Quantitative analysis of the dystrophin gene by real-time PCR [PDF]
Archives of Biological Sciences, 2012 Duchenne and Becker muscular dystrophy (DMD/BMD) are severe X-linked neuromuscular disorders caused by mutations in the dystrophin gene. Our aim was to optimize a quantitative real-time PCR method based on SYBR® Green I chemistry for routine ...
Maksimovic Nela +12 more
doaj +4 more sources
Quantitative real time PCR for distinction between Pneumocystis jirovecii infection/colonization in hospitalized patients [PDF]
Frontiers in Cellular and Infection MicrobiologyBackgroundIdentification of the opportunistic fungus Pneumocystis jirovecii in respiratory specimens presents challenges, particularly in differentiating between colonization and active infection.
Faezeh Rouhi +9 more
doaj +2 more sources
An inter-laboratory validation of a real time PCR assay to measure host excretion of bacterial pathogens, particularly of mycobacterium bovis [PDF]
, 2011 Advances in the diagnosis of Mycobacterium bovis infection in wildlife hosts may benefit the development of sustainable approaches to the management of bovine tuberculosis in cattle.
Travis, Emma Rachel +16 more
core +6 more sources
Molecular Diagnostics in the Mycosphaerella Leaf Spot Disease Complex of Banana and for Radopholus similis [PDF]
, 2009 Mycosphaerella leaf spots and nematodes threaten banana cultivation worldwide. The Mycosphaerella disease complex involves three related ascomycetous fungi: Mycosphaerella fijiensis, M. musicola and M. eumusae.
Araya Vargas, M. +8 more
core +6 more sources
Fermentation, 2022 Rummeliibacillus sp. TYF-LIM-RU47, isolated from the fermentation substrate of grain vinegar, could produce acetoin using a variety of carbon sources, including pentose, hexose and lignocellulose.
Guoyang Feng +5 more
doaj +1 more source
Detection of 1p19q Deletion by Real-Time Comparative Quantitative PCR
Biomarker Insights, 2012 1p/19q (1p and/or 19q) deletions are prognostic factors in oligodendroglial tumors (OT) and predict better survival after both chemotherapy and radiotherapy. While studying 1p/19q status as a potential variable within multivariate prognosis models for OT,
Abhishek Chaturbedi +3 more
doaj +2 more sources
Molecular Plant-Microbe Interactions, 2003 Significant changes in root morphology and physiology during arbuscular mycorrhiza (AM) development are likely to be controlled by specific gene expression pattern in the host plant.
Anne Wulf +9 more
doaj +1 more source
Real-time reverse transcription polymerase chain reaction development for rapid detection of Tomato brown rugose fruit virus and comparison with other techniques [PDF]
, 2019 Background: Tomato brown rugose fruit virus (ToBRFV) is a highly infectious tobamovirus that causes severe disease in tomato (Solanum lycopersicum L.) crops.
Alfaro-Fernandez A. +5 more
core +1 more source
Design and evaluation of 16S rRNA sequence based oligonucleotide probes for the detection and quantification of Comamonas testosteroni in mixed microbial communities [PDF]
, 2006 Background The β-proteobacterial species Comamonas testosteroni is capable of biotransformation and also biodegradation of a range of chemical compounds and thus potentially useful in chemical manufacturing and bioremediation.
Bathe, Stephan, Hausner, Martina
core +1 more source
Real-Time PCR Assay for Quantitative Mismatch Detection
BioTechniques, 2003 We describe here a quantitative realtime PCR assay for the detection of single-base-pair differences that does not require fluorescently labeled gene-specific probes or complicated primer combinations.
L. Shively +5 more
doaj +1 more source