Results 81 to 90 of about 1,315,258 (335)

Standard and Light-Cycler PCR methods for animal DNA species detection in animal feedstuffs [PDF]

, 2008
In this work four species-specific primers and probes were designed and evaluated for the detection and quantification of bovine, ovine, swine and chicken mitochondrial DNA in feeds.
Ajmone_Marsan, P, Chegdani, F, Chiappini, B, Faggionato, E, Fontana, C, Frezza, D, Giambra, V, Lanni, L, Losio, N, Maccabiani, G, Saccares, S, Vaccari, G, von Holst, C   +12 more
core   +1 more source

Implementation and Validation of the Roche Light Cycler 480 96-Well Plate Platform as a Real-Time PCR Assay for the Quantitative Detection of Cytomegalovirus (CMV) in Clinical Specimens Using the Luminex MultiCode ASRs System. [PDF]

, 2020
Allogenic stem-cell therapies benefit patients in the treatment of multiple diseases; however, the side effects of stem-cell therapies (SCT) derived from the concomitant use of immune suppression agents often include triggering infection diseases.
Li, Shengwen Calvin, Sender, Leonard S, Sparks, Kara J   +2 more
core   +1 more source

Identification and validation of stable reference genes for quantitative real time PCR in different minipig tissues at developmental stages

BMC Genomics, 2022
Background Quantitative real time PCR (qPCR) is a powerful tool to evaluate mRNA expression level. However, reliable qPCR results require normalization with validated reference gene(s).
Jeongah Song, Jeonghee Cho, Jeongsik Park, Jeong Ho Hwang   +3 more
doaj   +1 more source

Adaptaquin is selectively toxic to glioma stem cells through disruption of iron and cholesterol metabolism

Molecular Oncology, EarlyView.
Adaptaquin selectively kills glioma stem cells while sparing differentiated brain cells. Transcriptomic and proteomic analyses show Adaptaquin disrupts iron and cholesterol homeostasis, with iron chelation amplifying cytotoxicity via cholesterol depletion, mitochondrial dysfunction, and elevated reactive oxygen species.
Adrien M. Vaquié, Davod R. Shah, Eliane E. S. Brechbühl, Michael McNicholas, Zhaoyang Xu, John H. Stockley, Laura Morcom, Diana Gold Diaz, Gemma C. Girdler, Rachel V. Seear, Gabriel Balmus, Rajiv R. Ratan, Harry Bulstrode, James A. Nathan, Manav Pathania, Kevin M. Brindle, David H. Rowitch   +16 more
wiley   +1 more source

Soil Baiting, Rapid PCR Assay and Quantitative Real TIME PCR to Diagnose Late Blight of Potato in Quarantine Programs [PDF]

, 2018
Phytophthora infestans (mont) de Bary is a pathogen of great concern across the globe, and accurate detection is an important component in responding to the outbreaks of potential disease.
Anwar, F. (Firoz), Hussain, T. (Touseef), Singh, B. P. (Bir)   +2 more
core   +2 more sources

Selection of Suitable Reference Genes for Quantitative Real-Time PCR Normalization in Three Types of Rat Adipose Tissue

International Journal of Molecular Sciences, 2016
Quantitative real-time PCR (qRT-PCR) is the most classical technique in the field of gene expression study. This method requires an appropriate reference gene to normalize mRNA levels.
Wan-xia Zhang, Jie Fan, Jing-e Ma, Yi-Song Rao, Li Zhang, You-e Yan   +5 more
semanticscholar   +1 more source

Aggressive prostate cancer is associated with pericyte dysfunction

Molecular Oncology, EarlyView.
Tumor‐produced TGF‐β drives pericyte dysfunction in prostate cancer. This dysfunction is characterized by downregulation of some canonical pericyte markers (i.e., DES, CSPG4, and ACTA2) while maintaining the expression of others (i.e., PDGFRB, NOTCH3, and RGS5).
Anabel Martinez‐Romero, Ane Martinez‐Larrinaga, Joaquim Grego‐Bessa, Saioa Garcia‐Longarte, Hielke van Splunder, Ianire Astobiza, Amaia Ercilla, Laura Bozal‐Basterra, Isabel Mendizabal, Pilar Villacampa, Arkaitz Carracedo, Mariona Graupera   +11 more
wiley   +1 more source

Selection and validation of reference genes for the normalization of quantitative real-time PCR in different muscle tissues of rabbits

BMC Zoology, 2022
Background In molecular biology studies, the selection of optimal reference genes is of vital importance for accurately quantifying gene expression.
Mengke Ni, Zhichao Li, Jing Li, Hui He, Yaling Wang, Yixuan Jiang, Xianwei Wang, Zhuanjian Li, Ming Li, Huifen Xu   +9 more
doaj   +1 more source

Quantitative real-time PCR protocol for analysis of nuclear receptor signaling pathways

Nuclear Receptor Signaling, 2003
A major goal of the Nuclear Receptor Signaling Atlas (NURSA) is to elucidate the biochemical and physiological roles of nuclear receptors in vivo. Characterizing the tissue expression pattern of individual receptors and their target genes in whole ...
A. Bookout, D. Mangelsdorf
semanticscholar   +1 more source

The neural crest‐associated gene ERRFI1 is involved in melanoma progression and resistance toward targeted therapy

Molecular Oncology, EarlyView.
ERRFI1, a neural crest (NC)‐associated gene, was upregulated in melanoma and negatively correlated with the expression of melanocytic differentiation markers and the susceptibility of melanoma cells toward BRAF inhibitors (BRAFi). Knocking down ERRFI1 significantly increased the sensitivity of melanoma cells to BRAFi.
Nina Wang, Qian Sun, Daniel Novak, Lei Zhu, Juliane Poelchen, Tamara Steinfass, Yiman Wang, Viktor Umansky, Jochen Utikal   +8 more
wiley   +1 more source