Results 11 to 20 of about 1,079,378 (256)

Differential gene expression in response to cold stress in Lepidium apetalum during seedling emergence

Biologia Plantarum, 2012
Germination of Lepidium apetalum Wild. seeds is invariably arrested by cold stress. cDNA-amplified fragment length polymorphism (AFLP) technique was used to isolate genes relevant to chilling stress (4 °C) during seedling emergence. 43 transcript-derived
H. X. Zhao, Q. Li, G. Li, Y. Du
doaj   +1 more source

Detection of Salmonella Typhi in Bile by Quantitative Real-Time PCR

Microbiology Spectrum, 2022
In countries where the incidence of typhoid fever is high, fecal material from short-term carriers of Salmonella Typhi contaminates inadequately treated water supplies.
Ellen E. Higginson, Joseph Nkeze, Jasnehta Permala-Booth, Irene N. Kasumba, Rosanna Lagos, Juan Carlos Hormazabal, Alexander Byrne, Gad Frankel, Myron M. Levine, Sharon M. Tennant   +9 more
doaj   +1 more source

Low-Cost Battery-Powered and User-Friendly Real-Time Quantitative PCR System for the Detection of Multigene

Micromachines, 2020
Real-time polymerase chain reaction (PCR) is the standard for nucleic acid detection and plays an important role in many fields. A new chip design is proposed in this study to avoid the use of expensive instruments for hydrophobic treatment of the ...
Junru An, Yangyang Jiang, Bing Shi, Di Wu, Wenming Wu   +4 more
doaj   +1 more source

Detection of Pork-Derived Ingredients Based on Real-time Quantitative PCR

Shipin gongye ke-ji, 2022
In order to accurately and reliably quantify pork-derived ingredients in meat products, single-copy gene (carcinoembryonic antigen-related cell adhesion molecule 2-like, CACA) in pig cell nucleus was screened by bioinformatics methods, with CACA gene as ...
Yao YANG, Nengwu SI, Yuao YAN, Rui QIN, Zhihua WU, Li ZHANG   +5 more
doaj   +1 more source

RTPrimerDB : the portal for real-time PCR primers and probes [PDF]

, 2009
RTPrimerDB (http://www.rtprimerdb.org) is a freely accessible database and analysis tool for real-time quantitative PCR assays. RTPrimerDB includes records with user submitted assays that are linked to genome information from reference databases and ...
S. Lefever, J. Vandesompele, F. Speleman, F. Pattyn, Pattyn, Pattyn, Aranyi, Hellemans   +7 more
core   +2 more sources

Preliminary screening of intestinal barrier genes associated with porcine epidemic diarrhea virus infection in pigs

Revista Brasileira de Zootecnia, 2020
To screen intestinal barrier genes associated with porcine epidemic diarrhea virus (PEDV) infection, in the present study we first detected PEDV-infected piglets ( Sus scrofa ) with intestinal damage using quantitative real-time PCR (qPCR) and ...
Xiaoyi Qi, Yafei Xu, Weiyun Qin, Haifei Wang, Shenglong Wu, Wenbin Bao   +5 more
doaj   +1 more source

A quantitative real-time PCR method for absolute telomere length

BioTechniques, 2008
Telomere shortening is an important risk factor for cancer and accelerated aging. Here we describe the development of a simple and reproducible method to measure absolute telomere length. Based on Cawthon's quantitative real-time PCR (qRT-PCR) assay, our
Nathan J. O'Callaghan, Varinderpal S. Dhillon, Philip Thomas, Michael Fenech   +3 more
doaj   +1 more source

Presence of SARS-CoV-2 in human tears detected by quantitative real time PCR (qRT-PCR)

Medical Journal of Dr. D.Y. Patil Vidyapeeth, 2022
Background: COVID-19 has caused a pandemic since the end of the year 2019. Controversy regarding the presence of the SARS-CoV-2 in tears and conjunctival sac has created suspense throughout.
Neeta Mishra, Chandrashekhar Raut, Neelam Prasad, J S Bhawalkar, Safia Farooqui, Shahzad Mirza, Veshal V Madan   +6 more
doaj   +1 more source

Differential expression of cellulose synthase (CesA) gene transcripts in potato as revealed by QRT-PCR [PDF]

, 2009
Two transgenic potato lines, csr2–1 and csr4–8 that contained two different antisense cellulose synthase (CesA) genes, csr2 and csr4, respectively were crossed.
Jacobsen, E., Olawole, O., Vincken, J.P., Visser, R.G.F.   +3 more
core   +2 more sources

Exogenous Reference RNA for Normalization of Real-Time Quantitative PCR

BioTechniques, 2003
We have utilized an in vitro transcribed 3′ mRNA fragment of the plant gene ribulose bisphosphate carboxylase (RuBisCO) as an exogenous standard for normalization of quantitative PCR data. Both K562 cells and primary erythroid CD34+ progenitor cells were
Reginald D. Smith, Benjamin Brown, Pranvera Ikonomi, Alan N. Schechter   +3 more
doaj   +1 more source