A rapid culture independent methodology to quantitatively detect and identify common human bacterial pathogens associated with contaminated high purity water [PDF]
, 2015 Background: Water and High Purity Water (HPW) distribution systems can be contaminated with human pathogenic microorganisms. This biocontamination may pose a risk to human health as HPW is commonly used in the industrial, pharmaceutical and clinical ...
Barry, Thomas +4 more
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Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process [PDF]
, 2008 12 pages, 2 figures, 4 tables.-- PMID: 19102748 [PubMed].-- PMCID: PMC2629474.[Background] The elucidation of gene expression patterns leads to a better understanding of biological processes.
Marino Expósito-Rodríguez +3 more
core +2 more sources
Barrier protection via Toll-like receptor 2 signaling in porcine intestinal epithelial cells damaged by deoxynivalnol [PDF]
, 2016 Additional file 2. IPEC-J2 cells pretreated with TLR2 ligand maintained the expression of MCP-1, GM-CSF and TLR2 against DON exposure. IPEC-J2 cells pretreated with or without TLR2 ligand for 24 h were exposed to DON.
Byung-Chul Park +11 more
core +8 more sources
Polymerase chain displacement reaction
BioTechniques, 2013 Quantitative PCR assays are now the standard method for viral diagnostics. These assays must be specific, as well as sensitive, to detect the potentially low starting copy number of viral genomic material.
Claire L. Harris +4 more
doaj +1 more source
Ex vivo screening for immunodominant viral epitopes by quantitative real time polymerase chain reaction (qRT-PCR) [PDF]
, 2003 The identification and characterization of viral epitopes across the Human Leukocyte Antigen (HLA) polymorphism is critical for the development of actives-specific or adoptive immunotherapy of virally-mediated diseases.
Nagorsen Dirk +5 more
core +2 more sources
BioTechniques, 1998 To detect t(14;18)-positive cells present in human lymphoma tissue, bone marrow aspirates and peripheral blood mononuclear cells (PBMNC), we have established an automated, real-time quantitative PCR using double-labeled fluorogenic probes. In relation to
Dölken Lars +2 more
doaj +1 more source
Soil Baiting, Rapid PCR Assay and Quantitative Real TIME PCR to Diagnose Late Blight of Potato in Quarantine Programs [PDF]
, 2018 Phytophthora infestans (mont) de Bary is a pathogen of great concern across the globe, and accurate detection is an important component in responding to the outbreaks of potential disease.
Anwar, F. (Firoz) +2 more
core +2 more sources
Virology Journal, 2018 Background Human adenovirus (HAdV) serotypes 2, 3 and 7 are more prevalent than other serotypes and have been associated with severe pneumonia in pediatric children.
Fang-zhou Qiu +10 more
doaj +1 more source
Enhanced cardiac expression of two isoforms of matrix metalloproteinase-2 in experimental diabetes mellitus. [PDF]
, 2019 BackgroundDiabetic cardiomyopathy (DM CMP) is defined as cardiomyocyte damage and ventricular dysfunction directly associated with diabetes independent of concomitant coronary artery disease or hypertension.
Ahn, Jinhee +15 more
core +2 more sources
Standard and Light-Cycler PCR methods for animal DNA species detection in animal feedstuffs [PDF]
, 2008 In this work four species-specific primers and probes were designed and evaluated for the detection and quantification of bovine, ovine, swine and chicken mitochondrial DNA in feeds.
Ajmone_Marsan, P +12 more
core +1 more source