 more source

Exploiting metabolic adaptations to overcome dabrafenib treatment resistance in melanoma cells

Molecular Oncology, EarlyView.
We show that dabrafenib‐resistant melanoma cells undergo mitochondrial remodeling, leading to elevated respiration and ROS production balanced by stronger antioxidant defenses. This altered redox state promotes survival despite mitochondrial damage but renders resistant cells highly vulnerable to ROS‐inducing compounds such as PEITC, highlighting redox
Silvia Eller, Susanne Ebner, Carmen Haselrieder, Julia K. Günther, Astrid Drasche, Sophie Strich, Chiara Volani, Andrea Medici, Aleksandar Nikolajevic, Alex Deltedesco, Johannes E. Sigmund, Michael J. Blumer, Martin Hermann, Johanna Vanacker, Gerald Brandacher, Eduard Stefan, Omar Torres‐Quesada, Jakob Troppmair   +17 more
wiley   +1 more source

Quantitative real-time PCR for the clinical detection of Helicobacter pylori

Genetics and Molecular Biology, 2007
Accurate diagnosis of Helicobacter pylori infection is very important in both clinical practice and research. We evaluated the sensitivity of real-time PCR (RT-PCR) for the detection and quantification of Helicobacter pylori using DNA from 91 human ...
Marcelo Lima Ribeiro, Christina Cunha Ecclissato, Ricardo Gabriel Mattos, Sergio Mendonca, José Pedrazzoli Jr.   +4 more
doaj   +1 more source

Evaluation and validation of reference genes in Cymbidium faberi for real-time quantitative PCR

BioTechniques, 2022
For all organs at all Cymbidium faberi stages, ACT, UBQ3 and GAPDH can be selected as reference genes. For organs of the vegetative stage, UBQ2 and UBQ3 can be chosen for analysis of normalized gene expression. For the bud stage, ACT and UBQ3 can be used
Yunfang Tian, Zhigang Chu, Linqing Wang, Huiyu Wang, Xiuyun Yuan, Si Wu, Yuzhen Yang   +6 more
doaj   +1 more source

A multiplex quantitative real time PCR to detect Xanthomonas axonopodis pv.allii from onion seeds : [P2-26] [PDF]

, 2010
Bacterial blight of onion is a seed-borne emerging disease threatening world onion production, and causing damage to other Allium crops. Its causal agent, #Xanthomonas axonopodis pv.
Escalon, Aline, Jouen, Emmanuel, Pruvost, Olivier, Robène-Soustrade, Isabelle   +3 more
core