Results 81 to 90 of about 1,061,192 (206)

Identification of circulating miRNA biomarkers based on global quantitative real-time PCR profiling

open access: yesJournal of Animal Science and Biotechnology, 2012
MicroRNAs (miRNAs) are small noncoding RNAs (18-25 nucleotides) that regulate gene expression at the post-transcriptional level. Recent studies have demonstrated the presence of miRNAs in the blood circulation.
Kang Kang   +3 more
doaj   +1 more source

[Real time quantitative PCR].

open access: yesExperimental & molecular medicine, 2002
So far, quantitative techniques, such as PCR and FISH, have been used to detect of DNA and RNA. However, it is difficult to measure and compare the exact amount of amplified products with the results of endpoint analysis in conventional PCR techniques.
openaire   +1 more source

自发性高血压大鼠血液miRNAs表达分析

open access: yesZhongguo shiyan zhenduanxue, 2014
目的本试验检测自发性高血压大鼠(SHR)血液中miR-448-5p、miR-128、miR-146a和miR-24-2*四种小分子RNA(miRNA)的表达,探讨高血压疾病与miRNA表达水平相关性,为人类高血压诊断及治疗提供新的靶标。方法应用荧光定量PCR方法对SHR大鼠和正常大鼠血液中miR-448-5p、miR-128、miR-146a和miR-24-2*表达量进行检测。结果与正常对照组相比,SHR大鼠血液中miR-448-5p、miR-128、miR-146a和miR-24-2 ...
鞠传静   +4 more
doaj  

Comparison of digital PCR and real time PCR methods for quantitative analysis of African Swine Fever Virus

open access: yesFrontiers in Veterinary Science
IntroductionThe African swine fever (ASF) is a viral disease of significant socio-economic and animal health impact, commonly diagnosed using molecular techniques.
Silvia Dei Giudici   +7 more
doaj   +1 more source

Real-time quantitative PCR (qPCR) v1

open access: yes, 2018

Chunfu Zheng   +3 more
openaire   +1 more source

Transcription of nanos-1 in Zebrafish Embryos is not Affected by Bisphenol A: Evaluated Using Quantitative Real-Time PCR [PDF]

open access: yes, 2019
The presence of primordial germ cells (PGCs) is crucial for proper gonad formation in zebrafish (Danio rerio). The many aspects of PGC migration that allow these cells to reach the proper location at the gonadal ridge include receptors, ligands, germ ...
Babich, Bridget   +4 more
core   +1 more source

Determinazione quantitativa di HCV-RNA: valutazione comparativa dei saggi Abbott Real-Time e Versant bDNA v.3

open access: yesMicrobiologia Medica, 2007
Hepatitis C virus (HCV) RNA measurement before, during and after antiviral therapy has become an essential tool in the management of interferon-based treatment of HCV-related infections.
Aldo Manzin   +4 more
doaj   +1 more source

Correlating Gene Transfection Efficiency and the Physical Properties of Various Cationic Poly(methacrylate) Systems [PDF]

open access: yes, 2006
Transfection efficiencies of several polymeric gene carriers were compared and correlated quantitatively to the amounts of cellular accumulation of plasmid DNA and to the expression of mRNA by quantitative real time PCR.
Hatton, T. Alan   +3 more
core  

Identification and Characterization of a Differentially Expressed Gene (07E12) in the Infective Larvae of the Parasitic Nematode Ascaris suum

open access: yesIranian Journal of Parasitology, 2014
Background: Parasitic nematodes cause animal and human diseases of major socio-economic importance worldwide. The suppression of parasite development at particular developmental stages could provide an alternative approach for nema-tode control.
Cuiqin Huang, Jingzhou You, Fangfang Nai
doaj  

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