PLoS ONE, 2018 The oriental armyworm, Mythimna separata, is a major insect pest in China and other Asian countries. Unfortunately, suitable reference genes for quantitative real-time PCR (qRT-PCR) have not been previously identified in M.
Hongbo Li +5 more
semanticscholar +1 more source
Differential cytokine responses following Marek's disease virus infection in chicken genotypes differing in resistance to Marek's disease [PDF]
, 2003 The production of cytokine mRNAs, in addition to viral DNA, was quantified by real-time quantitative reverse transcription-PCR (RT-PCR) (cytokines) or PCR (virus) in splenocytes during the course of Marek's disease virus (MDV) infection in four inbred ...
Davison, T F, Kaiser, Pete, Underwood, G
core +1 more source
Development of a droplet digital PCR method for detection of porcine circovirus 4
BMC Veterinary Research, 2023 Background Porcine circovirus 4 (PCV4), a newly emerging virus that was first discovered in 2019, may pose a potential threat to the pig industry. Droplet digital PCR (ddPCR) is an absolute quantitative method that has high sensitivity and accuracy.
Yangkun Liu +4 more
doaj +1 more source
Validation of internal control for gene expression study in soybean by quantitative real-time PCR
BMC Molecular Biology, 2008 BackgroundNormalizing to housekeeping gene (HKG) can make results from quantitative real-time PCR (qRT-PCR) more reliable. Recent studies have shown that no single HKG is universal for all experiments.
Bo Jian +5 more
semanticscholar +1 more source
Differential quantification of CYP2D6 gene copy number by four different quantitative real-time PCR assays [PDF]
, 2010 Copy number variations (CNVs) in the CYP2D6 gene contribute to interindividual variation in drug metabolism. As the most common duplicated allele in Asian populations is the nonfunctional CYP2D6*36 allele, the goal of this study was to identify CNV ...
Flockhart, David A. +5 more
core +1 more source
Physical Simulation-Based Calibration for Quantitative Real-Time PCR
Applied SciencesThe fluorescence quantitative polymerase chain reaction (qPCR) instrument has been widely used in molecular biology applications, where the reliability of the qPCR performance directly affects the accuracy of its detection results.
Tianyu Zhu, Xin Liu, Xinqing Xiao
doaj +1 more source
Evaluation and validation of reference genes in Cymbidium faberi for real-time quantitative PCR
BioTechniques, 2022 For all organs at all Cymbidium faberi stages, ACT, UBQ3 and GAPDH can be selected as reference genes. For organs of the vegetative stage, UBQ2 and UBQ3 can be chosen for analysis of normalized gene expression. For the bud stage, ACT and UBQ3 can be used
Yunfang Tian +6 more
doaj +1 more source
Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
FEBS Letters, EarlyView.Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source
Quantitative real-time PCR data analysis with R [PDF]
, 2016 This dissertation covers an introduction to the field of qPCR data analysis using the state-of-the-art R programming language. qPCR analyses genomic data based on the DNA replication.
Machado de Souza, Ignacio Nicolás
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First detection of Waddlia chondrophila in Africa using SYBR Green real-time PCR on veterinary samples. [PDF]
, 2013 Waddlia chondrophila is a strict intracellular microorganism belonging to the order Chlamydiales that has been isolated twice from aborted bovine fetuses, once in USA and once in Germany. This bacterium is now considered as an abortigenic agent in cattle.
Barkallah, M. +5 more
core +1 more source