Results 21 to 30 of about 1,079,378 (256)

Improving statistical inference on pathogen densities estimated by quantitative molecular methods: malaria gametocytaemia as a case study [PDF]

, 2015
BACKGROUND: Quantitative molecular methods (QMMs) such as quantitative real-time polymerase chain reaction (q-PCR), reverse-transcriptase PCR (qRT-PCR) and quantitative nucleic acid sequence-based amplification (QT-NASBA) are increasingly used to ...
A Gelman, André Lin Ouédraogo, B Hoadley, C Eisenhart, C Fraser, C Osborne, CE McCulloch, Cornelus Hermsen, D Lunn, DJ Lunn, DJ Spiegelhalter, DL Massart, EC Fieller, F Ferre, FA Graybill, G Marsaglia, GK Shulka, HG Niesters, HS Mubarak El, J Jiang, J Wain, JF Cohen, JH McCulloch, JM Ruijter, JN Miller, JO Beer De, JS Murray, JS Yuan, K Danzer, K Khairnar, KK Lai, LC Okell, LJ Naszódi, M Plummer, M Sivaganesan, M Woodhead, Martin Walker, María-Gloria Basáñez, NM Ferguson, P Schneider, P Schneider, P Vankeerberghen, PF Mens, R Wampfler, S Nadarajah, SA Bustin, SA Bustin, SA Fiscus, T Nolan, T Ponnudurai, TD Schmittgen, Teun Bousema, Thomas S Churcher, TS Churcher, World Health Organization, Y Karlen, YW Tang   +56 more
core   +4 more sources

FungiQuant: A broad-coverage fungal quantitative real-time PCR assay

BMC Microbiology, 2012
Background Fungal load quantification is a critical component of fungal community analyses. Limitation of current approaches for quantifying the fungal component in the human microbiome suggests the need for new broad-coverage techniques.
Liu Cindy M, Kachur Sergey, Dwan Michael G, Abraham Alison G, Aziz Maliha, Hsueh Po-Ren, Huang Yu-Tsung, Busch Joseph D, Lamit Louis J, Gehring Catherine A, Keim Paul, Price Lance B   +11 more
doaj   +1 more source

DETERMINAZIONI VIRALI QUANTITATIVE MEDIANTE “PCR REAL TIME” CON IL SISTEMA “ROTOR GENETM”

Microbiologia Medica, 2005
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M.G. Marin, P. Turconi, F. Poma, F. Calamani, P. Guarneri, L. Berardi, L. Lattuada   +6 more
doaj   +1 more source

Development of a Real-Time Quantitative PCR Based on a TaqMan-MGB Probe for the Rapid Detection of Theileria haneyi

Microorganisms, 2023
Equine piroplasmosis (EP) is a parasitic disease caused by Theileria equi (T. equi), Babesia caballi (B. caballi) and Theileria haneyi (T. haneyi). This disease is considered to be reportable by the World Organization for Animal Health (WOAH).
Bingqian Zhou, Guangpu Yang, Zhe Hu, Kewei Chen, Wei Guo, Xiaojun Wang, Cheng Du   +6 more
doaj   +1 more source

The performance of semi-quantitative differential PCR is similar to that of real-time PCR for the detection of the MYCN gene in neuroblastomas

Brazilian Journal of Medical and Biological Research, 2009
Amplification of the MYCN gene in neuroblastomas is a potent biological marker of highly aggressive tumors, which are invariably fatal unless sound clinical management is applied. To determine the usefulness of semi-quantitative differential PCR (SQ-PCR)
A.C.M.F. Souza, D.R.V. Souza, S.S. Sanabani, R.R. Giorgi, I. Bendit   +4 more
doaj   +1 more source

Detection and quantification of Verticillium dahliae and V. longisporum by droplet digital PCR versus quantitative real-time PCR

Frontiers in Cellular and Infection Microbiology, 2022
Vascular wilt, caused by Verticillium dahliae and V. longisporum, limits the quality and yield of agricultural crops. Although quantitative real-time PCR (qPCR) has greatly improved the diagnosis of these two pathogens over traditional, time-consuming ...
Di Wang, Xinya Jiao, Haijiang Jia, Shumei Cheng, Xi Jin, Youhua Wang, Yunhua Gao, Xiaofeng Su   +7 more
doaj   +1 more source

The prognostic impact of EGFR, ErbB2 and MET gene amplification in human gastric carcinomas as measured by quantitative Real-Time PCR [PDF]

, 2015
Purpose: Identification of critical genes which play pivotal roles in controlling tumor growth and survival will establish the basis for developing therapeutic targets. In this study, we focused on frequencies of EGFR, ErbB2 and MET gene amplification in
Farazmandfar, T., Janbabai, G., Naghshvar, F., Oladi, Z., Taghvaei, T.   +4 more
core   +1 more source

Evaluation and comparison of SYBR Green I Real-Time PCR and TaqMan Real-Time PCR methods for quantitative assay of Listeria monocytogenes in nutrient broth and milk [PDF]

, 2012
Specific traditional plate count method and real-time PCR systems based on SYBR Green I and TaqMan technologies using a specific primer pair and probe for amplification of iap-gene were used for quantitative assay of Listeria monocytogenes in seven ...
Bassami, M R, Dadkhah, H, Hashemi, S, Hosseini, H, Karatzas, Kimon Andreas G., Khaksar, R, Shahraz, F   +6 more
core   +1 more source

Differential cytokine responses following Marek's disease virus infection in chicken genotypes differing in resistance to Marek's disease [PDF]

, 2003
The production of cytokine mRNAs, in addition to viral DNA, was quantified by real-time quantitative reverse transcription-PCR (RT-PCR) (cytokines) or PCR (virus) in splenocytes during the course of Marek's disease virus (MDV) infection in four inbred ...
Davison, T F, Kaiser, Pete, Underwood, G
core   +1 more source

Development of a droplet digital PCR method for detection of porcine circovirus 4

BMC Veterinary Research, 2023
Background Porcine circovirus 4 (PCV4), a newly emerging virus that was first discovered in 2019, may pose a potential threat to the pig industry. Droplet digital PCR (ddPCR) is an absolute quantitative method that has high sensitivity and accuracy.
Yangkun Liu, Xinru Zhang, Xueying Han, Jiaxing Liu, Lunguang Yao   +4 more
doaj   +1 more source